AB275099

Human CD63 ELISA Kit

Recombinant
SimpleStep
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(2 Publications)

Human CD63 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CD63 with a sensitivity of 33 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD63, MLA1, TSPAN30, CD63 antigen, Granulophysin, Lysosomal-associated membrane protein 3, Lysosome integral membrane protein 1, Melanoma-associated antigen ME491, OMA81H, Ocular melanoma-associated antigen, Tetraspanin-30, LAMP-3, Limp1, Tspan-30

5 Images

ELISA

Supplier Data

ELISA - Human CD63 ELISA Kit (AB275099)

sELISA

Supplier Data

Sandwich ELISA - Human CD63 ELISA Kit (AB275099)

Serum from eight individual healthy CD63 female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD63 concentration was determined to be 252 pg/mL with a range of 103 – 408 pg/mL.

sELISA

Lab

Sandwich ELISA - Human CD63 ELISA Kit (AB275099)

Example of human CD63 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human CD63 ELISA Kit (AB275099)

Interpolated concentration of native Human CD63 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : Purified MCF-7 exosome standards (ab239691) 25 µg/mL (dilutions made into Sample Diluent NS) and denatured MCF-7 exosome standards (ab239691) 2.5 µg/mL (dilutions made in Sample Diluent NS + Enhancer). The interpolated dilution factor corrected values as a ratio of µg/mL exosome load are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Human CD63 ELISA Kit (AB275099)

Example of human CD63 standard curve in Sample Diluent NS.

The CD63 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Serum, EDTA Plasma, Exosome

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 33 pg/mL

Range

46.9 - 3000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human CD63 ELISA Kit ab275099 is a rapid single-wash 90-min Sandwich ELISA to measure Human CD63 in cell culture supernatant, EDTA plasma, serum. This SimpleStep sensitivity is 33 pg/mL.

How the assay works

Human CD63 SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human CD63 ELISA Kit ab275099 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Save your precious samples

Our 384-well Human CD63 ELISA Kit only requires a maximum of 12.5µL sample

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-CD63 antibody [EPR22458-69] - BSA and Azide free (Capture) ab275273
- Anti-CD63 antibody [EPR22458-293] - BSA and Azide free (Detector) ab275273

Human CD63 antigen, also referred to as Granulophysin, Lysomal-associated membrane protein 3 (LAMP-3), Tetraspanin-30, and Ocular melanoma-associated antigen, is a membrane bound protein encoded by the gene CD63. CD63 belongs to a group of proteins known as the tetraspanin family, which are characterized by the presence of four hydrophobic domains and are found to mediate signal transduction events for the regulation of cell development, activation, growth, and motility. Deficiency of this protein is associated with Hermansky-Pudlak Syndrome, which is an autosomal disorder.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.7" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 7, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.4" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "96 - 98 %", "average": "= 97" }, { "sample": "Serum", "range": "94 - 101 %", "average": "= 97" }, { "sample": "EDTA Plasma", "range": "90 - 96 %", "average": "= 94" } ]

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What's included?

{ "values": { "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab275099-1x384Tests", "assetComponentDetails": [ { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB275099-CMP06" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB275099-CMP11" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB275099-CMP12" }, { "size":"2 x 1 Vial", "name":"Human CD63 Lyophilized Recombinant Protein", "number":"AB275099-CMP08" }, { "size":"1 x 600 µL", "name":"10X Human CD63 Capture Antibody", "number":"AB275099-CMP01" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB275099-CMP10" }, { "size":"1 x 600 µL", "name":"10X Human CD63 Detector Antibody", "number":"AB275099-CMP02" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB275099-CMP09" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB275099-CMP07" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB275099-CMP13" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab275099-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB275099-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB275099-CMP12", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CD63 Capture Antibody", "number":"AB275099-CMP01", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB275099-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB275099-CMP11", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human CD63 Lyophilized Recombinant Protein", "number":"AB275099-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CD63 Detector Antibody", "number":"AB275099-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB275099-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB275099-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB275099-CMP07", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD63 is a protein commonly known as LAMP-3 a member of the tetraspanin family with a molecular weight of approximately 25-65 kDa depending on post-translational modifications such as glycosylation. It resides mainly on the membrane of intracellular vesicles such as lysosomes and platelets. CD63 also appears on the surface of activated cells particularly in immune cells. Researchers often use CD63 as a marker in assays including CD63 western blot techniques due to its distinct expression patterns.

Biological function summary

CD63 participates in various cellular processes including membrane trafficking cell adhesion and signal transduction. It associates with integrins and other tetraspanins forming complexes that influence cellular adhesion and migration. Through its role in exosome formation CD63 contributes significantly to intercellular communication by facilitating the transfer of proteins lipids and RNA between cells.

Pathways

CD63 plays a role in pathways linked to cell proliferation and migration and immune response regulation. Within the integrin-mediated signaling pathway CD63 interacts with proteins such as integrins to promote cell adhesion and migration. CD63 also links to vesicle trafficking pathways collaborating with proteins involved in exocytosis and endocytosis which affect cell membrane restructuring and intracellular transport.

CD63 shows connections with cancer and immune system disorders. Its role in promoting cell adhesion and migration links it with cancer metastasis including melanoma and breast cancer where CD63 expression levels often rise. Additionally CD63 associates with allergic reactions due to its involvement in histamine release where it impacts immune proteins like FcεRI which participate in allergy pathogenesis.

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Product protocols

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Target data

The protein expressed by gene CD63 functions as a cell surface receptor for TIMP1 and is involved in the activation of various cellular signaling cascades. It plays a role in the activation of ITGB1 and integrin signaling, which leads to the activation of AKT, FAK/PTK2, and MAP kinases, promoting cell survival, actin cytoskeleton reorganization, and cell adhesion, spreading, and migration through the activation of AKT and FAK/PTK2. Additionally, it participates in VEGFA signaling by regulating the internalization of KDR/VEGFR2. CD63 is involved in intracellular vesicular transport processes and is essential for the normal trafficking of the PMEL luminal domain, crucial for melanocyte development and maturation. It also contributes to leukocyte adhesion onto endothelial cells by regulating SELP trafficking. CD63 may play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in response to other stimuli. This supplementary information is collated from multiple sources and compiled automatically.

See full target information CD63

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of extracellular vesicles 12:e12348 PubMed37489102

2023

Label-free discrimination of extracellular vesicles from large lipoproteins.

Applications

Unspecified application

Species

Unspecified reactive species

Anna D Kashkanova,Martin Blessing,Marie Reischke,Jan-Ole Baur,Andreas S Baur,Vahid Sandoghdar,Jan Van Deun

Journal of leukocyte biology 112:1633-1648 PubMed36040107

2022

Single-cell phenotypic profiling to identify a set of immune cell protein biomarkers for relapsed and refractory diffuse large B cell lymphoma: A single-center study.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Shi,Weidong Ding,Weiying Gu,Yangling Shen,Haiqian Li,Zhuojun Zheng,Xiao Zheng,Yan Liu,Yun Ling

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human CD63 ELISA Kit

ELISA - Human CD63 ELISA Kit (AB275099)

Sandwich ELISA - Human CD63 ELISA Kit (AB275099)

Sandwich ELISA - Human CD63 ELISA Kit (AB275099)

Sandwich ELISA - Human CD63 ELISA Kit (AB275099)

Sandwich ELISA - Human CD63 ELISA Kit (AB275099)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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