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AB303747

Human CD62E ELISA Kit

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Human CD62E ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CD62E with a sensitivity of 5.181 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD62E, ELAM1, SELE, E-selectin, CD62 antigen-like family member E, Endothelial leukocyte adhesion molecule 1, Leukocyte-endothelial cell adhesion molecule 2, ELAM-1, LECAM2

4 Images
Sandwich ELISA - Human CD62E ELISA Kit (AB303747)
  • sELISA

Supplier Data

Sandwich ELISA - Human CD62E ELISA Kit (AB303747)

Human CD62E competitor standard curve comparison.

Standard curve comparison between Human CD62E SimpleStep ELISA kit (ab303747) and traditional ELISA kit from leading competitor. The SimpleStep ELISA kit shows increased sensitivity.

Sandwich ELISA - Human CD62E ELISA Kit (AB303747)
  • sELISA

Supplier Data

Sandwich ELISA - Human CD62E ELISA Kit (AB303747)

Interpolated concentrations of CD62E in normal human serum donors.

Serum of 4 individual healthy human female donors (donors 1-4) and 4 individual healthy human male donors (donors 5-8) was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean CD62E concentration was determined to be 35.7 ng/mL with a range of 16.67 – 71.30 ng/mL.

Sandwich ELISA - Human CD62E ELISA Kit (AB303747)
  • sELISA

Supplier Data

Sandwich ELISA - Human CD62E ELISA Kit (AB303747)

Interpolated concentrations of human CD62E in serum, plasma (EDTA), plasma (citrate), and plasma (heparin).

Interpolated concentration of native CD62E was measured in duplicate at different sample concentrations. Undiluted samples are 2% serum, plasma (EDTA), plasma (citrate), and plasma (heparin). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human CD62E ELISA Kit (AB303747)
  • sELISA

Supplier Data

Sandwich ELISA - Human CD62E ELISA Kit (AB303747)

Example of human CD62E standard curve in Sample Diluent NS.

Example of human CD62E standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 5.181 pg/mL

Range

15.625 - 1000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human CD62E SimpleStep ELISA® kit (ab303747) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human CD62E protein in serum, plasma (EDTA), plasma (hep), plasma (cit) and cell culture media. Quantitate Human CD62E with 5.181 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.28" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.45" } ]
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Recovery

[ { "sample": "Cell culture media", "range": "90 - 99 %", "average": "= 94" }, { "sample": "Citrate plasma", "range": "84 - 102 %", "average": "= 95" }, { "sample": "EDTA Plasma", "range": "86 - 104 %", "average": "= 98" }, { "sample": "Heparin Plasma", "range": "97 - 109 %", "average": "= 104" }, { "sample": "Serum", "range": "105 - 113 %", "average": "= 109" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD62E also known as E-selectin or ELAM-1 is a cell adhesion molecule with a mass of approximately 115 kDa. It is present mainly on endothelial cells activated by cytokines. As a transmembrane glycoprotein E-selectin plays a mechanical role in mediating the tethering and rolling of leukocytes on the vascular endothelium during the inflammatory response. This function is important in directing leukocytes to sites of tissue damage or infection.
Biological function summary

E-selectin facilitates leukocyte adhesion by binding specific carbohydrate ligands on the surface of circulating immune cells. This binding is critical in the cascade of events that leads to leukocyte extravasation into tissues. E-selectin does not work in isolation rather forming part of a complex interaction with other cell adhesion molecules such as P-selectin and L-selectin. These interactions ensure precise control of cellular traffic during inflammatory responses.

Pathways

CD62E engages in the inflammatory signaling pathways including the NF-kB pathway. This pathway modulates the expression of E-selectin in response to pro-inflammatory cytokines like interleukin-1 and tumor necrosis factor-alpha. CD62E interacts with integrins on leukocytes and has downstream effects on cellular processes involved in immune response. Its cooperation with proteins like ICAM-1 and VCAM-1 further integrates it into a network of adhesion molecules maintaining vascular stability and immune surveillance.

E-selectin expression is highly relevant in inflammatory diseases such as rheumatoid arthritis and atherosclerosis. These disorders involve chronic inflammation where the persistent activation of endothelial cells and overexpression of E-selectin contribute to pathology. The link with ICAM-1 in these settings suggests a mutual regulation between cell adhesion molecules enhancing leukocyte recruitment to inflamed tissues. This makes E-selectin not only a marker of endothelial activation but also a potential therapeutic target for modulating leukocyte adhesion in inflammatory diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis.
See full target information SELE
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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