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Human CD74 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human CD74 in Heparin Plasma, Cell culture supernatant, Serum, EDTA Plasma samples.

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Images

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Cell culture supernatant, Serum, EDTA Plasma

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

34.38 - 2200 pg/mL

Assay time

1h 30m

Sensitivity

= 15.63 pg/mL

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Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF.Class-II-associated invariant chain peptideBinds to the peptide-binding site of MHC class II alpha/beta heterodimers forming an alpha-beta-CLIP complex, thereby preventing the loading of antigenic peptides to the MHC class II complex until its release by HLA-DM in the endosome.Isoform p41Stabilizes the conformation of mature CTSL by binding to its active site and serving as a chaperone to help maintain a pool of mature enzyme in endocytic compartments and extracellular space of antigen-presenting cells (APCs). Has antiviral activity by stymieing the endosomal entry of Ebola virus and coronaviruses, including SARS-CoV-2 (PubMed:32855215). Disrupts cathepsin-mediated Ebola virus glycoprotein processing, which prevents viral fusion and entry. This antiviral activity is specific to p41 isoform (PubMed:32855215).

Alternative names

What's included?

1 x 96 Tests
Components
10X Human CD74 Capture Antibody
1 x 600 µL
10X Human CD74 Detector Antibody
1 x 600 µL
10X Wash Buffer PT (ab206977)
1 x 20 mL
Antibody Diluent 5BI
1 x 6 mL
Human CD74 Lyophilized Recombinant Protein
2 x 1 Vial
Plate Seals
1 x 1 Unit
Sample Diluent NS (ab193972)
1 x 12 mL
SimpleStep Pre-Coated 96-Well Microplate (ab206978)
1 x 1 Unit
Stop Solution
1 x 12 mL
TMB Development Solution
1 x 12 mL

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Human CD74 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human CD74 in Heparin Plasma, Cell culture supernatant, Serum, EDTA Plasma samples.

Alternative names

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Cell culture supernatant, Serum, EDTA Plasma

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

34.38 - 2200 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 15.63 pg/mL

Precision

Intra assay

Sample

Serum

n

8

mean

-

SD

-

C.V.

7.5

Inter assay

Sample

Serum

n

3

mean

-

SD

-

C.V.

8.5

Recovery

Sample specific recovery

Sample type

Cell culture supernatant

Average %

= 111

Range

106 - 121 %

Sample type

Serum

Average %

= 94

Range

89 - 103 %

Sample type

EDTA Plasma

Average %

= 90

Range

86 - 95 %

Sample type

Heparin Plasma

Average %

= 95

Range

90 - 102 %

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Human CD74 ELISA kit (ab284618) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human CD74 protein in human serum, plasma, and cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CD74 with 15.63 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Supplementary info

Biological function summary

CD74 facilitates multiple immune regulatory processes. It forms a complex with the MHC II molecules aiding in their proper folding and peptide loading in the endoplasmic reticulum. This complex then travels through the Golgi apparatus towards the lysosomal compartments where antigens are presented to initiate adaptive immune responses. In addition CD74 is involved in signal transduction pathways that regulate cell proliferation and survival influenced by interactions with MIF and other molecules.

Activity summary

CD74 also known as the invariant chain or li is a protein with a mass of about 33 kDa. It is widely expressed on the surface of immune cells such as B cells dendritic cells and macrophages. CD74 plays an important role in the immune system by acting as a chaperone for major histocompatibility complex class II (MHC II) molecules guiding their migration to endosomes where they encounter antigenic peptides. The CD74 protein also functions as a cell surface receptor for macrophage migration inhibitory factor (MIF) enhancing the immune response process.

Pathways

The role of CD74 extends into both the antigen presentation and MIF signaling pathways. Within the antigen presentation pathway CD74's activity is tightly connected with MHC II and subsequently to CD4+ T cells facilitating the activation of these immune cells. The MIF signaling pathway involves CD74 interacting with CD44 a receptor that further propagates the signaling cascades essential for immune modulation and cell survival.

Associated diseases and disorders

CD74 is linked to inflammatory and autoimmune conditions such as rheumatoid arthritis and systemic lupus erythematosus. Aberrant expression of CD74 influences the presentation of autoantigens and may contribute to the chronic inflammation seen in these disorders. Its connection to MIF which is a pro-inflammatory cytokine further implicates CD74 in these autoimmune scenarios by promoting persistent immune activation. Researchers are also exploring CD74 as a therapeutic target in certain cancers due to its influence on immune surveillance and tumor progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Sandwich ELISA - Human CD74 ELISA Kit (ab284618), expandable thumbnail

    Sandwich ELISA - Human CD74 ELISA Kit (ab284618)

    Interpolated concentrations of spiked human CD74 in serum, plasma (heparin), and plasma (EDTA).

    Recombinant CD74 was spiked into the following human samples and diluted in a 2-fold dilution series in Sample Diluent NS. Undiluted samples are 50% serum, plasma (heparin), and plasma (EDTA). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • Sandwich ELISA - Human CD74 ELISA Kit (ab284618), expandable thumbnail

    Sandwich ELISA - Human CD74 ELISA Kit (ab284618)

    Interpolated concentrations of spiked human CD74 in PBMC supernatant stimulated for 46 hours in 1.5% PHA-M.

    Recombinant CD74 was spiked into PBMC supernatant stimulated for 46 hours in 1.5% PHA-M and diluted in a 2-fold dilution series in Sample Diluent NS. Undiluted samples are 50% stimulated PBMC supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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