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AB267612

Human CD70 ELISA Kit

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Human CD70 ELISA Kit is a sandwich ELISA designed to quantify Human CD70 with a sensitivity of 0.3 ng/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 0.287 - 70 ng/mL

View Alternative Names

CD70, CD27L, CD27LG, TNFSF7, CD70 antigen, CD27 ligand, Tumor necrosis factor ligand superfamily member 7, CD27-L

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Sandwich ELISA - Human CD70 ELISA Kit (AB267612)
  • sELISA

Supplier Data

Sandwich ELISA - Human CD70 ELISA Kit (AB267612)

Human CD70 ELISA kit (ab267612) Standard curve.

Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

CD27L is an alternative name for CD70.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture media, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.3 ng/mL

Range

0.287 - 70 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human CD70 ELISA Kit ab267612 is a sandwich ELISA to measure Human CD70 in serum, plasma, cell culture media with a sensitivity of 0.3 ng/ml.

How the assay works

This assay employs an antibody specific for human CD70 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD70 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human CD70 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD70 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Human CD70 ELISA Kit ab267612 protocol summary

1. Add standard or sample to each well used. Incubate at room temperature
2. Wash and add prepared biotinylated antibody to each well. Incubate at room temperature.
3. Wash and add prepared streptavidin solution. Incubate at room temperature.
4. Add TMB Development Solution to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read at 450nm immediately.
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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "93 - 145 %", "average": "= 117" }, { "sample": "Plasma", "range": "69 - 125 %", "average": "= 96" }, { "sample": "Cell culture media", "range": "68 - 123 %", "average": "= 87" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab267612-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB267612-CMP08", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB267612-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human CD70 coated Microplate (12 x 8 wells)", "number":"AB267612-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB267612-CMP06", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB267612-CMP02", "productcode":"" }, { "size":"1 x 200 µL", "name":"100X HRP-Streptavidin Concentrate", "number":"AB267612-CMP07", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C", "number":"AB267612-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human CD70 Detection Antibody", "number":"AB267612-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human CD70 Standard (Lyophilized)", "number":"AB267612-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD70 also known as CD27 ligand or CD27L is a type II transmembrane protein with a molecular mass of approximately 30 kDa. It belongs to the tumor necrosis factor (TNF) superfamily and is encoded by the CD70 gene. The expression of CD70 occurs mainly on activated T and B cells dendritic cells and some NK cells but not on resting lymphocytes. In addition CD70 expression is observed in certain malignancies and inflammatory conditions.
Biological function summary

CD70 plays an essential role in the regulation of immune responses. It does this by binding to its receptor CD27 forming a receptor-ligand complex that triggers signaling pathways vital for T cell proliferation survival and differentiation. This interaction also influences the production of immunoglobulins by B cells. CD70-CD27 signaling is particularly important in shaping adaptive immunity enhancing the body's ability to respond to pathogens effectively.

Pathways

CD70 is integral in the activation of the NF-κB and MAPK pathways. These pathways are activated upon the interaction of CD70 with CD27 promoting cell survival proliferation and pro-inflammatory cytokine production. Other proteins related to CD70 through these pathways include TRAF2 and TRAF3 which are essential for downstream signaling events that modulate immune responses.

CD70 has been implicated in autoimmune diseases and certain cancers. For instance it plays a role in the pathogenesis of lupus by contributing to abnormal immune activation. Overexpression of CD70 is also associated with various cancers such as renal cell carcinoma where it can support tumor cell growth and immune evasion. Through these conditions CD70 interacts with proteins like BCL-2 in cancer affecting cell survival pathways while its interaction with CD27 influences the immune deregulation seen in lupus.
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Product protocols

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Target data

Expressed at the plasma membrane of B cells, it is the ligand of the CD27 receptor which is specifically expressed at the surface of T cells (PubMed : 28011863, PubMed : 28011864, PubMed : 8387892). The CD70-CD27 signaling pathway mediates antigen-specific T cell activation and expansion which in turn provides immune surveillance of B cells (PubMed : 28011863, PubMed : 28011864).
See full target information CD70
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websiteProtocolBooklet
en
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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