Human CD8A ELISA Kit
- Recombinant
- SimpleStep
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Human CD8A ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CD8A with a sensitivity of 4.7 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
View Alternative Names
CD8a, MAL, CD8A, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2
- sELISA
Supplier Data
Sandwich ELISA - Human CD8A ELISA Kit (AB256393)
Interpolated concentrations of native CD8A in human lymph node and thymus based on a 25 μg/mL and 6.25 µg/mL extract load respectively.
The concentrations of CD8A were measured in duplicate and interpolated from the CD8A standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
- sELISA
Supplier Data
Sandwich ELISA - Human CD8A ELISA Kit (AB256393)
Example of human CD8A standard curve in Sample Diluent NS.
The CD8A standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Reactivity data
Product details
Human CD8A ELISA Kit (ab256393) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD8A protein in cell culture supernatant, edta plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CD8A with 4.7 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Human CD8 alpha, or T-Cell surface glycoprotein CD8 alpha chain, is a cell surface glycoprotein encoded by the gene Cd8a. CD8A is found on cytotoxic T lymphocytes and helps mediates cell-cell interactions within the immune system. CD8 acts a coreceptor for MHC class 1/peptide complex either as a homodimer of two alpha chains or as a heterodimer of one alpha chain and one beta chain. CD8A interacts with LCK in a zinc-dependent manner, which initiates intracellular signaling pathways resulting in activation of cytotoxic T-lymphocytes.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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Recovery
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Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The CD8 alpha protein plays a critical role in T-cell mediated immune responses. It forms a heterodimer with the CD8 beta chain creating the CD8 alpha-beta complex that strengthens T-cell interaction with antigen-presenting cells. CD8 alpha also helps in signaling processes that activate T cells equipping them to destroy infected or malignant cells. Researchers often study CD8 alpha peptides to understand its interactions better.
Pathways
CD8 alpha is integral to the T-cell receptor signaling pathway and the cytotoxic T lymphocyte (CTL) pathway. The T-cell receptor complex which includes the CD8 molecule transmits signals that are important for T-cell activation and function. CD8 interacts with key proteins such as the T-cell receptor (TCR) and MHC class I molecules facilitating targeted responses against pathogens. These pathways highlight CD8 alpha’s role in adaptive immunity.
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