Human CD46 ELISA Kit
- Recombinant
- SimpleStep
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Human CD46 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CD46 with a sensitivity of 9.4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
View Alternative Names
CD46, MCP, MIC10, Membrane cofactor protein, TLX, Trophoblast leukocyte common antigen
- sELISA
Supplier Data
Sandwich ELISA - Human CD46 ELISA Kit (AB283877)
Interpolated concentrations of native CD46 in human serum and plasma samples.
The concentrations of CD46 were measured in duplicates, interpolated from the CD46 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 5%, plasma (citrate) 5%, plasma (heparin) 5%, and plasma (EDTA) 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD46 concentration was determined to be 12.91 ng/mL in serum, 13.31 ng/mL in plasma (citrate), 15.04 ng/ml in plasma (heparin), and 11.27 ng/mL in plasma (EDTA).
- sELISA
Supplier Data
Sandwich ELISA - Human CD46 ELISA Kit (AB283877)
Interpolated concentrations of native CD46 in human HepG2 cell culture supernatant samples.
The concentrations of CD46 were measured in duplicates, interpolated from the CD46 standard curves and corrected for sample dilution. Undiluted samples are as follows : HepG2 supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD46 concentration was determined to be 2.93 ng/mL in HepG2 cell culture supernatant.
- sELISA
Supplier Data
Sandwich ELISA - Human CD46 ELISA Kit (AB283877)
Serum from ten individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD46 concentration was determined to be 14.05 ng/mL with a range of 8.25 – 20.61 ng/mL.
- sELISA
Supplier Data
Sandwich ELISA - Human CD46 ELISA Kit (AB283877)
Example of human CD46 standard curve in Sample Diluent NS.
The CD46 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Reactivity data
Product details
Human CD46 ELISA kit (ab283877) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human CD46 protein in human serum, plasma, and cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CD46 with 9.40 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
CD46 is a complement receptor and is involved in regulation of the complement system. CD46 inactivates complement components C3b and C4b. It can also act as a receptor for the Edmonston strain of the measles virus and several other viruses and bacteria. High levels of CD46 protein have been seen in medulloblastoma specimens, and vaccinations are in clinical trials for the treatment of medulloblastoma using the Edmonston strain of measles virus.
Precision
Recovery
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Beyond its complement regulatory role CD46 acts in immune response modulation and reproduction. It forms a part of a protein complex that involves members such as decay-accelerating factor and complement receptor 1. CD46 also engages in intracellular signaling that affects T cell function and differentiation. In the reproductive context it participates in sperm-oocyte fusion and has a role in trophoblast fusion during placental formation.
Pathways
CD46 integrates into various signaling pathways including the complement and T cell receptor signaling pathways. In the complement pathway CD46 interacts with C3b and factor I promoting cleavage and inactivation of C3b leading to pathway regulation. Involvement in the T cell receptor pathway hints that CD46 signaling protects against overactive immune responses by promoting a switch to a regulatory T cell phenotype which can modulate immunity and prevent autoimmunity.
Product protocols
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Target data
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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