Human CD93 ELISA Kit (C1qR)

Product details

The Human CD93 Enzyme-Linked Immunosorbent Assay (ELISA) kit (C1qR) (ab213762) is designed for the quantitative measurement of Human CD93 in cell culture supernatants, serum, plasma (heparin, EDTA), saliva and urine.

The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for CD93/C1qR has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: A24-K580) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD93/C1qR is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human CD93/C1qR amount of sample captured in plate.

CD93 (Cluster of Differentiation 93) is a protein that in humans is encoded by the CD93 gene. CD93 belongs to the Group XIV C-Type lectin family and is mapped to 20p11.21. The protein encoded by this gene is a cell-surface glycoprotein and type I membrane protein that was originally identified as a myeloid cell-specific marker. The encoded protein was once thought to be a receptor for C1q, but now is thought to instead be involved in intercellular adhesion and in the clearance of apoptotic cells. The intracellular cytoplasmic tail of this protein has been found to interact with moesin, a protein known to play a role in linking transmembrane proteins to the cytoskeleton and in the re-modeling of the cytoskeleton. It has also showed that this receptor is a positive marker for all bone marrow-repopulating stem cells because it is expressed on both CD34-negative and CD34-positive stem cells from umbilical cord blood and adult bone marrow. In addition, highly purified lineage-negative cells with the CD93 protein not only have bone marrow-repopulating capacity but also can differentiate into human hepatocytes in vivo.