Human CD70 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human CD70 in Human in Plasma, Cell culture media, Serum samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture media, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.287 - 70 ng/mL
- Sensitivity
- = 0.3 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Expressed at the plasma membrane of B cells, it is the ligand of the CD27 receptor which is specifically expressed at the surface of T cells (PubMed:28011863, PubMed:28011864, PubMed:8387892). The CD70-CD27 signaling pathway mediates antigen-specific T cell activation and expansion which in turn provides immune surveillance of B cells (PubMed:28011863, PubMed:28011864).
Alternative names
CD70, CD27L, CD27LG, TNFSF7, CD70 antigen, CD27 ligand, Tumor necrosis factor ligand superfamily member 7, CD27-L
What's included?
Recommended products
Human CD70 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human CD70 in Human in Plasma, Cell culture media, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture media, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.287 - 70 ng/mL
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 0.3 ng/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 10 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 12 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 117 | Range 93 - 145 % |
Sample type Plasma | Average % = 96 | Range 69 - 125 % |
Sample type Cell culture media | Average % = 87 | Range 68 - 123 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Human CD70 ELISA Kit is designed for the quantitative determination of CD70 in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for human CD70 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD70 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human CD70 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD70 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD70 also known as CD27 ligand or CD27L is a type II transmembrane protein with a molecular mass of approximately 30 kDa. It belongs to the tumor necrosis factor (TNF) superfamily and is encoded by the CD70 gene. The expression of CD70 occurs mainly on activated T and B cells dendritic cells and some NK cells but not on resting lymphocytes. In addition CD70 expression is observed in certain malignancies and inflammatory conditions.
- Biological function summary
CD70 plays an essential role in the regulation of immune responses. It does this by binding to its receptor CD27 forming a receptor-ligand complex that triggers signaling pathways vital for T cell proliferation survival and differentiation. This interaction also influences the production of immunoglobulins by B cells. CD70-CD27 signaling is particularly important in shaping adaptive immunity enhancing the body's ability to respond to pathogens effectively.
- Pathways
CD70 is integral in the activation of the NF-κB and MAPK pathways. These pathways are activated upon the interaction of CD70 with CD27 promoting cell survival proliferation and pro-inflammatory cytokine production. Other proteins related to CD70 through these pathways include TRAF2 and TRAF3 which are essential for downstream signaling events that modulate immune responses.
- Associated diseases and disorders
CD70 has been implicated in autoimmune diseases and certain cancers. For instance it plays a role in the pathogenesis of lupus by contributing to abnormal immune activation. Overexpression of CD70 is also associated with various cancers such as renal cell carcinoma where it can support tumor cell growth and immune evasion. Through these conditions CD70 interacts with proteins like BCL-2 in cancer affecting cell survival pathways while its interaction with CD27 influences the immune deregulation seen in lupus.
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1 product image
Sandwich ELISA - Human CD70 ELISA Kit (ab267612)
Human CD70 ELISA kit (ab267612) Standard curve.
Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
CD27L is an alternative name for CD70.
Downloads
Product protocols
- Visit the general protocols
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