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AB277711

Human CD83 ELISA Kit

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Human CD83 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CD83 with a sensitivity of 2.32 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD83, CD83 antigen, hCD83, B-cell activation protein, Cell surface protein HB15

2 Images
Sandwich ELISA - Human CD83 ELISA Kit (AB277711)
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Supplier Data

Sandwich ELISA - Human CD83 ELISA Kit (AB277711)

Interpolated concentrations of native CD83 in human cell culture supernatant samples.

The concentrations of CD83 were measured in duplicates, interpolated from the CD83 standard curves and corrected for sample dilution. Undiluted samples are as follows : HDLM-2 supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD83 concentration was determined to be 92.03 pg/mL in HDLM-2 supernatant.

Sandwich ELISA - Human CD83 ELISA Kit (AB277711)
  • sELISA

Unknown

Sandwich ELISA - Human CD83 ELISA Kit (AB277711)

Example of human CD83 standard curve in Sample Diluent NS. The CD83 standard curve was prepared as described in Section 10.

Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 2.32 pg/mL

Range

9.38 - 600 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human CD83 ELISA kit (ab277711) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human CD83 protein in human serum, plasma, and cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CD83 with 2.32 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

CD83 is an activation marker for antigen presenting cells and is expressed on activated dendritic cells, interdigitating reticulum cells, and Langerhans cells. A soluble form of CD83 exists in serum that can inhibit maturation of dendritic cells. Elevated levels of CD83 are seen in autoimmune disease and hematological malignancies.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.2" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.7" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "100 - 106 %", "average": "= 104" }, { "sample": "Serum", "range": "98 - 108 %", "average": "= 104" }, { "sample": "EDTA Plasma", "range": "92 - 111 %", "average": "= 101" }, { "sample": "Heparin Plasma", "range": "88 - 111 %", "average": "= 99" }, { "sample": "Citrate plasma", "range": "89 - 106 %", "average": "= 98" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab277711-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB277711-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB277711-CMP11", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB277711-CMP12", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human CD83 Lyophilized Recombinant Protein", "number":"AB277711-CMP08", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB277711-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CD83 Capture Antibody", "number":"AB277711-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB277711-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB277711-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB277711-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CD83 Detector Antibody", "number":"AB277711-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD83 also known as 3D11 in some contexts is a protein with important roles in the immune system. The CD83 protein is a glycoprotein with a molecular mass of approximately 45 kDa. It is expressed in various immune cells including dendritic cells B cells thymic epithelial cells and activated T cells. CD83 acts as a surface marker during the maturation and activation of these cells. This characteristic makes it a useful marker in immunological studies often referred to by scientists as the CD83 marker.
Biological function summary

The primary role of CD83 involves the regulation of immune responses. It functions by modulating the activity and communication of immune cells especially impacting the antigen-presenting capabilities of dendritic cells. CD83 also plays a role in promoting the maturation of these cells and influencing T-cell responses. Although CD83 is not generally recognized as a part of a complex its interactions with other cell surface molecules are significant for immune system function.

Pathways

CD83 has notable involvement in immune signaling pathways including the NF-kB and JAK/STAT pathways. These pathways are critical for transmitting signals that influence cell survival proliferation and activation. Through these pathways CD83 interacts with proteins such as cytokines and growth factors which further modulate their effects on immune cells and their responses.

Understanding CD83’s role provides insights into conditions like autoimmune diseases and immune deficiencies. In autoimmune diseases irregular expression or function of CD83 can disrupt normal immune response contributing to pathogenesis. Its relationship with other proteins such as CD28 in T-cell activation underlies its involvement in disorders characterized by immune dysregulation. Furthermore CD83 expression profiles may help decipher cancer immune evasion mechanisms providing possible therapeutic targets.
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Product protocols

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Target data

May play a significant role in antigen presentation or the cellular interactions that follow lymphocyte activation.
See full target information CD83
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websiteProtocolBooklet
en
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