AB263893

Human CD59 ELISA Kit

Recombinant
SimpleStep
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(3 Publications)

Human CD59 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CD59 with a sensitivity of 4.9 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD59, MIC11, MIN1, MIN2, MIN3, MSK21, CD59 glycoprotein, 1F5 antigen, 20 kDa homologous restriction factor, MAC-inhibitory protein, MEM43 antigen, Membrane attack complex inhibition factor, Membrane inhibitor of reactive lysis, Protectin, HRF-20, HRF20, MAC-IP, MACIF, MIRL

6 Images

ELISA

Supplier Data

ELISA - Human CD59 ELISA Kit (AB263893)

sELISA

Supplier Data

Sandwich ELISA - Human CD59 ELISA Kit (AB263893)

Interpolated concentrations of native CD59 in human urine and cell culture supernatant samples.

The concentrations of CD59 were measured in duplicates, interpolated from the CD59 standard curves and corrected for sample dilution. Undiluted samples are as follows : urine 1 : 3200 and HUVEC supernatant 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD59 concentration was determined to be 1328.22 ng/mL in urine and 8.97 ng/mL in HUVEC supernatant.

sELISA

Supplier Data

Sandwich ELISA - Human CD59 ELISA Kit (AB263893)

Interpolated concentrations of native CD59 in human serum and plasma samples.

The concentrations of CD59 were measured in duplicates, interpolated from the CD59 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 12.5%, plasma (citrate) 12.5%, plasma (EDTA) 12.5%, and plasma (heparin) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD59 concentration was determined to be 12.87 ng/mL in serum, 11.50 ng/mL in plasma (citrate), 9.08 ng/mL in plasma (EDTA), and 11.63 ng/mL in plasma (heparin).

sELISA

Supplier Data

Sandwich ELISA - Human CD59 ELISA Kit (AB263893)

Example of human CD59 standard curve in Sample Diluent NS + 1X Enhancer.

The CD59 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human CD59 ELISA Kit (AB263893)

6.25% Serum from ten individual healthy human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD59 concentration was determined to be 11.88 ng/mL with a range of 9.37 – 18.19 ng/mL.

sELISA

Supplier Data

Sandwich ELISA - Human CD59 ELISA Kit (AB263893)

Interpolated concentrations of native CD59 in HeLa, HUVEC, and liver cell and tissue culture extracts based on a 62.5 μg/mL, 25 μg/mL, and 31.25 μg/mL extract load, respectively.

The concentrations of CD59 were measured in duplicate and interpolated from the CD59 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD59 concentration was determined to be 2.70 ng/mL in HeLa extract, 2.70 ng/mL in HUVEC extract, and 0.77 ng/mL in liver extract.

Key facts

Detection method

Colorimetric

Sample types

Urine, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 4.9 pg/mL

Range

23.44 - 1500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human CD59 ELISA Kit ab263893 is a rapid single-wash 90-min Sandwich ELISA to measure Human CD59 in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum, tissue extracts, urine. This SimpleStep sensitivity is 4.9 pg/mL.

How the assay works

Human CD59 SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human CD59 ELISA Kit ab263893 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-CD59 antibody [EPR22394-28] - BSA and Azide free (Capture) ab267422
- Anti-CD59 antibody [EPR22394-40] - BSA and Azide free (Detector) ab267422

