JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB45917

Human CD62L ELISA Kit (Selectin L)

Be the first to review this product! Submit a review

|

(1 Publication)

Human CD62L ELISA Kit (Selectin L) is a Sandwich ELISA for the measurement of Human CD62L (Selectin L) in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

CD62L, LNHR, LYAM1, SELL, L-selectin, CD62 antigen-like family member L, Leukocyte adhesion molecule 1, Leukocyte surface antigen Leu-8, Leukocyte-endothelial cell adhesion molecule 1, Lymph node homing receptor, TQ1, gp90-MEL, LAM-1, LECAM1

1 Images
Sandwich ELISA - Human CD62L ELISA Kit (Selectin L) (AB45917)
  • sELISA

Supplier Data

Sandwich ELISA - Human CD62L ELISA Kit (Selectin L) (AB45917)

Representative Standard Curve using ab45917

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 1 ng/mL

Range

1.9 - 60 ng/mL

Assay time

1h 45m

Assay Platform

Microplate

style
left-column

Complementary Products

Assay Kits

AB254501

Human Complement C2 ELISA Kit

Recombinant|SimpleStep

ELISA - Human Complement C2 ELISA Kit (AB254501)

  • 0
  • 0
Assay Kits

AB284615

Human Complement Factor I ELISA Kit

Recombinant|SimpleStep

ELISA - Human Complement Factor I ELISA Kit (AB284615)

  • 0
  • 0
Assay Kits

AB300318

Human Factor B ELISA Kit

Recombinant|SimpleStep

ELISA - Human Factor B ELISA Kit (AB300318)

  • 4
  • 1
Assay Kits

AB270215

Human Gelsolin ELISA Kit

Recombinant|SimpleStep

ELISA - Human Gelsolin ELISA Kit (AB270215)

  • 5
  • 1
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Abcam's CD62L (Selectin L) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of CD62L in Human serum, plasma, buffered solutions and cell culture medium.

A monoclonal antibody specific for CD62L has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD62L concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for CD62L are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of CD62L present in the samples.

This kit will recognize both endogenous and recombinant Human CD62L.

