AB260071

Human CDNF ELISA Kit

Recombinant
SimpleStep
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(1 Publication)

Human CDNF ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CDNF with a sensitivity of 3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

ARMETL1, CDNF, Cerebral dopamine neurotrophic factor, ARMET-like protein 1, Conserved dopamine neurotrophic factor

2 Images

Sandwich ELISA - Human CDNF ELISA Kit (AB260071)

sELISA

Supplier Data

Sandwich ELISA - Human CDNF ELISA Kit (AB260071)

Interpolated concentrations of native CDNF in human brain tissue extract and cerebellum tissue extract based on a 400 μg/mL and 600 μg/mL extract load, respectively.

The concentrations of CDNF were measured in duplicate and interpolated from the CDNF standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CDNF concentration was determined to be 259.16 mg/mL in human brain tissue extract and 203.93 mg/mL in human cerebellum tissue extract.

sELISA

Supplier Data

Sandwich ELISA - Human CDNF ELISA Kit (AB260071)

Example of human CDNF standard curve in 1X Cell Extraction Buffer PTR.

The CDNF standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 3 pg/mL

Range

7.81 - 500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human CDNF ELISA Kit (ab260071) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CDNF protein in tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CDNF with 3 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

CDNF, Cerebral dopamine neurotrophic factor, is a non-classical neurotrophic factor found in the brain and released during ER stress and calcium depletion. It is currently being studied as a potential therapeutic target for Parkinson's disease. This kit targets the mature, full-length, protein form which lacks the N-terminal signaling peptide.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.6" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.4" } ]

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Recovery

[ { "sample": "Tissue Extracts", "range": "97 - 118 %", "average": "= 106" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab260071-1x96Tests", "assetComponentDetails": [ { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB260071-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB260071-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB260071-CMP12", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB260071-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB260071-CMP10", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB260071-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human CDNF Lyophilized Recombinant Protein", "number":"AB260071-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CDNF Capture Antibody", "number":"AB260071-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CDNF Detector Antibody", "number":"AB260071-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB260071-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB260071-CMP05", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CDNF also known as Cerebral Dopamine Neurotrophic Factor is a protein that helps in maintaining the proper function of neurons. It has a mass of approximately 18 kDa. This protein is widely expressed in various tissues especially in the brain. CDNF maintains endoplasmic reticulum homeostasis and is involved in the stabilization of dopaminergic neurons. It does this by reducing stress in the endoplasmic reticulum and promoting cell survival mechanisms.

Biological function summary

The protein ensures the survival of neurons by modulating cellular stress pathways. CDNF is not known to be part of a larger protein complex. Instead its activity relies on its own structural configuration to influence stress responses and neuron protection. As a secreted protein it directly interacts with and influences cellular mechanisms within the extracellular space contributing to a healthy neuronal environment.

Pathways

CDNF interacts with at least oxidative stress response pathways and neuroprotection pathways. These pathways underpin its role in counteracting cellular stress related to neurodegeneration. For example CDNF shares a functional connection with proteins like MANF (Mesencephalic Astrocyte-derived Neurotrophic Factor) another neurotrophic factor involved in similar neuroprotective pathways enhancing neuronal survival and recovery from cellular stress.

Neurodegenerative conditions such as Parkinson's disease and Alzheimer's disease are major areas where CDNF shows relevance. In Parkinson's disease CDNF's neuroprotective role helps mitigate the loss of dopaminergic neurons. There is evidence linking CDNF with MANF which also exhibits protective roles in neuronal health. Both proteins aim to promote neuron survival amid progression of neurodegeneration associated with these diseases indicating their potential as therapeutic agents.

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Product protocols

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Target data

Trophic factor for dopamine neurons. Prevents the 6-hydroxydopamine (6-OHDA)-induced degeneration of dopaminergic neurons. When administered after 6-OHDA-lesioning, restores the dopaminergic function and prevents the degeneration of dopaminergic neurons in substantia nigra (By similarity).

See full target information CDNF

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 14:128 PubMed36792604

2023

Augmenting hematoma-scavenging capacity of innate immune cells by CDNF reduces brain injury and promotes functional recovery after intracerebral hemorrhage.

Applications

Unspecified application

Species

Unspecified reactive species

Kuan-Yin Tseng,Vassilis Stratoulias,Wei-Fen Hu,Jui-Sheng Wu,Vicki Wang,Yuan-Hao Chen,Anna Seelbach,Henri J Huttunen,Natalia Kulesskaya,Cheng-Yoong Pang,Jian-Liang Chou,Maria Lindahl,Mart Saarma,Li-Chuan Huang,Mikko Airavaara,Hock-Kean Liew

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human CDNF ELISA Kit

Sandwich ELISA - Human CDNF ELISA Kit (AB260071)

Sandwich ELISA - Human CDNF ELISA Kit (AB260071)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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Product promise