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AB315046

Human CD58 ELISA Kit

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Human CD58 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CD58 with a sensitivity of 7.69 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD58, LFA3, Lymphocyte function-associated antigen 3, Ag3, Surface glycoprotein LFA-3

3 Images
Sandwich ELISA - Human CD58 ELISA Kit (AB315046)
  • sELISA

Supplier Data

Sandwich ELISA - Human CD58 ELISA Kit (AB315046)

Example of human CD58 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human CD58 ELISA Kit (AB315046)
  • sELISA

Supplier Data

Sandwich ELISA - Human CD58 ELISA Kit (AB315046)

Interpolated concentration of native CD58 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 5%, plasma (citrate) 5%, plasma (EDTA) 10%, and plasma (heparin) 5%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human CD58 ELISA Kit (AB315046)
  • sELISA

Supplier Data

Sandwich ELISA - Human CD58 ELISA Kit (AB315046)

Interpolated concentration of native CD58 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : Raji cell culture supernatant 50% and urine 40%.  The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS

Key facts

Detection method

Colorimetric

Sample types

Serum, Heparin Plasma, EDTA Plasma, Citrate plasma, Urine, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 7.69 pg/mL

Range

28.125 - 1800 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human CD58 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD58 protein in Serum, Hep Plasma, Cit Plasma, EDTA Plasma, Urine, Cell culture supernatant. Quantitate Human CD58 with 7.69 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.1" } ]
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Recovery

[ { "sample": "Serum", "range": "98 - 105 %", "average": "= 102" }, { "sample": "Heparin Plasma", "range": "93 - 97 %", "average": "= 95" }, { "sample": "EDTA Plasma", "range": "80 - 94 %", "average": "= 89" }, { "sample": "Citrate plasma", "range": "87 - 93 %", "average": "= 90" }, { "sample": "Urine", "range": "80 - 96 %", "average": "= 88" }, { "sample": "Cell culture supernatant", "range": "82 - 92 %", "average": "= 87" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD58 also known as lymphocyte function-associated antigen 3 (LFA-3) is a glycoprotein with a molecular mass of approximately 60 kDa. It is present on the surface of various cell types including antigen-presenting cells epithelial cells and some other leukocytes. This protein serves as a ligand for CD2 which is expressed on T cells and natural killer cells. Through its interaction with CD2 CD58 mediates cell adhesion and plays a role in immunological synapse formation which is essential for effective immune responses.
Biological function summary

Lymphocyte function-associated antigen 3 acts as a significant participant in the immune system. It plays a role in T cell activation and facilitates interactions between T cells and antigen-presenting cells. CD58 forms part of a complex network of cell surface proteins that enhances immune surveillance and response. Its role highlights its importance in adaptive immunity by promoting cell-to-cell communication and activation.

Pathways

CD58 participates in the cell adhesion pathways. It interacts closely with CD2 which is involved in the activation and signaling of T cells. Through this interaction CD58 plays roles in pathways like T cell receptor signaling and immune response. It enhances cell-cell adhesion helping T cells maintain their position during immune surveillance and activation.

CD58 relates to conditions such as autoimmune diseases and certain cancers. In autoimmune disorders aberrant expression or function of CD58 may contribute to inappropriate immune activation leading to tissue damage. In cancer CD58 alterations can affect tumor immune evasion influencing how the immune system recognizes and attacks cancer cells. In multiple sclerosis an autoimmune disease the interaction between CD58 and CD2 has been studied providing insights into its role in disease mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ligand of the T-lymphocyte CD2 glycoprotein. This interaction is important in mediating thymocyte interactions with thymic epithelial cells, antigen-independent and -dependent interactions of T-lymphocytes with target cells and antigen-presenting cells and the T-lymphocyte rosetting with erythrocytes. In addition, the LFA-3/CD2 interaction may prime response by both the CD2+ and LFA-3+ cells.
See full target information CD58
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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