CD59 is a potent inhibitor of the complement membrane attack complex (MAC). It acts by binding to C8 and/or C9 of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase. The soluble form from urine retains its specific complement binding activity but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes. Glycated CD59 is found in diabetic subjects, but only at minimal levels in nondiabetic subjects. Glycated CD59 lacks MAC-inhibitory function and confers to vascular complications of diabetes. Human CD59 shares 46%, 44%, and 47% sequence homology with mouse, rat, and bovine, respectively.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.4" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.5" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "107 - 116 %", "average": "= 112" }, { "sample": "Serum", "range": "82 - 95 %", "average": "= 90" }, { "sample": "EDTA Plasma", "range": "85 - 97 %", "average": "= 92" }, { "sample": "Urine", "range": "107 - 120 %", "average": "= 111" }, { "sample": "Tissue Extracts", "range": "93 - 109 %", "average": "= 99" }, { "sample": "Heparin Plasma", "range": "84 - 109 %", "average": "= 100" }, { "sample": "Citrate plasma", "range": "80 - 117 %", "average": "= 94" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD59 also known as protectin is a protein with a molecular mass of approximately 18-25 kDa. It is widely expressed on the surface of many human cells including red blood cells and various types of leukocytes. CD59 serves as an inhibitor of the complement membrane attack complex (MAC) preventing cell lysis caused by terminal complement proteins. Through this mechanism it regulates complement activation and maintains cell integrity by halting the formation of MAC.

Biological function summary

One important feature of CD59 is its role as a glycosylphosphatidylinositol (GPI)-anchored protein involved in safeguarding cells from complement-mediated damage. It does not exist within a larger complex but functions at the cell surface to inhibit the assembly of complement components C5b-9 which form the MAC. This ability to inhibit MAC is essential in maintaining self-tissue from unintended damage during immune responses.

Pathways

CD59 participates in the regulation of the complement cascade specifically within the terminal pathway. The complement system serves as a bridge between innate and adaptive immunity contributing to processes like opsonization and cell lysis. CD59's inhibitory action directly impacts pathways that utilize terminal components such as C5b. Effective CD59 function prevents excessive complement activation ensuring that an immune response does not damage host tissues. It links closely with other complement regulatory proteins like CD55 which also mitigate complement cascade activation.

Deficiencies or malfunctions in CD59 have notable implications. Paroxysmal nocturnal hemoglobinuria (PNH) is a disorder where lack of CD59 expression on blood cells leads to their increased destruction due to unregulated complement activity. Furthermore CD59's dysfunction or inadequacy also associates with atypical hemolytic uremic syndrome (aHUS) which involves mutant complement regulatory proteins causing overactivation of the complement system. These associations highlight the importance of CD59 in both maintaining cellular health and preventing pathophysiological conditions related to complement overactivity.

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Product protocols

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Target data

Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.. The soluble form from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes.

See full target information CD59

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy. Methods & clinical development 33:101450 PubMed40231247

2025

Enhanced retinal pigment epithelial cells as a delivery vehicle for retinal disease.

Applications

Unspecified application

Species

Unspecified reactive species

Avril Reddy,Chris Greene,Yosuke Hashimoto,Anna-Sophia Kiang,Natalie Hudson,Peter Adamson,Tiago Santos-Ferreira,Matthew Campbell

International journal of molecular sciences 25: PubMed39518935

2024

Proteomic Profiling of Endothelial Cell Secretomes After Exposure to Calciprotein Particles Reveals Downregulation of Basement Membrane Assembly and Increased Release of Soluble CD59.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander Stepanov,Daria Shishkova,Victoria Markova,Yulia Markova,Alexey Frolov,Anastasia Lazebnaya,Karina Oshchepkova,Daria Perepletchikova,Daria Smirnova,Liubov Basovich,Egor Repkin,Anton Kutikhin

Bioengineering (Basel, Switzerland) 10: PubMed38002385

2023

In Vivo Assessment of Laboratory-Grown Kidney Tissue Grafts.

Applications

Unspecified application

Species

Unspecified reactive species

Tinghsien Chuang,Justin Bejar,Zhiwei Yue,Mary Slavinsky,Denise Marciano,Iain Drummond,Leif Oxburgh

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human CD59 ELISA Kit

ELISA - Human CD59 ELISA Kit (AB263893)

Sandwich ELISA - Human CD59 ELISA Kit (AB263893)

Sandwich ELISA - Human CD59 ELISA Kit (AB263893)

Sandwich ELISA - Human CD59 ELISA Kit (AB263893)

Sandwich ELISA - Human CD59 ELISA Kit (AB263893)

Sandwich ELISA - Human CD59 ELISA Kit (AB263893)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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Primary Antibodies

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