AB256392

Human CD80 ELISA Kit (B7-1)

Recombinant
SimpleStep
What is this?

Be the first to review this product! Submit a review

(1 Publication)

Human CD80 ELISA Kit (B7-1) is a single-wash 90-min Simplestep used to quantify Human CD80 (B7-1) with a sensitivity of 31 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD80, CD28LG, CD28LG1, LAB7, T-lymphocyte activation antigen CD80, Activation B7-1 antigen, BB1, CTLA-4 counter-receptor B7.1, B7

4 Images

Sandwich ELISA - Human CD80 ELISA Kit (B7-1) (AB256392)

sELISA

Supplier Data

Sandwich ELISA - Human CD80 ELISA Kit (B7-1) (AB256392)

Serum from eight individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD80 concentration was determined to be 780 pg/mL with a range of 572 – 1,371 pg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human CD80 ELISA Kit (B7-1) (AB256392)

Interpolated concentrations of native CD80 in human cell culture supernatant samples.

The concentrations of CD80 were measured in duplicates, interpolated from the CD80 standard curves and corrected for sample dilution. Undiluted samples are as follows : Raji cell supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD80 concentration was determined to be 1,255 pg/mL in Raji cell supernatant 100%.

sELISA

Supplier Data

Sandwich ELISA - Human CD80 ELISA Kit (B7-1) (AB256392)

Interpolated concentrations of native CD80 in human serum and plasmas.

The concentrations of CD80 were measured in duplicates, interpolated from the CD80 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (EDTA) 50%, plasma (citrate) 50%, and plasma heparin 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD80 concentration was determined to be 464 pg/mL in serum, 514 pg/mL in plasma (EDTA), 298 pg/mL in plasma (citrate), and 326 pg/mL in plasma (heparin).

sELISA

Supplier Data

Sandwich ELISA - Human CD80 ELISA Kit (B7-1) (AB256392)

Example of human CD80 standard curve in Sample Diluent NS.

The CD80 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Cow, Human

Assay type

Sandwich (quantitative)

Sensitivity

= 31 pg/mL

Range

78 - 5000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

Product details

Human CD80 ELISA Kit (B7-1) (ab256392) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD80 (B7-1) protein in cell culture media, cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CD80 (B7-1) with 31 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Human CD80, also known as T-lymphocyte activation antigen CD80 and Activation B7-1 antigen, is a B7, type I membrane protein encoded by the gene *CD80*. CD80 is found on the membrane of dendritic cells, activated B and T cells, monocytes, and antigen-presenting cells (APC). CD80 functions as a receptor for CD28 and CTLA-4, which results in T and B-cell activation, proliferation, and differentiation.

style

left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.5" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.9" } ]

style

left-column

Recovery

[ { "sample": "Cell culture supernatant", "range": "106 - 113 %", "average": "= 110" }, { "sample": "Serum", "range": "99 - 105 %", "average": "= 101" }, { "sample": "EDTA Plasma", "range": "94 - 101 %", "average": "= 97" }, { "sample": "Cell culture media", "range": "95 - 100 %", "average": "= 97" }, { "sample": "Heparin Plasma", "range": "88 - 98 %", "average": "= 92" }, { "sample": "Citrate plasma", "range": "99 - 109 %", "average": "= 103" } ]

style

left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab256392-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB256392-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB256392-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB256392-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB256392-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB256392-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CD80 (B7-1) Capture Antibody", "number":"AB256392-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human CD80 (B7-1) Lyophilized Recombinant Protein", "number":"AB256392-CMP08", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB256392-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"10X Human CD80 (B7-1) Detector Antibody", "number":"AB256392-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB256392-CMP05", "productcode":"" } ] } } }

style

left-column

Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD80 also known as B7-1 is a cell surface protein with a molecular weight of approximately 60 kDa. It is expressed on antigen-presenting cells such as dendritic cells macrophages and B cells. The CD80 protein plays a mechanical role in immune response modulation by providing necessary signals for T cell activation and survival. CD80 interacts with receptors CD28 and CTLA-4 on T cells serving as a costimulatory molecule important for productive immune responses.

Biological function summary

CD80 plays a significant role in the immune system's ability to distinguish between self and non-self. It belongs to the B7 family and participates in the formation of immunological synapses between T cells and antigen-presenting cells. This interaction is essential for triggering T cell proliferation and cytokine production forming a part of the adaptive immune response. CD80's expression increases in response to pathogen recognition enhancing the immune system's efficiency.

Pathways

CD80 is integral to the regulation of T cell-mediated immune responses. It is involved in the CD28 co-stimulatory pathway which is vital for T cell activation and function. In this pathway CD80 interacts with proteins like CD28 and CTLA-4 to modulate immune activation and regulate immune tolerance. The proper function of CD80 within this pathway ensures a balanced immune reaction preventing autoimmunity while allowing effective defense against pathogens.

CD80 plays a role in autoimmune conditions and transplantation rejection. Its overexpression or dysregulation can contribute to diseases such as rheumatoid arthritis where it modifies T cell response and inflammation. Additionally CD80 is implicated in organ transplantation influencing graft rejection outcomes due to its role in modulating immune activation. Understanding CD80 interactions with CTLA-4 and CD28 offers insight into these conditions providing potential targets for therapeutic intervention.

style

left-column

Product protocols

style

left-column

Target data

Costimulatory molecule that belongs to the immunoglobulin superfamily that plays an important role in T-lymphocyte activation (PubMed : 38467718). Acts as the primary auxiliary signal augmenting the MHC/TCR signal in naive T-cells together with the CD28 receptor which is constitutively expressed on the cell surface of T-cells (PubMed : 12196291). In turn, activates different signaling pathways such as NF-kappa-B or MAPK leading to the production of different cytokines (PubMed : 10438913). In addition, CD28/CD80 costimulatory signal stimulates glucose metabolism and ATP synthesis of T-cells by activating the PI3K/Akt signaling pathway (PubMed : 12121659). Acts also as a regulator of PDL1/PDCD1 interactions to limit excess engagement of PDL1 and its inhibitory role in immune responses (PubMed : 36727298). Expressed on B-cells, plays a critical role in regulating interactions between B-cells and T-cells in both early and late germinal center responses, which are crucial for the generation of effective humoral immune responses (By similarity).. (Microbial infection) Acts as a receptor for adenovirus subgroup B.

See full target information CD80

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

eLife 10: PubMed34854378

2021

Single-cell-level protein analysis revealing the roles of autoantigen-reactive B lymphocytes in autoimmune disease and the murine model.

Applications

Unspecified application

Species

Unspecified reactive species

Takemichi Fukasawa,Ayumi Yoshizaki,Satoshi Ebata,Asako Yoshizaki-Ogawa,Yoshihide Asano,Atsushi Enomoto,Kiyoshi Miyagawa,Yutaka Kazoe,Kazuma Mawatari,Takehiko Kitamori,Shinichi Sato

View all publications

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human CD80 ELISA Kit (B7-1)

Sandwich ELISA - Human CD80 ELISA Kit (B7-1) (AB256392)

Sandwich ELISA - Human CD80 ELISA Kit (B7-1) (AB256392)

Sandwich ELISA - Human CD80 ELISA Kit (B7-1) (AB256392)

Sandwich ELISA - Human CD80 ELISA Kit (B7-1) (AB256392)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Complementary Products

Secondary Antibodies

Primary Antibodies

Assay Kits

Assay Kits

Product promise