Human CEA ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CEA with a sensitivity of 24.68 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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- Analytical and bioanalytical chemistry 413:1073-10802020Boronate ester bond-based potentiometric aptasensor for screening carcinoembryonic antigen-glycoprotein using nanometer-sized CaCO with ion-selective electrode.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYe Chen,Bin Li,Peng Lyu,Hang Fai Kwok,Lilin Ge,Qinan WuPubMed 33230701
- Analytica chimica acta 1136:91-982020Thionine-doped nanometer-sized silica conjugated with phenylboronic acid: An innovative recognition/signal element for voltammetric aptasensing of colorectal cancer-related carcinoembryonic antigen.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiangjie Chi et. al.PubMed 33081954
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture extracts, Tissue Extracts, Cell culture media, Heparin Plasma, Citrate plasma, Serum, Cell Lysate, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 78.13 - 5000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 24.68 pg/mL
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Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Cell surface glycoprotein that plays a role in cell adhesion, intracellular signaling and tumor progression (PubMed:10864933, PubMed:10910050, PubMed:2803308). Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6 (PubMed:2803308). Plays a role as an oncogene by promoting tumor progression; induces resistance to anoikis of colorectal carcinoma cells (PubMed:10910050). (Microbial infection) Receptor for E.coli Dr adhesins. Binding of E.coli Dr adhesins leads to dissociation of the homodimer.
Alternative names
CD66e, CEA, CEACAM5, Cell adhesion molecule CEACAM5, Carcinoembryonic antigen, Carcinoembryonic antigen-related cell adhesion molecule 5, Meconium antigen 100, CEA cell adhesion molecule 5
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Recommended products
Human CEA ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CEA with a sensitivity of 24.68 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture extracts, Tissue Extracts, Cell culture media, Heparin Plasma, Citrate plasma, Serum, Cell Lysate, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 78.13 - 5000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 24.68 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Extract | n 8 | C.V. 5 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Extract | n 3 | C.V. 5.4 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 100 | Range 92 - 116 % |
Sample type EDTA Plasma | Average % = 98 | Range 91 - 110 % |
Sample type Cell culture extracts | Average % = 108 | Range 95 - 122 % |
Sample type Cell culture media | Average % = 93 | Range 84 - 99 % |
Sample type Tissue Extracts | Average % = 109 | Range 104 - 117 % |
Sample type Heparin Plasma | Average % = 101 | Range 90 - 110 % |
Sample type Citrate plasma | Average % = 105 | Range 91 - 111 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human CEA ELISA Kit ab264604 is a rapid single-wash 90-min Sandwich ELISA to measure Human CEA in cell culture extracts, cell culture media, cell lysate, citrate plasma, EDTA plasma, heparin plasma, serum, tissue extracts. This SimpleStep sensitivity is 24.68 pg/mL.
How the assay works
Human CEA SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human CEA ELISA Kit ab264604 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-CEACAM5 antibody [EPR22676-37] - BSA and Azide free (Capture) Anti-CEACAM5 antibody [EPR22676-256] - BSA and Azide free (Detector) ab270312
- Anti-CEACAM5 antibody [EPR22676-256] - BSA and Azide free (Detector) Anti-CEACAM5 antibody [EPR22676-256] - BSA and Azide free (Detector) ab270312
CEA is encoded by the CECAM5 gene which produces a cell adhesion molecule consisting of an Ig variable region-like amino-terminal domain followed by up to six Ig constant region-like domains anchored to the cell membrane by either a glycosylphosphatidylinositol (GPI) moiety or a proteinaceous transmembrane and cytoplasmic domain. CEA is normally expressed on epithelial cells and is found abundantly on the apical surface of the gastrointestinal epithelium, as well as the nasopharynx, lung, urogenital tract, and sweat glands. Under physiologic conditions, only low amounts of soluble CEA can be detected in serum. However, in many different cancers, CEA is highly up regulated.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CEACAM5 also known as Carcinoembryonic Antigen (CEA) or CD66e is a glycoprotein found mainly on the surface of epithelial cells. This protein has a mass of approximately 180 kDa. It gets expressed in a variety of tissues with high expression in the gastrointestinal tract including the colon and rectum. CEACAM5 is a member of the immunoglobulin superfamily and plays a role in cell adhesion influencing how cells stick to each other.
- Biological function summary
CEACAM5 contributes to cellular processes involving cell adhesion and immune response modulation. This protein operates as part of a larger group of cell adhesion molecules and does not function as part of a complex. CEACAM5 can affect the interactions between cells and the extracellular matrix which has implications for tissue structuring and integrity. It helps the immune system recognize and respond to infectious agents or cellular anomalies.
- Pathways
CEACAM5 involvement extends to immune response regulation and cell adhesion pathways. It interacts functionally with proteins like E-cadherin in these pathways which contribute to maintaining epithelial integrity. These pathways are critical for normal tissue maintenance and immune system function especially in mucosal surfaces where pathogen entry occurs. Understanding CEACAM5 interactions provides insight into cellular communication and adhesion mechanisms.
- Associated diseases and disorders
CEACAM5 is associated with colorectal cancer and lung adenocarcinoma. Its overexpression and release into the bloodstream make it a popular biomarker for these diseases. CEACAM5 is used in clinical applications such as CEA ELISA assays for cancer detection and monitoring. In colorectal cancer CEACAM5 interacts with proteins like CEACAM6 to promote tumor invasiveness and metastasis. Anti-CEACAM5 therapeutics including antibody-drug conjugates (anti-CEACAM5 ADC) are being developed to target and treat associated malignancies.
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5 product images
Sandwich ELISA - Human CEA ELISA Kit (ab264604)
Human CEA standard curve comparison.
Standard Curve comparison between human CEA SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
Sandwich ELISA - Human CEA ELISA Kit (ab264604)
Human CEA Simplestep ELISA comparison.
Standard Curve comparison between ab264604 and ab183365 SimpleStep ELISA kits. ab264604 SimpleStep ELISA kit shows increased sensitivity.
Sandwich ELISA - Human CEA ELISA Kit (ab264604)
Example of human CEA standard curve in Sample Diluent NS.
The CEA standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human CEA ELISA Kit (ab264604)
Interpolated concentrations of native CEA in human MCF7 and colon homogenate extract samples based on 250 and 125 μg/mL extract loads, respectively.
The concentrations of CEA were measured in duplicate and interpolated from the CEA standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CEA concentration was determined to be 3,584.91 pg/ml in MCF7 and 3,812.56 pg/mL in colon homogenate extract.
Sandwich ELISA - Human CEA ELISA Kit (ab264604)
Serum from ten individual healthy human female donors was measured in duplicate, one donor of which was non-detectible (ND) by this assay.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CEA concentration was determined to be 1,027.57 pg/mL with a range of ND – 4,257.59 pg/mL.
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