JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB282869

Human CD66b ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Human CD66b ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CD66b with a sensitivity of 3.93 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD66b, CGM6, CEACAM8, Cell adhesion molecule CEACAM8, CD67 antigen, Carcinoembryonic antigen CGM6, Carcinoembryonic antigen-related cell adhesion molecule 8, Non-specific cross-reacting antigen NCA-95, CEA cell adhesion molecule 8

4 Images
Sandwich ELISA - Human CD66b ELISA Kit (AB282869)
  • sELISA

Supplier Data

Sandwich ELISA - Human CD66b ELISA Kit (AB282869)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD66b concentration was determined to be 717.64 pg/mL with a range of 356.71 – 1,292.71 pg/mL.

Sandwich ELISA - Human CD66b ELISA Kit (AB282869)
  • sELISA

Supplier Data

Sandwich ELISA - Human CD66b ELISA Kit (AB282869)

Interpolated concentrations of native CD66b in human PBMC supernatant samples.

Stimulated samples were treated with 1.5% PHA-M for 46 hours. The concentrations of CD66b were measured in duplicates, interpolated from the CD66b standard curves and corrected for sample dilution. Undiluted samples are as follows : stimulated PBMC supernatant 100% and unstimulated PBMC supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD66b concentration was determined to be 263.55 pg/mL in stimulated PBMC supernatant and 151.03 pg/mL in unstimulated PBMC supernatant.

Sandwich ELISA - Human CD66b ELISA Kit (AB282869)
  • sELISA

Supplier Data

Sandwich ELISA - Human CD66b ELISA Kit (AB282869)

Example of human CD66b standard curve in Sample Diluent NS.

The CD66b standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human CD66b ELISA Kit (AB282869)
  • sELISA

Supplier Data

Sandwich ELISA - Human CD66b ELISA Kit (AB282869)

Interpolated concentrations of native CD66b in human serum and plasma samples.

The concentrations of CD66b were measured in duplicates, interpolated from the CD66b standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (heparin) 50%, and plasma (EDTA) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD66b concentration was determined to be 802.67 pg/mL in serum, 833.01 pg/mL in plasma (citrate), 1,208.45 pg/mL in plasma (heparin), and 531.15 pg/mL in plasma (EDTA).

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 3.93 pg/mL

Range

21.88 - 1400 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human CD66b ELISA kit (ab282869) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human CD66b protein in human serum, plasma, and cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate CD66b with 3.93 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

CD66b is a cell surface glycoprotein involved in cell adhesion, cell migration and pathogen binding. It is a member of the CEA superfamily of immunoglobulins. CD66b is exclusively expressed on granulocytes. Soluble CD66b is released in response to bacterial DNA.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.3" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5" } ]
style
left-column

Recovery

[ { "sample": "Cell culture supernatant", "range": "104 - 108 %", "average": "= 106" }, { "sample": "Serum", "range": "87 - 111 %", "average": "= 98" }, { "sample": "EDTA Plasma", "range": "89 - 107 %", "average": "= 99" }, { "sample": "Heparin Plasma", "range": "95 - 104 %", "average": "= 99" }, { "sample": "Citrate plasma", "range": "90 - 103 %", "average": "= 95" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab282869-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB282869-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB282869-CMP12", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB282869-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human CD66b Lyophilized Recombinant Protein", "number":"AB282869-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB282869-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CD66b Capture Antibody", "number":"AB282869-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB282869-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB282869-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CD66b Detector Antibody", "number":"AB282869-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB282869-CMP05", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD66b also known as carcinoembryonic antigen-related cell adhesion molecule 8 (CEACAM8) is a glycoprotein with a molecular weight around 95 kDa. It is primarily expressed on the surface of granulocytes specifically on neutrophils—its expression on these cells makes it a useful marker in distinguishing stages of granulocyte maturation. The protein localizes to the cell membrane and serves as a cell adhesion molecule playing roles in intercellular interactions. Researchers often use CD66b-specific antibodies such as CD66b FITC for detecting and analyzing neutrophil populations commonly in flow cytometry applications.
Biological function summary

CD66b contributes to immune responses by facilitating leukocyte activation and migration during inflammation. It does not form part of a multi-protein complex but interacts with other cellular components. CD66b promotes neutrophil adhesion and transmigration to sites of infection or injury supporting their role in innate immunity. Additionally it participates in signaling pathways that influence cell survival proliferation and apoptosis in response to environmental cues.

Pathways

CD66b operates within neutrophil-related processes like the immune response and cell adhesion pathways. In the immune response pathway it collaborates with integrins to assist neutrophil extravasation through the endothelium. During inflammatory responses CD66b interaction with integrins and other adhesion molecules influences the intensity and duration of activity at inflammation sites. This interaction highlights its involvement with proteins such as ICAM-1 in mediating firm adhesion necessary for neutrophil migration.

CD66b levels and activity correlate with conditions involving inflammation such as chronic obstructive pulmonary disease (COPD) and rheumatoid arthritis. During COPD the altered expression and regulation of CD66b contribute to neutrophil-driven inflammation exacerbating disease symptoms. Similarly rheumatoid arthritis involves inflammatory pathways where aberrant CD66b activity enhances synovial inflammation often linked with other inflammatory markers like TNF-alpha. Understanding CD66b's role in such diseases aids the development of therapeutic strategies targeting neutrophil-mediated pathology.
style
left-column

Product protocols

style
left-column

Target data

Cell surface glycoprotein that plays a role in cell adhesion in a calcium-independent manner (PubMed : 11590190, PubMed : 2022629, PubMed : 8776764). Mediates heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6 (PubMed : 11590190, PubMed : 2022629, PubMed : 8776764). Heterophilic interaction with CEACAM8 occurs in activated neutrophils (PubMed : 8776764).
See full target information CEACAM8
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Primary Antibodies

AB300122

Anti-CD66b antibody [EPR25354-2]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD66b antibody [EPR25354-2] (AB300122)

  • 5
  • 1
Assay Kits

AB315307

Human NCAM1 ELISA Kit

SimpleStep

Sandwich ELISA - Human NCAM1 ELISA Kit (AB315307)

  • 0
  • 0
Assay Kits

AB222510

Human VEGF ELISA Kit

SimpleStep

ELISA - Human VEGF ELISA Kit (AB222510)

  • 5
  • 1
Assay Kits

AB224881

Human TREM2 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human TREM2 ELISA Kit (AB224881)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com