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AB313526

Human CD6/T12 ELISA Kit

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Human CD6/T12 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human CD6/T12 in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

CD6, T-cell differentiation antigen CD6, T12, TP120

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Sandwich ELISA - Human CD6/T12 ELISA Kit (AB313526)
  • sELISA

Supplier Data

Sandwich ELISA - Human CD6/T12 ELISA Kit (AB313526)

Example of standard curve using ab313526. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.23 ng/mL

Range

0.23 - 50 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human CD6/T12 ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CD6/T12 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human CD6/T12 coated on a 96-well plate.

This ELISA kit shows no cross-reactivity with the following cytokines tested: human ADAMTS13, Aggrecan, Angiotensinogen (SERPINA8), B7-H1 (CD274), BMPR-IA (ALK-3), BMPR-II, Cadherin-11, CD27 (TNFRSF7), Ck beta 8-1 (CCL23), CNTF, DNAM-1 (CD226), CD147 (EMMPRIN), FLRG, Follistatin-like 1, Fractalkine (CX3CL1), Galectin-1, GITR Ligand (TNFSF18), Granulysin (NKG5/LAG-2), IL-1 R3 (IL-1 R Acp), IL-15 R alpha, IL-17E (IL-25), IL32 alpha, CHL-1 (L1CAM-2), LRIG3, LRP-6, MEPE (OF45), Nectin-4, Periostin, Persephin, Renin, RGM-B, ROBO3, S100 A8, Siglec-7 (CD328), Syndecan-3, Thrombospondin 2, Thrombospondin 5 (COMP), Tie-1, ULBP-2 (ALCAN-alpha/N2DL2).

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Recovery

[ { "sample": "Serum", "range": "76 - 100 %", "average": "= 89.3" }, { "sample": "Plasma", "range": "69 - 123 %", "average": "= 79.45" }, { "sample": "Cell culture media", "range": "71 - 88 %", "average": "= 76.18" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab313526-1x96Tests", "assetComponentDetails": [ { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB313526-CMP07", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C (Item L)", "number":"AB313526-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human CD6/T12 Antibody-coated ELISA Plate", "number":"AB313526-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B (Item E)", "number":"AB313526-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human CD6/T12 Detection Antibody", "number":"AB313526-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB313526-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human CD6/T12 Protein Standard", "number":"AB313526-CMP11", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin", "number":"AB313526-CMP03", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB313526-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD6 also known as T12 is a glycoprotein expressed on T cells and some subsets of B cells. The protein has a molecular mass of approximately 105-130 kDa. CD6 is primarily located on the surface of thymocytes mature T lymphocytes and on some B cell lines. It plays an important role in the immunological synapse serving as a receptor that aids in cell-to-cell communication during immune responses. The CD6 protein is also detectable in immune tissues including the spleen and lymph nodes.
Biological function summary

CD6 functions involve T cell maturation and proliferation. The protein is part of the lymphocyte activation complex contributing to the modulation of T cell receptor (TCR) signaling. It interacts with other cell surface proteins such as CD3 and CD28 to influence the response of T cells to antigens. CD6 potentially modulates the threshold for T cell activation favoring the adjustment of immune responses to various antigens.

Pathways

CD6 participates in the signaling pathways that regulate adaptive immune responses. It is involved in the T cell receptor (TCR) signaling pathway essential for T cell activation and differentiation. CD6 interacts with proteins like LCK and ZAP-70 which are pivotal for the TCR signaling cascade. Additionally CD6's role in immune synapse formation connects it to the modulation of cytokine production and immune cell communication.

CD6 associates with autoimmune diseases and multiple sclerosis. The protein's involvement in T cell activation and immune regulation makes it a candidate for studies targeting autoimmune pathologies. Autoimmune disorders such as rheumatoid arthritis have shown links with CD6's activity as its deregulation can lead to inappropriate immune responses. Additionally CD6's interaction with proteins like CD166 has implications in the pathogenesis of these disorders indicating a potential target for therapeutic interventions.
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Product protocols

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Target data

Cell adhesion molecule that mediates cell-cell contacts and regulates T-cell responses via its interaction with ALCAM/CD166 (PubMed : 15048703, PubMed : 15294938, PubMed : 16352806, PubMed : 16914752, PubMed : 24584089, PubMed : 24945728). Contributes to signaling cascades triggered by activation of the TCR/CD3 complex (PubMed : 24584089). Functions as a costimulatory molecule; promotes T-cell activation and proliferation (PubMed : 15294938, PubMed : 16352806, PubMed : 16914752). Contributes to the formation and maturation of the immunological synapse (PubMed : 15294938, PubMed : 16352806). Functions as a calcium-dependent pattern receptor that binds and aggregates both Gram-positive and Gram-negative bacteria. Binds both lipopolysaccharide (LPS) from Gram-negative bacteria and lipoteichoic acid from Gram-positive bacteria (PubMed : 17601777). LPS binding leads to the activation of signaling cascades and down-stream MAP kinases (PubMed : 17601777). Mediates activation of the inflammatory response and the secretion of pro-inflammatory cytokines in response to LPS (PubMed : 17601777).
See full target information T-cell differentiation antigen CD6
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websiteProtocolBooklet
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Product promise

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