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Proteins and peptidesOur latest ELISA kit: Human Tau (phospho T217) - Intracellular
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Human CD5 ELISA Kit - Intracellular is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human CD5 - Intracellular in Cell Lysate, Tissue Extracts samples.
Alternative names=T-cell surface glycoprotein CD5, Lymphocyte antigen T1/Leu-1, LEU1, CD5
Colorimetric
Cell Lysate, Tissue Extracts
Sandwich (quantitative)
Human
10.156 - 650 pg/mL
1h 30m
= 2.9 pg/mL
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Human CD5 ELISA Kit - Intracellular is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human CD5 - Intracellular in Cell Lysate, Tissue Extracts samples.
Alternative names=T-cell surface glycoprotein CD5, Lymphocyte antigen T1/Leu-1, LEU1, CD5
Colorimetric
Cell Lysate, Tissue Extracts
Sandwich (quantitative)
Human
10.156 - 650 pg/mL
1h 30m
Pre-coated microplate (12 x 8 well strips)
= 2.9 pg/mL
105%
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Extract | n 8 | mean - | SD - | C.V. 6.5 |
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Extract | n 3 | mean - | SD - | C.V. 11.7 |
Sample type | Average % | Range |
---|---|---|
Sample type Tissue Extracts | Average % = 105 | Range 92 - 119 % |
Sample type Cell Lysate | Average % = 103 | Range 97 - 110 % |
Blue Ice
+4°C
+4°C
+4°C
Human CD5 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD5 protein in Serum, Hep Plasma, Cit Plasma, EDTA Plasma, Cell culture supernatant. Quantitate Human CD5 with 2.131 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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