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AB45921

Human CD86 ELISA Kit

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(3 Publications)

Human CD86 ELISA Kit is a Sandwich ELISA for the measurement of Human CD86 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

CD86, CD28LG2, T-lymphocyte activation antigen CD86, Activation B7-2 antigen, B70, BU63, CTLA-4 counter-receptor B7.2, FUN-1

4 Images
ELISA - Human CD86 ELISA Kit (AB45921)
  • ELISA

Lab

ELISA - Human CD86 ELISA Kit (AB45921)

Standard curve with background signal subtracted (duplicates; +/- SD).

ELISA - Human CD86 ELISA Kit (AB45921)
  • ELISA

Lab

ELISA - Human CD86 ELISA Kit (AB45921)

CD86 measured in undiluted 72 hours culture supernatants from control of treated (5 ug x mL-1 PHA; Sigma) PBMCs (1e6 cells x mL-1; duplicates +/- SD).

Sandwich ELISA - Human CD86 ELISA Kit (AB45921)
  • sELISA

Supplier Data

Sandwich ELISA - Human CD86 ELISA Kit (AB45921)

Representative Standard Curve using ab45921

ELISA - Human CD86 ELISA Kit (AB45921)
  • ELISA

Lab

ELISA - Human CD86 ELISA Kit (AB45921)

CD86 in U937 lysates from control cells or cells stimulated for 24 hours with 50 ng x mL-1 PMA (ab120297) or PMA with 1 ug x mL-1 LPS for the last 6 hours (duplicates; +/- SD).

2e6 snap frozen cells were lysed in 0.3 mL RIPA lysis buffer on ice, sonicated in an ice cold water bath for 12 intervals for a total of 3.5 minutes. The supernatants were cleared by centrifugation (max speed, 10 minutes, 4 degrees), and tested undiluted or diluted 1/4.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.6 U/mL

Range

1.187 - 38 U/mL

Assay time

2h 45m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Human CD86 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of CD86 in Human serum, plasma, buffered solutions or cell culture medium.

A monoclonal antibody specific for CD86 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD86 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for CD86 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of CD86 present in the samples.

This kit will recognize both endogenous and recombinant Human CD86.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Sample A", "replicates": 8, "mean": "37.25 U/mL", "standardDeviation": "0.329", "coefficientOfVariability": "= 0.88" }, { "reproducibilityType": "Intra", "sample": "Sample B", "replicates": 8, "mean": "5.09 U/mL", "standardDeviation": "0.193", "coefficientOfVariability": "= 3.79" }, { "reproducibilityType": "Inter", "sample": "Sample B", "replicates": 39, "mean": "4.92 U/mL", "standardDeviation": "0.348", "coefficientOfVariability": "= 7.06" }, { "reproducibilityType": "Inter", "sample": "Sample A", "replicates": 39, "mean": "37.63 U/mL", "standardDeviation": "0.611", "coefficientOfVariability": "= 1.62" } ]
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What's included?

{ "values": { "2x96Tests": { "sellingSize": "2 x 96 Tests", "publicAssetCode":"ab45921-2x96Tests", "assetComponentDetails": [ { "size":"1 x 23 mL", "name":"HRP Diluent", "number":"AB45921-CMP03" }, { "size":"2 x 11 mL", "name":"Stop Reagent", "number":"AB45921-CMP02" }, { "size":"2 x 1 Unit", "name":"CD86 Microplate (12 x 8 well strips)", "number":"AB45921-CMP08" }, { "size":"4 x 5 µL", "name":"Streptavidin-HRP", "number":"AB45921-CMP04" }, { "size":"1 x 25 mL", "name":"10X Standard Diluent Buffer", "number":"AB45921-CMP06" }, { "size":"4 x 1 Vial", "name":"CD86 Standard (Lyophilized)", "number":"AB45921-CMP09" }, { "size":"1 x 13 mL", "name":"Biotinylated Antibody Diluent", "number":"AB45921-CMP01" }, { "size":"2 x 10 mL", "name":"200X Wash Buffer", "number":"AB45921-CMP05" }, { "size":"1 x 24 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB45921-CMP10" }, { "size":"2 x 0.4 mL", "name":"Biotinylated anti-CD86", "number":"AB45921-CMP07" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab45921-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"HRP Diluent", "number":"AB45921-CMP03", "productcode":"" }, { "size":"2 x 5 µL", "name":"Streptavidin-HRP", "number":"AB45921-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"CD86 Standard (Lyophilized)", "number":"AB45921-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"10X Standard Diluent Buffer", "number":"AB45921-CMP06", "productcode":"" }, { "size":"1 x 7 mL", "name":"Biotinylated Antibody Diluent", "number":"AB45921-CMP01", "productcode":"" }, { "size":"1 x 0.4 mL", "name":"Biotinylated anti-CD86", "number":"AB45921-CMP07", "productcode":"" }, { "size":"1 x 10 mL", "name":"200X Wash Buffer", "number":"AB45921-CMP05", "productcode":"" }, { "size":"1 x 11 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB45921-CMP10", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Reagent", "number":"AB45921-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"CD86 Microplate (12 x 8 well strips)", "number":"AB45921-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD86 also known as B7-2 is a protein involved in the regulation of the immune response. It has an approximate mass of 70 kDa and is expressed on antigen-presenting cells like dendritic cells monocytes and macrophages. Notably CD86 is present on macrophages including those in tissues such as skin and lymphoid organs. Expressed on these cells CD86 serves as a vital mediator in the co-stimulatory signals necessary for T cell activation and survival.
Biological function summary

