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AB212086

Human CD44 ELISA Set

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Human CD44 ELISA Set is a Sandwich ELISA for the measurement of Human CD44 ELISA Set in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

CD44, LHR, MDU2, MDU3, MIC4, CD44 antigen, CDw44, Epican, Extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, Phagocytic glycoprotein 1, Phagocytic glycoprotein I, ECMR-III, PGP-1, PGP-I

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Sandwich ELISA - Human CD44 ELISA Set (AB212086)
  • sELISA

Supplier Data

Sandwich ELISA - Human CD44 ELISA Set (AB212086)

Dose Response Curve (Logarithmic)

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.13 ng/mL

Range

0.31 - 10 ng/mL

Assay Platform

Reagents

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Secondary Antibodies

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Product details

Abcam's CD44 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) Set is designed for the quantitative measurement of soluble CD44 in Human serum, plasma, buffered solutions and cell culture medium.

The ELISA set contains 100 μg of unlabeled, mouse monoclonal capture antibody and 50 μg of mouse monoclonal detector antibody. Lyophilized Human CD44 protein purified from Human serum is included for quantification. The exact concentration of the protein standard can be found on the vials' label. Detection requires an secondary antibody labeled with biotin.

The protocol booklet contains guidelines on how to use the ELISA Set for a sandwich ELISA with a secondary antibody for detection.

This kit will recognize both endogenous and recombinant Human CD44.

Optimization of the ELISA Set reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this ELISA Set in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.

Optimization of the ELISA Set reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this ELISA Set in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.

Additional protocol information and tip on the use of the Human CD44 ELISA Set for sandwich ELISA can be found on our website.

For additional information on the performance of the antibody pair used in this ELISA Set, please see our equivalent CD44 Human ELISA Set (ab45912) which uses the same antibody pair.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab212086-100Test", "assetComponentDetails": [ { "size":"1 x 50 µg", "name":"Biotinylated anti-CD44 anitibody", "number":"AB212086-CMP03", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human CD44 Capture Antibody", "number":"AB212086-CMP01", "productcode":"" }, { "size":"1 x 139 ng", "name":"Human CD44 Standard Protein", "number":"AB212086-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Cell-surface receptor that plays a role in cell-cell interactions, cell adhesion and migration, helping them to sense and respond to changes in the tissue microenvironment (PubMed : 16541107, PubMed : 19703720, PubMed : 22726066). Participates thereby in a wide variety of cellular functions including the activation, recirculation and homing of T-lymphocytes, hematopoiesis, inflammation and response to bacterial infection (PubMed : 7528188). Engages, through its ectodomain, extracellular matrix components such as hyaluronan/HA, collagen, growth factors, cytokines or proteases and serves as a platform for signal transduction by assembling, via its cytoplasmic domain, protein complexes containing receptor kinases and membrane proteases (PubMed : 18757307, PubMed : 23589287). Such effectors include PKN2, the RhoGTPases RAC1 and RHOA, Rho-kinases and phospholipase C that coordinate signaling pathways promoting calcium mobilization and actin-mediated cytoskeleton reorganization essential for cell migration and adhesion (PubMed : 15123640). Upon interaction with LGALS9 ligand, activates downstream signaling components including LCK, ERK and MAPK to promotes NK cell activation (PubMed : 37006235).. (Microbial infection) Promotes foot-and-mouth disease virus internalization via macropinocytosis.
See full target information CD44
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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