JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB324409

Human CD47 ELISA Kit - Extracellular

Be the first to review this product! Submit a review

|

(0 Publication)

Human CD47 ELISA Kit - Extracellular is a Sandwich ELISA for the measurement of Human CD47 - Extracellular in Human in Biofluids, Cell Culture Media samples.
4 Images
Sandwich ELISA - Human CD47 ELISA Kit - Extracellular (AB324409)
  • sELISA

Lab

Sandwich ELISA - Human CD47 ELISA Kit - Extracellular (AB324409)

Example of human CD47 standard curve in Sample Diluent 75BS in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human CD47 ELISA Kit - Extracellular (AB324409)
  • sELISA

Lab

Sandwich ELISA - Human CD47 ELISA Kit - Extracellular (AB324409)

Example of human CD47 standard curve in Sample Diluent 75BS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human CD47 ELISA Kit - Extracellular (AB324409)
  • sELISA

Lab

Sandwich ELISA - Human CD47 ELISA Kit - Extracellular (AB324409)

Recombinant CD47 was spiked into the following human samples and diluted in a 2-fold dilution series in Sample Diluent 75BS. Undiluted samples are 50% serum, plasma (citrate), plasma (EDTA), and plasma (heparin). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Human CD47 ELISA Kit - Extracellular (AB324409)
  • sELISA

Lab

Sandwich ELISA - Human CD47 ELISA Kit - Extracellular (AB324409)

Recombinant CD47 was spiked into the following samples and diluted in a 2-fold dilution series in Sample Diluent 75BS. Undiluted samples are 25% DMEM + 10% FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Key facts

Detection method

Colorimetric

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture media

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 46.6 pg/mL

Range

187.5 - 3000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Complementary Products

Assay Kits

AB256405

Human DAF ELISA Kit (CD55)

Recombinant|SimpleStep

Sandwich ELISA - Human DAF ELISA Kit (CD55) (AB256405)

  • 0
  • 0
Assay Kits

AB213774

Human EPCR ELISA Kit

Sandwich ELISA - Human EPCR ELISA Kit (AB213774)

  • 0
  • 0
Assay Kits

AB252351

Human Lamin B1 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Lamin B1 ELISA Kit (AB252351)

  • 0
  • 0
Assay Kits

AB283877

Human CD46 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human CD46 ELISA Kit (AB283877)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human CD47 ELISA Kit- Extracellular is a single-wash 90-min Simplestep used to quantify Human CD47 with a sensitivity of 46.6 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colormetric sandwich ELISA - 450 nm readout- works on any standard plate reader.

- Different format for different needs: 10 x 96 plates for bulk orders and 384-well for higher throughput.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
- Also available in a fully validated 384-well format.

style
left-column

Precision & recovery information

Precision

[ { "reproducibilityType": "Intra", "sample": "Plasma", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 7.1" }, { "reproducibilityType": "Inter", "sample": "Plasma", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2.2" } ]

Recovery

[ { "sample": "Serum", "range": "93 - 95 %", "average": "= 94" }, { "sample": "Citrate plasma", "range": "86 - 88 %", "average": "= 86" }, { "sample": "EDTA Plasma", "range": "83 - 98 %", "average": "= 90" }, { "sample": "Heparin Plasma", "range": "86 - 90 %", "average": "= 88" }, { "sample": "Cell culture media", "range": "116 - 119 %", "average": "= 118" } ]

style
left-column

What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab324409-10x96Tests", "assetComponentDetails": [ { "size":"10 x 1 Unit", "name":"Plate Seal", "number":"AB324409-CMP11" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB324409-CMP10" }, { "size":"10 x 20 mL", "name":"Sample Diluent 75BS", "number":"AB324409-CMP05" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB324409-CMP08" }, { "size":"10 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB324409-CMP04" }, { "size":"10 x 600 µL", "name":"10X Human CD47 Capture Antibody", "number":"AB324409-CMP01" }, { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB324409-CMP09" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB324409-CMP06" }, { "size":"20 x 1 Vial", "name":"Human CD47 Lyophilized Recombinant Protein", "number":"AB324409-CMP03" }, { "size":"10 x 600 µL", "name":"10X Human CD47 Detector Antibody", "number":"AB324409-CMP02" } ] }, "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab324409-1x384Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB324409-CMP11" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB324409-CMP04" }, { "size":"2 x 1 Vial", "name":"Human CD47 Lyophilized Recombinant Protein", "number":"AB324409-CMP03" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB324409-CMP06" }, { "size":"1 x 20 mL", "name":"Sample Diluent 75BS", "number":"AB324409-CMP05" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB324409-CMP12" }, { "size":"1 x 600 µL", "name":"10X Human CD47 Detector Antibody", "number":"AB324409-CMP02" }, { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB324409-CMP09" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB324409-CMP08" }, { "size":"1 x 600 µL", "name":"10X Human CD47 Capture Antibody", "number":"AB324409-CMP01" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab324409-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB324409-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB324409-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CD47 Capture Antibody", "number":"AB324409-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human CD47 Lyophilized Recombinant Protein", "number":"AB324409-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CD47 Detector Antibody", "number":"AB324409-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB324409-CMP10", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB324409-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB324409-CMP09", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB324409-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample Diluent 75BS", "number":"AB324409-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB324409-CMP08", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column
style
left-column

Product protocols

style
left-column

Target data

Adhesive protein that mediates cell-to-cell interactions (PubMed : 11509594, PubMed : 15383453). Acts as a receptor for thrombospondin THBS1 and as modulator of integrin signaling through the activation of heterotrimeric G proteins (PubMed : 19004835, PubMed : 7691831, PubMed : 8550562). Involved in signal transduction, cardiovascular homeostasis, inflammation, apoptosis, angiogenesis, cellular self-renewal, and immunoregulation (PubMed : 11509594, PubMed : 15383453, PubMed : 19004835, PubMed : 27742621, PubMed : 32679764, PubMed : 7691831, PubMed : 8550562). Plays a role in modulating pulmonary endothelin EDN1 signaling (PubMed : 27742621). Modulates nitrous oxide (NO) signaling, in response to THBS1, hence playing a role as a pressor agent, supporting blood pressure (By similarity). Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells (PubMed : 11509594). Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation (PubMed : 15383453). Positively modulates FAS-dependent apoptosis in T-cells, perhaps by enhancing FAS clustering (By similarity). Plays a role in suppressing angiogenesis and may be involved in metabolic dysregulation during normal aging (PubMed : 32679764). In response to THBS1, negatively modulates wound healing (By similarity). Inhibits stem cell self-renewal, in response to THBS1, probably by regulation of the stem cell transcription factors POU5F1/OCT4, SOX2, MYC/c-Myc and KLF4 (By similarity). May play a role in membrane transport and/or integrin dependent signal transduction (PubMed : 7691831). May prevent premature elimination of red blood cells (By similarity).
See full target information CD47

Alternative Names

CD47, MER6, Leukocyte surface antigen CD47, Antigenic surface determinant protein OA3, Integrin-associated protein, Protein MER6, IAP
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com