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AB253216

Human CDCP1 ELISA Kit

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(1 Publication)

Human CDCP1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CDCP1 with a sensitivity of 0.221 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD318, TRASK, UNQ2486/PRO5773, CDCP1, CUB domain-containing protein 1, Membrane glycoprotein gp140, Subtractive immunization M plus HEp3-associated 135 kDa protein, Transmembrane and associated with src kinases, SIMA135

2 Images
Sandwich ELISA - Human CDCP1 ELISA Kit (AB253216)
  • sELISA

Unknown

Sandwich ELISA - Human CDCP1 ELISA Kit (AB253216)

Interpolated concentrations of native CDCP1 in HT-29 cell extracts based on a 12.5 μg/mL extract load.

The concentrations of CDCP1 were measured in duplicate and interpolated from the CDCP1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CDCP1 concentration was determined to be 8.36 ng/mL .

Sandwich ELISA - Human CDCP1 ELISA Kit (AB253216)
  • sELISA

Unknown

Sandwich ELISA - Human CDCP1 ELISA Kit (AB253216)

Example of human CDCP1 standard curve in 1X Cell Extraction Buffer PTR.

The CDCP1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.221 ng/mL

Range

0.31 - 20 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

Product details

Human CDCP1 ELISA Kit (ab253216) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CDCP1 protein in tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CDCP1 with 0.221 ng/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

CDCP1 is a transmembrane protein involved in cell adhesion and signaling. Alongside the integrin receptors, CDCP1 helps control focal adhesion proteins, promoting anchorage or detachment of cells. Given its importance in cell motility, CDCP1 has been studied in the context of tumorigenesis where it has been found to be upregulated in many cancer types including breast cancer. It is currently thought to be a main driver of tumor invasion and metastasis.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.2" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 24, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.2" } ]

Recovery

[ { "sample": "Tissue Extracts", "range": "100 - 109 %", "average": "= 106" } ]

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab253216-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB253216-CMP04", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB253216-CMP13", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB253216-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB253216-CMP12", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human CDCP1 Lyophilized Recombinant Protein", "number":"AB253216-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CDCP1 Capture Antibody", "number":"AB253216-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CDCP1 Detector Antibody", "number":"AB253216-CMP08", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB253216-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB253216-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB253216-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB253216-CMP03", "productcode":"" } ] } } }

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C

Product protocols

Target data

May be involved in cell adhesion and cell matrix association. May play a role in the regulation of anchorage versus migration or proliferation versus differentiation via its phosphorylation. May be a novel marker for leukemia diagnosis and for immature hematopoietic stem cell subsets. Belongs to the tetraspanin web involved in tumor progression and metastasis.
See full target information CDCP1

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 14:1332777 PubMed38235129

2024

Early inflammatory markers as prognostic indicators following allogeneic stem cell transplantation.

Applications

Unspecified application

Species

Unspecified reactive species

Kriti Verma,Wayne Croft,David Greenwood,Christine Stephens,Ram Malladi,Jane Nunnick,Jianmin Zuo,Francesca A M Kinsella,Paul Moss
View all publications
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Product promise

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