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AB326010

Human CD66b ELISA Kit, Chemiluminescent

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Human CD66b ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human CD66b with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 5.94 - 13000 pg/ml with a sensitivity of 2.19 pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending

View Alternative Names

CD66b, CGM6, CEACAM8, Cell adhesion molecule CEACAM8, CD67 antigen, Carcinoembryonic antigen CGM6, Carcinoembryonic antigen-related cell adhesion molecule 8, Non-specific cross-reacting antigen NCA-95, CEA cell adhesion molecule 8

6 Images
Sandwich ELISA - Human CD66b ELISA Kit, Chemiluminescent (AB326010)
  • sELISA

Lab

Sandwich ELISA - Human CD66b ELISA Kit, Chemiluminescent (AB326010)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human CD66b ELISA Kit, Chemiluminescent (AB326010)
  • sELISA

Lab

Sandwich ELISA - Human CD66b ELISA Kit, Chemiluminescent (AB326010)

Interpolated concentrations of native CD66b in human serum and plasma samples. The concentrations of CD66b were measured in duplicates, interpolated from the CD66b standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (heparin) 50%, and plasma (EDTA) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD66b concentration was determined to be 802.67 pg/mL in serum, 833.01 pg/mL in plasma (citrate), 1,208.45 pg/mL in plasma (heparin), and 531.15 pg/mL in plasma (EDTA).

Sandwich ELISA - Human CD66b ELISA Kit, Chemiluminescent (AB326010)
  • sELISA

Lab

Sandwich ELISA - Human CD66b ELISA Kit, Chemiluminescent (AB326010)

Dynamic range comparison between the Chemiluminescent Human CD66b SimpleStep ELISA, colorimetric Human CD66b SimpleStep ELISA (ab282869), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Sandwich ELISA - Human CD66b ELISA Kit, Chemiluminescent (AB326010)
  • sELISA

Lab

Sandwich ELISA - Human CD66b ELISA Kit, Chemiluminescent (AB326010)

Standard curve comparison between the Chemiluminescent Human CD66b SimpleStep ELISA and colorimetric Human CD66b SimpleStep ELISA (ab282869) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human CD66b ELISA Kit, Chemiluminescent (AB326010)
  • sELISA

Lab

Sandwich ELISA - Human CD66b ELISA Kit, Chemiluminescent (AB326010)

Interpolated concentrations of native CD66b in human PBMC supernatant samples. Stimulated samples were treated with 1.5% PHA-M for 46 hours. The concentrations of CD66b were measured in duplicates, interpolated from the CD66b standard curves and corrected for sample dilution. Undiluted samples are as follows : stimulated PBMC supernatant 100% and unstimulated PBMC supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD66b concentration was determined to be 263.55 pg/mL in stimulated PBMC supernatant and 151.03 pg/mL in unstimulated PBMC supernatant.

Sandwich ELISA - Human CD66b ELISA Kit, Chemiluminescent (AB326010)
  • sELISA

Supplier Data

Sandwich ELISA - Human CD66b ELISA Kit, Chemiluminescent (AB326010)

Serum from ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD66b concentration was determined to be 717.64 pg/mL with a range of 356.71 – 1,292.71 pg/mL

Key facts

Detection method

Luminescent

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 2.19 pg/mL

Range

5.94 - 13000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

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Product details

Human CD66b ELISA Kit, Chemiluminescent (ab326010) is a rapid single-wash sandwich 55-min ELISA to measure Human CD66b in Serum, Citrate Plasma, EDTA Plasma, Heparin Plasma, Cell Culture Supernatant with a sensitivity of 2.19 pg/mL.

How the assay works

Human CD66b ELISA Kit, Chemiluminescent (ab326010) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human CD66b (ab326010) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-CEACAM8 antibody [EPR23955-20] - BSA and Aside free (Capture) ab281146
- Anti-CEACAM8 antibody [EPR23955-130] - BSA and Aside free (Detector) ab280996
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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 5" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 6.3" } ]
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Recovery

[ { "sample": "Serum", "range": "87 - 111 %", "average": "= 98" }, { "sample": "Citrate plasma", "range": "90 - 103 %", "average": "= 95" }, { "sample": "EDTA Plasma", "range": "95 - 104 %", "average": "= 99" }, { "sample": "Heparin Plasma", "range": "89 - 107 %", "average": "= 99" }, { "sample": "Cell culture supernatant", "range": "104 - 108 %", "average": "= 106" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Cell surface glycoprotein that plays a role in cell adhesion in a calcium-independent manner (PubMed : 11590190, PubMed : 2022629, PubMed : 8776764). Mediates heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6 (PubMed : 11590190, PubMed : 2022629, PubMed : 8776764). Heterophilic interaction with CEACAM8 occurs in activated neutrophils (PubMed : 8776764).
See full target information CEACAM8
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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