Human CD85d ELISA Kit (LILRB2) is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human CD85d (LILRB2) in Heparin Plasma, Citrate plasma, Serum, EDTA Plasma samples.
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- World journal of gastroenterology 29:5313-53262023Leukocyte immunoglobulin-like receptor B2 overexpression as a promising therapeutic target and noninvasive screening biomarker for colorectal cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQian-Qian Wang,Lei Zhou,Geng Qin,Chang Tan,Yuan-Chen Zhou,Shu-Kun YaoPubMed 37899785
Key facts
- Detection method
Colorimetric
- Sample types
Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
62.5 - 4000 pg/mL
- Assay time
1h 30m
- Sensitivity
= 15.9 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C, HLA-G and HLA-F alleles (PubMed:11169396, PubMed:12853576, PubMed:16455647, PubMed:20448110, PubMed:27859042). Involved in the down-regulation of the immune response and the development of tolerance. Recognizes HLA-G in complex with B2M/beta-2 microglobulin and a nonamer self-peptide (peptide-bound HLA-G-B2M) triggering differentiation of type 1 regulatory T cells and myeloid-derived suppressor cells, both of which actively maintain maternal-fetal tolerance (PubMed:16455647, PubMed:20448110, PubMed:27859042). Competes with CD8A for binding to class I MHC antigens. Inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions (PubMed:11875462, PubMed:12853576, PubMed:9548455, PubMed:9842885).
Alternative names
CD85d, ILT4, LIR2, MIR10, LILRB2, Leukocyte immunoglobulin-like receptor subfamily B member 2, LIR-2, Leukocyte immunoglobulin-like receptor 2, CD85 antigen-like family member D, Immunoglobulin-like transcript 4, Monocyte/macrophage immunoglobulin-like receptor 10, ILT-4, MIR-10
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Human CD85d ELISA Kit (LILRB2) is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human CD85d (LILRB2) in Heparin Plasma, Citrate plasma, Serum, EDTA Plasma samples.
Key facts
- Detection method
Colorimetric
- Sample types
Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
62.5 - 4000 pg/mL
- Assay time
1h 30m
- Assay Platform
Pre-coated microplate (12 x 8 well strips)
- Sensitivity
= 15.9 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 2.1 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 5.6 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 91 | Range 95 - 100 % |
Sample type EDTA Plasma | Average % = 102 | Range 94 - 107 % |
Sample type Heparin Plasma | Average % = 84 | Range 89 - 93 % |
Sample type Citrate plasma | Average % = 95 | Range 94 - 100 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Human CD85d ELISA Kit (LILRB2) (ab269551) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD85d (LILRB2) protein in edta plasma, hep plasma, serum, and cit plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CD85d (LILRB2) with 15.9 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
CD85d (LILRB2), Leukocyte immunoglobulin-like receptor subfamily B member 2, is a key protein in the regulation of the immune response where it plays a pivotal as a negative regulator. It is primarily found on immune cells where it interacts with MHC domains on antigen presenting cells. Given its important role in immunosuppression, it is currently under investigation as a drug-targetable protein for a host of disease states. The antibodies for this kit were raised against a recombinant protein fragment targeting the extracellular domain of this receptor. There is little homology between mouse and rat for this fragment, so reactivity is not likely.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ILT-4 also known as Immunoglobulin-like transcript 4 is a transmembrane protein with a molecular weight of approximately 45 kDa. The protein is part of the LILR family and is sometimes referred to as LILRB2 protein. ILT-4 is mainly expressed on myeloid lineage cells including monocytes and dendritic cells. The expression of ILT-4 is important for the modulation of the immune response. Biotinylated ILT-4 and biotinylated LILRB2 versions are used frequently in research to study their interactions and functions.
- Biological function summary
ILT-4 plays an important role in the regulation of immune tolerance and inhibition. It is not part of a complex but interacts with other immune regulatory proteins. By binding to MHC class I molecules ILT-4 inhibits the activation of immune responses preventing over-activation and autoimmunity. The ILT-4 protein is critical for helping myeloid cells to maintain a balanced immune response often restricting unnecessary inflammation and promoting tolerance.
- Pathways
ILT-4 influences immune signaling pathways such as those that control T cell receptor signaling. ILT-4 interactions with proteins like CD85d and other LILRA6 proteins showcase its involvement in negative regulatory signaling pathways. These pathways are essential for maintaining immune homeostasis and limiting immune-mediated damage during infections or tissue injury.
- Associated diseases and disorders
ILT-4 is implicated in cancer and autoimmune diseases such as multiple sclerosis. In cancers ILT-4 modulates the immune environment allowing tumors to evade immune surveillance. In the context of autoimmune disorders aberrant ILT-4 expression or function can lead to insufficient immune regulation contributing to disease progression. CD85d and LILRB2 proteins are also associated with these conditions further influencing ILT-4's role in disease mechanisms.
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2 product images
Sandwich ELISA - Human CD85d ELISA Kit (LILRB2) (ab269551)
Example of human CD85d standard curve in Sample Diluent NS.
The CD85d standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human CD85d ELISA Kit (LILRB2) (ab269551)
Interpolated concentrations of native CD85d in human serum and plasma samples.
The concentrations of CD85d were measured in duplicates, interpolated from the CD85d standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 30%, plasma (EDTA) 30%, plasma (citrate) 30%, and plasma (heparin) 30%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD85d concentration was determined to be 8.85 ng/mL in serum, 6.75 ng/mL in plasma (EDTA), 8.82 ng/mL in plasma (citrate), and 7.72 ng/mL in plasma (Heparin).
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