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Human CD47 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human CD47 in Human in Serum, Plasma, Cell culture supernatant samples.

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Images

Sandwich ELISA - Human CD47 ELISA Kit (AB314832), expandable thumbnail

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

0.52 - 125 ng/mL

Sensitivity

= 0.52 ng/mL

Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Adhesive protein that mediates cell-to-cell interactions (PubMed:11509594, PubMed:15383453). Acts as a receptor for thrombospondin THBS1 and as modulator of integrin signaling through the activation of heterotrimeric G proteins (PubMed:19004835, PubMed:7691831, PubMed:8550562). Involved in signal transduction, cardiovascular homeostasis, inflammation, apoptosis, angiogenesis, cellular self-renewal, and immunoregulation (PubMed:11509594, PubMed:15383453, PubMed:19004835, PubMed:27742621, PubMed:32679764, PubMed:7691831, PubMed:8550562). Plays a role in modulating pulmonary endothelin EDN1 signaling (PubMed:27742621). Modulates nitrous oxide (NO) signaling, in response to THBS1, hence playing a role as a pressor agent, supporting blood pressure (By similarity). Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells (PubMed:11509594). Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation (PubMed:15383453). Positively modulates FAS-dependent apoptosis in T-cells, perhaps by enhancing FAS clustering (By similarity). Plays a role in suppressing angiogenesis and may be involved in metabolic dysregulation during normal aging (PubMed:32679764). In response to THBS1, negatively modulates wound healing (By similarity). Inhibits stem cell self-renewal, in response to THBS1, probably by regulation of the stem cell transcription factors POU5F1/OCT4, SOX2, MYC/c-Myc and KLF4 (By similarity). May play a role in membrane transport and/or integrin dependent signal transduction (PubMed:7691831). May prevent premature elimination of red blood cells (By similarity).

Alternative names

What's included?

1 x 96 Tests
Components
20X Wash Buffer
1 x 25 mL
5X Assay Diluent (Item E2)
1 x 15 mL
700X HRP-Streptavidin
1 x 200 µL
Biotinylated Human CD47 Detection Antibody
2 x 1 Vial
Human CD47 Antibody-coated ELISA Plate
1 x 1 Unit
Lyophilized Human CD47 Protein Standard
2 x 1 Vial
Stop Solution; 0.2M Sulfuric Acid
1 x 8 mL
TMB One-Step Substrate Reagent
1 x 12 mL

Recommended products

Human CD47 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human CD47 in Human in Serum, Plasma, Cell culture supernatant samples.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

0.52 - 125 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 0.52 ng/mL

Recovery

Sample specific recovery

Sample type

Serum

Average %

= 104.1

Range

85 - 118 %

Sample type

Plasma

Average %

= 104.8

Range

96 - 111 %

Sample type

Cell culture media

Average %

= 121.2

Range

110 - 133 %

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Human CD47 ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CD47 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human CD47 coated on a 96-well plate.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CD47 also referred to as integrin-associated protein carries a molecular weight of approximately 50 kDa. This protein is broadly expressed across various cell types notably on erythrocytes leukocytes and endothelial cells. Its transmembrane glycoprotein structure allows it to perform various cellular functions. CD47 interacts with specific ligands prominently SIRPα which are present on immune cells such as macrophages and dendritic cells. The engagement of CD47 with these ligands plays a major role in cellular signaling processes.

Biological function summary

CD47 functions as a "don't eat me" signal inhibiting phagocytosis by binding to SIRPα on macrophages. This protein is also important for cell adhesion and migration and can modulate interactions with integrins. While not a part of a large complex CD47 associates with various cytoskeletal and membrane proteins to maintain cellular architecture and transmission of mechanical forces. Additionally its interaction with thrombospondins influences angiogenesis and nitric oxide signaling.

Pathways

CD47 is extensively involved in the regulation of the immune response and angiogenesis. The interaction between CD47 and SIRPα plays a part in the regulation of macrophage activity impacting the immune signaling pathway. CD47 also interacts with integrins allowing it to contribute to the angiogenesis pathway which is critical in the formation of new blood vessels. These interactions help coordinate complex cellular responses and maintain tissue homeostasis.

Associated diseases and disorders

CD47 levels have associations with cancer and chronic inflammatory diseases. In cancer CD47 overexpression can allow tumor cells to evade the immune response by downregulating phagocytosis through SIRPα interaction. Therapeutically targeting CD47 using agents like anti-CD47 antibodies shows potential for enhancing anti-tumor immunity. Additionally CD47 is involved in atherosclerosis where its interaction with thrombospondin-1 contributes to disease pathogenesis by affecting nitric oxide signaling and vascular remodeling.

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1 product image

  • Sandwich ELISA - Human CD47 ELISA Kit (ab314832), expandable thumbnail

    Sandwich ELISA - Human CD47 ELISA Kit (ab314832)

    Example of standard curve using ab314832. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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