CD86 plays a significant role in the immune system by providing secondary signals for T cell activation and differentiation. It is a part of the B7 protein family and forms a complex with CD28 and CTLA-4 on T cells. When CD86 binds to CD28 it sends positive co-stimulatory signals which promote T cell proliferation and cytokine production. On the other hand interaction with CTLA-4 transmits an inhibitory signal which reduces immune response. This dual interaction helps to balance immune activation and tolerance.

Pathways

CD86 takes part in important immune-related signaling pathways particularly the T cell receptor signaling pathway and the PI3K-Akt signaling pathway. Both pathways are fundamental for initiating immune responses. CD86's interaction with CD28 activates downstream signaling cascades including PI3K-Akt which is important for cell survival and growth. Additionally CD86 collaborates with other proteins such as CD80 another co-stimulatory molecule to amplify T cell activation within these pathways.

CD86 is associated with autoimmune diseases and transplant rejection. In autoimmune diseases like rheumatoid arthritis the overexpression or dysregulation of CD86 can lead to excessive T cell activation causing immune system attacks on the body's own tissues. Similarly in transplant rejection CD86 may contribute by enhancing immune response against transplanted organs. The engagement between CD86 and CD28 is a critical factor in these conditions and therapies targeting this interaction are under exploration to mitigate the immune response.
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Product protocols

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Target data

Costimulatory molecule that belongs to the immunoglobulin superfamily that plays an important role in T-lymphocyte activation (PubMed : 12196291, PubMed : 7694363). Acts as the primary auxiliary signal augmenting the MHC/TCR signal in naive T-cells by acting as a ligand for the CD28 receptor which is constitutively expressed on the cell surface of T-cells (PubMed : 12196291, PubMed : 7694363). May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation (PubMed : 7527824). Also involved in the regulation of B cells function, plays a role in regulating the level of IgG(1) produced. Upon CD40 engagement, activates NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation (By similarity). Also acts as an inhibitor of T-cell activation by acting as a ligand for CTLA4, a decoy receptor, thereby blocking CD28-mediated T-cell priming (PubMed : 11279501).. Isoform 2. Interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.. (Microbial infection) Acts as a receptor for adenovirus subgroup B.
See full target information CD86
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cardiovascular diabetology 22:80 PubMed37005586

2023

SGLT2-inhibitors effects on the coronary fibrous cap thickness and MACEs in diabetic patients with inducible myocardial ischemia and multi vessels non-obstructive coronary artery stenosis.

Applications

Unspecified application

Species

Unspecified reactive species

Celestino Sardu,Maria Consiglia Trotta,Ferdinando Carlo Sasso,Cosimo Sacra,Gerardo Carpinella,Ciro Mauro,Fabio Minicucci,Paolo Calabrò,Michele D' Amico,Fabrizio D' Ascenzo,Ovidio De Filippo,Mario Iannaccone,Carmine Pizzi,Giuseppe Paolisso,Raffaele Marfella

Multiple sclerosis and related disorders 25:37-42 PubMed30031282

2018

Increased CCL18 plasma levels are associated with neurodegenerative MRI outcomes in multiple sclerosis patients.

Applications

Unspecified application

Species

Unspecified reactive species

Nicole Ziliotto,Francesco Bernardi,Dejan Jakimovski,Marcello Baroni,Niels Bergsland,Deepa P Ramasamy,Bianca Weinstock-Guttman,Paolo Zamboni,Giovanna Marchetti,Robert Zivadinov,Murali Ramanathan

Brain : a journal of neurology 141:786-796 PubMed29361022

2018

Multiple sclerosis risk variants alter expression of co-stimulatory genes in B cells.

Applications

sELISA

Species

Unspecified reactive species

Ide Smets,Barnaby Fiddes,Josselyn E Garcia-Perez,Di He,Klara Mallants,Wenjia Liao,James Dooley,George Wang,Stephanie Humblet-Baron,Bénédicte Dubois,Alastair Compston,Joanne Jones,Alasdair Coles,Adrian Liston,Maria Ban,An Goris,Stephen Sawcer
View all publications
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