style
left-column

Precision

[ { "reproducibilityType": "Intra", "sample": "Sample A", "replicates": 15, "mean": "28 ng/mL", "standardDeviation": "1.3", "coefficientOfVariability": "4.6" }, { "reproducibilityType": "Intra", "sample": "Sample B", "replicates": 19, "mean": "9 ng/mL", "standardDeviation": "0.5", "coefficientOfVariability": "5.5" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab45917-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"HRP Diluent", "number":"AB45917-CMP03" }, { "size":"2 x 1 Vial", "name":"CD62L Standard", "number":"AB45917-CMP09" }, { "size":"2 x 1 Vial", "name":"CD62L Control", "number":"AB45917-CMP07" }, { "size":"1 x 15 mL", "name":"10X Standard Diluent Buffer", "number":"AB45917-CMP06" }, { "size":"1 x 0.4 mL", "name":"Biotinylated anti-CD62L antibody", "number":"AB45917-CMP11" }, { "size":"1 x 7 mL", "name":"Biotinylated Antibody Diluent", "number":"AB45917-CMP01" }, { "size":"1 x 10 mL", "name":"200X Wash Buffer", "number":"AB45917-CMP05" }, { "size":"1 x 1 Unit", "name":"CD62L Microplate (12 x 8 well strips)", "number":"AB45917-CMP08" }, { "size":"2 x 5 µL", "name":"Streptavidin-HRP", "number":"AB45917-CMP04" }, { "size":"1 x 11 mL", "name":"Stop Reagent", "number":"AB45917-CMP02" }, { "size":"1 x 11 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB45917-CMP10" } ] }, "2x96Tests": { "sellingSize": "2 x 96 Tests", "publicAssetCode":"ab45917-2x96Tests", "assetComponentDetails": [ { "size":"1 x 23 mL", "name":"HRP Diluent", "number":"AB45917-CMP03", "productcode":"" }, { "size":"4 x 1 Vial", "name":"CD62L Standard", "number":"AB45917-CMP09", "productcode":"" }, { "size":"4 x 1 Vial", "name":"CD62L Control", "number":"AB45917-CMP07", "productcode":"" }, { "size":"2 x 1 Unit", "name":"CD62L Microplate (12 x 8 well strips)", "number":"AB45917-CMP08", "productcode":"" }, { "size":"1 x 24 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB45917-CMP10", "productcode":"" }, { "size":"1 x 25 mL", "name":"10X Standard Diluent Buffer", "number":"AB45917-CMP06", "productcode":"" }, { "size":"2 x 0.4 mL", "name":"Biotinylated anti-CD62L antibody", "number":"AB45917-CMP11", "productcode":"" }, { "size":"2 x 10 mL", "name":"200X Wash Buffer", "number":"AB45917-CMP05", "productcode":"" }, { "size":"1 x 13 mL", "name":"Biotinylated Antibody Diluent", "number":"AB45917-CMP01", "productcode":"" }, { "size":"2 x 11 mL", "name":"Stop Reagent", "number":"AB45917-CMP02", "productcode":"" }, { "size":"4 x 5 µL", "name":"Streptavidin-HRP", "number":"AB45917-CMP04", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD62L also known as L-selectin is a cell adhesion molecule with a mass of approximately 74 kDa. It is part of the L-selectin family and is found on the surface of leukocytes including T cells B cells neutrophils and monocytes. Mouse models frequently study CD62L under the name "MEL-14". CD62L plays an important role in mediating the adhesion and rolling of leukocytes on blood vessel endothelial cells during the immune response. Its structure allows it to interact specifically with carbohydrates on endothelial cells facilitating the initial steps of leukocyte extravasation into tissues.
Biological function summary

CD62L is involved in the migration of leukocytes into lymphoid tissues and sites of inflammation. It is not known to be part of a larger protein complex; rather it functions independently on the cell membrane. CD62L also assists in the homing of naive lymphocytes to peripheral lymph nodes. The molecule regulates how immune cells enter tissues through high endothelial venules acting as a critical entry point for leukocyte trafficking.

Pathways

CD62L contributes significantly to the immune trafficking pathway and leukocyte adhesion cascade. It works closely with other adhesion molecules like CD44 and CD54 (ICAM-1) which are important within the context of cellular responses during inflammation. CD62L's function in these pathways ensures that immune cells are properly directed to areas where they are needed most whether that is secondary lymphoid organs or inflamed tissues.

CD62L expression and function have been associated with inflammatory conditions and certain immune-related pathologies such as multiple sclerosis and rheumatoid arthritis. Altered CD62L expression can affect lymphocyte homing potentially playing a role in the pathogenesis of these conditions. The role of CD62L is also linked to proteins like CD44 which can modulate cell interactions and have overlapping functions in disease mechanisms influencing disease progression or resolution.
style
left-column

Product protocols

style
left-column

Target data

Calcium-dependent lectin that mediates cell adhesion by binding to glycoproteins on neighboring cells (PubMed : 12403782, PubMed : 28011641, PubMed : 28489325). Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia (PubMed : 12403782, PubMed : 28011641).
See full target information SELL
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PLoS computational biology 6: PubMed20885780

2010

Differentially expressed RNA from public microarray data identifies serum protein biomarkers for cross-organ transplant rejection and other conditions.

Applications

ELISA

Species

Human

Rong Chen,Tara K Sigdel,Li Li,Neeraja Kambham,Joel T Dudley,Szu-Chuan Hsieh,R Bryan Klassen,Amery Chen,Tuyen Caohuu,Alexander A Morgan,Hannah A Valantine,Kiran K Khush,Minnie M Sarwal,Atul J Butte
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com