AB283545

Human CEACAM6 ELISA Kit

Recombinant
SimpleStep
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Human CEACAM6 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CEACAM6 with a sensitivity of 3.41 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD66c, NCA, CEACAM6, Cell adhesion molecule CEACAM6, Carcinoembryonic antigen-related cell adhesion molecule 6, Non-specific crossreacting antigen, Normal cross-reacting antigen, CEA cell adhesion molecule 6

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Sandwich ELISA - Human CEACAM6 ELISA Kit (AB283545)

sELISA

Supplier Data

Sandwich ELISA - Human CEACAM6 ELISA Kit (AB283545)

Interpolated concentrations of native CEACAM6 in human serum and plasma samples.

The concentrations of CEACAM6 were measured in duplicates, interpolated from the CEACAM6 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 40, plasma (citrate) 1 : 40, plasma (heparin) 1 : 40, and plasma (EDTA) 1 : 40. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CEACAM6 concentration was determined to be 23.01 ng/mL in serum, 19.23 ng/mL in plasma (citrate), 20.26 ng/mL in plasma (heparin), and 22.39 ng/mL in plasma (EDTA).

sELISA

Supplier Data

Sandwich ELISA - Human CEACAM6 ELISA Kit (AB283545)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CEACAM6 concentration was determined to be 28.02 ng/mL with a range of 14.38 – 54.91 ng/mL.

sELISA

Supplier Data

Sandwich ELISA - Human CEACAM6 ELISA Kit (AB283545)

Interpolated concentrations of native CEACAM6 in human PBMC cell culture supernatant samples.

The concentrations of CEACAM6 were measured in duplicates, interpolated from the CEACAM6 standard curves and corrected for sample dilution. Undiluted samples are as follows : PBMC supernatant (50%). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CEACAM6 concentration was determined to be 265.23 pg/mL in PBMC supernatant.

sELISA

Supplier Data

Sandwich ELISA - Human CEACAM6 ELISA Kit (AB283545)

Example of human CEACAM6 standard curve in Sample Diluent NS.

The CEACAM6 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 3.41 pg/mL

Range

10.16 - 650 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human CEACAM6 ELISA kit (ab283545) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human CEACAM6 protein in human serum, plasma and cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CEACAM6 with 3.41 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

CEACAM6 is a cell surface glycoprotein receptor and part of the CEA immunoglobulin superfamily. CEACAM6 is expressed in neutrophils, columnar epithelial and goblet cells, and in many tumor cell lines. It is involved in cell adhesion and tumor progression. Overexpression of CEACAM6 is seen in primary and metastatic pancreatic ductal adenocarcinoma and is a poor prognostic marker.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.8" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "0.1" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "82 - 114 %", "average": "= 96" }, { "sample": "Serum", "range": "98 - 104 %", "average": "= 101" }, { "sample": "EDTA Plasma", "range": "87 - 98 %", "average": "= 91" }, { "sample": "Heparin Plasma", "range": "89 - 100 %", "average": "= 94" }, { "sample": "Citrate plasma", "range": "89 - 100 %", "average": "= 96" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CEACAM6 also known as 9a6 CEACAM-6 and 1h7 is a cell surface glycoprotein weighing approximately 35-40 kDa. The protein plays a mechanical role as an intercellular adhesion molecule. It displays broad expression across multiple tissues with high levels found in epithelial cells of the gastrointestinal tract respiratory tract and in leukocytes.

Biological function summary

CEACAM6 acts in cell signaling and adhesion processes. It often forms complexes with other members of the CEACAM family such as CEACAM5. This protein is involved in the regulation of cell-cell adhesion which impacts cell migration and organization. It also influences immune reactions by modulating the interactions between pathogens and host cells.

Pathways

CEACAM6 integrates into pathways associated with cell adhesion and immune regulation. It engages in the PI3K-Akt signaling pathway tightly interacting with proteins such as CEACAM1 and CEACAM5. Through these interactions CEACAM6 plays an essential role in cell survival proliferation and immune responses.

CEACAM6 shows significant links with cancer especially colorectal and pancreatic cancers. Overexpression of this protein correlates with tumor progression and metastasis. Additionally in inflammatory bowel disease CEACAM6 association with CEACAM7 often results in altered epithelial barrier function contributing to disease pathogenesis.

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Product protocols

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Target data

Cell surface glycoprotein that plays a role in cell adhesion and tumor progression (PubMed : 10910050, PubMed : 11590190, PubMed : 1378450, PubMed : 16204051, PubMed : 2022629, PubMed : 2803308, PubMed : 8776764). Intercellular adhesion occurs in a calcium- and fibronectin-independent manner (PubMed : 16204051, PubMed : 2022629). Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM5 and CEACAM8 (PubMed : 11590190, PubMed : 16204051, PubMed : 2022629, PubMed : 2803308, PubMed : 8776764). Heterophilic interaction with CEACAM8 occurs in activated neutrophils (PubMed : 8776764). Plays a role in neutrophil adhesion to cytokine-activated endothelial cells (PubMed : 1378450). Plays a role in cell migration and cell adhesion to endothelial cells (PubMed : 16204051).

See full target information CEACAM6

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human CEACAM6 ELISA Kit

Sandwich ELISA - Human CEACAM6 ELISA Kit (AB283545)

Sandwich ELISA - Human CEACAM6 ELISA Kit (AB283545)

Sandwich ELISA - Human CEACAM6 ELISA Kit (AB283545)

Sandwich ELISA - Human CEACAM6 ELISA Kit (AB283545)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Assay Kits

Primary Antibodies

Secondary Antibodies

Assay Kits

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise