Human CD68 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CD68 with a sensitivity of 7.6 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Cell culture supernatant, Serum, Cell Lysate, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 62.5 - 4000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 7.6 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
Alternative names
CD68, Macrosialin, Gp110
What's included?
Recommended products
Human CD68 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CD68 with a sensitivity of 7.6 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Cell culture supernatant, Serum, Cell Lysate, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 62.5 - 4000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 7.6 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 6.6 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 9.4 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 104 | Range 98 - 114 % |
Sample type Serum | Average % = 113 | Range 109 - 118 % |
Sample type EDTA Plasma | Average % = 110 | Range 107 - 114 % |
Sample type Heparin Plasma | Average % = 98 | Range 89 - 110 % |
Sample type Citrate plasma | Average % = 100 | Range 92 - 110 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human CD68 ELISA kit (ab287167) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human CD68 protein in human serum, plasma, cell culture supernatant and cell extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CD68 with 7.60 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD68 is a glycoprotein that functions as a scavenger receptor. It plays an important role in the regulation of cellular debris and apoptotic cell clearance. Researchers often refer to it as KP1 or macrosialin in the literature. CD68 with a molecular weight of approximately 110 kDa is highly expressed in macrophages and monocytes. The mucin-like structure of CD68 allows it to be heavily glycosylated which aids in its function. Researchers detect CD68 in tissues through methods like immunohistochemistry (IHC) immunofluorescence and CD68 staining and it is widely used as a macrophage marker in research.
- Biological function summary
CD68 facilitates functions associated with the innate immune response. It serves as an important part of macrophages which contribute to immune surveillance and tissue homeostasis. Though it primarily operates on its own in some contexts CD68 might assist in forming complexes with other receptors to modulate phagocytic activity. This target is significant in atherosclerotic plaque stability as macrophages engulf lipids and cell debris through processes facilitated by CD68.
- Pathways
CD68 is involved in pathways connected to inflammation and phagocytosis. The CD68 protein works closely with other scavenger receptors like CD36 to mediate uptake of oxidized low-density lipoproteins (oxLDL) in the lipid metabolism pathway. Additionally CD68 engagement can influence the toll-like receptor (TLR) signaling pathways thereby linking innate immunity and inflammatory responses critical for host defense and disease progression.
- Associated diseases and disorders
CD68 is associated with atherosclerosis and multiple sclerosis. In atherosclerosis its role becomes evident as it accumulates in macrophages within the plaques making it a marker of disease severity. In multiple sclerosis CD68 expression in macrophages and microglia indicates active demyelination and inflammation. It also relates to proteins such as CD36 in atherosclerosis where both mediate the uptake of modified LDL contributing to foam cell formation in plaques.
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5 product images
Sandwich ELISA - Human CD68 ELISA Kit (ab287167)
Example of human CD68 standard curve in Sample Diluent NS.
Example of human CD68 standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human CD68 ELISA Kit (ab287167)
Example of human CD68 standard curve in 1X Cell Extraction Buffer PTR.
Example of human CD68 standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human CD68 ELISA Kit (ab287167)
Interpolated concentrations of human CD68 in serum, plasma(citrate), plasma (EDTA), and plasma (heparin).
Interpolated concentration of native CD68 was measured in duplicate at different sample concentrations. Undiluted samples are as follows: serum 50%, plasma (citrate) 100%, plasma (EDTA) 100%, and plasma (heparin)100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human CD68 ELISA Kit (ab287167)
Interpolated concentrations of spiked human CD68 in 10F RPMI.
Recombinant CD68 was spiked into the following species samples and diluted in a 2-fold dilution series in Sample Diluent NS. Undiluted samples are 100% 10F RPMI. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Sandwich ELISA - Human CD68 ELISA Kit (ab287167)
Interpolated concentrations of human CD68 in U937 extract, and THP-1 extract.
Interpolated concentration of native CD68 was measured in duplicate at different sample concentrations. Undiluted samples are as follows: U937 extract 100 ug/mL, and THP-1 extract ug/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.
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