Human CD59 ELISA Kit, Chemiluminescent

• sELISA

Lab

Sandwich ELISA - Human CD59 ELISA Kit, Chemiluminescent (AB326396)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

• sELISA

Lab

Sandwich ELISA - Human CD59 ELISA Kit, Chemiluminescent (AB326396)

Dynamic range comparison between the Chemiluminescent Human CD59 SimpleStep ELISA, colorimetric Human CD59 SimpleStep ELISA (ab263893), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

• sELISA

Lab

Sandwich ELISA - Human CD59 ELISA Kit, Chemiluminescent (AB326396)

Standard curve comparison between the Chemiluminescent Human CD59 SimpleStep ELISA and colorimetric Human CD59 SimpleStep ELISA (ab263893) in Sample Diluent NS + 1X Enhancer. Background-subtracted data values (mean +/- SD) are graphed.

• sELISA

Supplier Data

Sandwich ELISA - Human CD59 ELISA Kit, Chemiluminescent (AB326396)

Interpolated concentrations of native CD59 in HeLa HUVEC and liver cell and tissue culture extracts based on a 62.5 µg/mL 25 µg/mL and 31.25 µg/mL extract load respectively.

The concentrations of CD59 were measured in duplicate and interpolated from the CD59 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean CD59 concentration was determined to be 2.70 ng/mL in HeLa extract 2.70 ng/mL in HUVEC extract and 0.77 ng/mL in liver extract.

• sELISA

Supplier Data

Sandwich ELISA - Human CD59 ELISA Kit, Chemiluminescent (AB326396)

6.25% Serum from ten individual healthy human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean CD59 concentration was determined to be 11.88 ng/mL with a range of 9.37 – 18.19 ng/mL.

• sELISA

Supplier Data

Sandwich ELISA - Human CD59 ELISA Kit, Chemiluminescent (AB326396)

Interpolated concentrations of native CD59 in human serum and plasma samples.

The concentrations of CD59 were measured in duplicates interpolated from the CD59 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 12.5% plasma (citrate) 12.5% plasma (EDTA) 12.5% and plasma (heparin) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean CD59 concentration was determined to be 12.87 ng/mL in serum 11.50 ng/mL in plasma (citrate) 9.08 ng/mL in plasma (EDTA) and 11.63 ng/mL in plasma (heparin).

• sELISA

Supplier Data

Sandwich ELISA - Human CD59 ELISA Kit, Chemiluminescent (AB326396)

Interpolated concentrations of native CD59 in human urine and cell culture supernatant samples.

The concentrations of CD59 were measured in duplicates interpolated from the CD59 standard curves and corrected for sample dilution. Undiluted samples are as follows : urine 1 : 3200 and HUVEC supernatant 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean CD59 concentration was determined to be 1328.22 ng/mL in urine and 8.97 ng/mL in HUVEC supernatant.