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AB283982

Human CENP-B ELISA Kit

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Human CENP-B ELISA Kit is a Sandwich ELISA for the measurement of Human CENP-B in Human in Cell/Tissue Extracts samples.

View Alternative Names

Major centromere autoantigen B, Centromere protein B, CENP-B, CENPB

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Sandwich ELISA - Human CENP-B ELISA Kit (AB283982)
  • sELISA

Supplier Data

Sandwich ELISA - Human CENP-B ELISA Kit (AB283982)

Example of Human CENPB standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.42 ng/mL

Range

0.625 - 40 ng/mL

Assay time

4h

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB25734

Anti-CENPB antibody

KO Validated

Western blot - Anti-CENPB antibody (AB25734)

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Primary Antibodies

AB45694

Anti-CENPA antibody [EP800Y] - Centromere Marker

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CENPA antibody [EP800Y] - Centromere Marker (AB45694)

  • 1
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Proteins & Peptides

AB275796

Recombinant Human CENPB protein (His tag)

SDS-PAGE - Recombinant Human CENPB protein (His tag) (AB275796)

  • 0
  • 0
Primary Antibodies

AB259849

Anti-Cbx8 antibody [EPR23800-14]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-Cbx8 antibody [EPR23800-14] (AB259849)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

The Human CENP-B ELISA Kit (ab283982) is designed for detection of CENP-B in human cell lysate samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures human CENP-B in approximately 4 hours.

A polyclonal antibody specific for human CENP-B has been pre-coated onto a 96-well microplate with removable strips. CENP-B in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human CENP-B, which is recognized by a streptavidin-peroxidase (SP) conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The colour development is stopped and the intensity of the colour is measured.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 60, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.1" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 60, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CENP-B also known as Centromere Protein B is a protein with a molecular weight of approximately 80 kDa. It binds to the centromeric DNA and plays a critical role in centromere formation and function. CENP-B is heavily expressed in dividing cells particularly in tissues where cell proliferation is high such as bone marrow and epithelial tissues. Researchers often use tools like anti-CENP-B antibodies and CENP-B ELISA kits to study its expression in various experimental settings.
Biological function summary

CENP-B participates in the assembly of the centromere protein complex contributing to the stability and maintenance of the centromere structure during cell division. It acts as a foundation for kinetochore nucleation ensuring proper chromosome segregation. CENP-B is involved in the formation of the CENP-A nucleosome complex important for accurate chromosomal alignment and separation.

Pathways

CENP-B is essential in the mitotic spindle assembly and chromosome segregation pathways. This protein interacts with CENP-A a variant histone which integrates into centromeric nucleosomes to initiate the formation of functional kinetochores. Additionally CENP-B interacts with CENP-C facilitating the recruitment of other centromere-associated proteins needed for chromosome dynamics during mitosis.

CENP-B is primarily associated with autoimmune disorders such as systemic sclerosis (scleroderma). Patients with these conditions often produce anti-CENP-B antibodies which serve as diagnostic markers. Moreover CENP-B's dysregulation links to chromosomal instability a contributor to cancer. Research has shown connections between CENP-B and related proteins like CENP-A in the context of chromosomal aneuploidies and malignancies.
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Product protocols

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Target data

Interacts with centromeric heterochromatin in chromosomes and binds to a specific 17 bp subset of alphoid satellite DNA, called the CENP-B box (PubMed : 11726497). May organize arrays of centromere satellite DNA into a higher-order structure which then directs centromere formation and kinetochore assembly in mammalian chromosomes (Probable).
See full target information CENPB
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websiteProtocolBooklet
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Product promise

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