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AB313529

Human CES2 ELISA Kit

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Human CES2 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human CES2 in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

ICE, CES2, Cocaine esterase, Carboxylesterase 2, Methylumbelliferyl-acetate deacetylase 2, CE-2, hCE-2

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Sandwich ELISA - Human CES2 ELISA Kit (AB313529)
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Supplier Data

Sandwich ELISA - Human CES2 ELISA Kit (AB313529)

Example of standard curve using ab313529. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 1.6 ng/mL

Range

1.6 - 400 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human CES2 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CES2 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human CES2 coated on a 96-well plate.

This ELISA kit shows no cross-reactivity with the following cytokines tested: human AMIGO2, Arginase 1, B7-H4, Bcl-10, CD42b, CD73, CES1, cIAP-1, Cyclophilin A, Cystatin S, DNMT3A, Epimorphin, GDF-9, Glypican 3, GPR115, HE4, HO-1, HS3ST4, IGSF3, IL-17 RD, Integrin alpha 1, KIR2DL3, LAMP2, LEDGF, MOG, Nestin, Neudesin, Neuroligin 2, NKp80, Osteoadherin, PDGF R alpha, PRDX4, Syntaxin 4, TAFA1, TAFA2, TAFA5, Tenascin R, TGM4, TMEFF1.

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Recovery

[ { "sample": "Serum", "range": "71 - 146 %", "average": "= 106.3" }, { "sample": "Plasma", "range": "70 - 135 %", "average": "= 87.1" }, { "sample": "Cell culture media", "range": "71 - 143 %", "average": "= 108.6" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab313529-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB313529-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB313529-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human CES2 Protein Standard", "number":"AB313529-CMP10", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB313529-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human CES2 Detection Antibody", "number":"AB313529-CMP09", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB313529-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human CES2 Antibody-coated ELISA Plate", "number":"AB313529-CMP08", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin", "number":"AB313529-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Carboxylesterase 2 (CES2) also known as intestinal carboxylesterase is an enzyme with a molecular mass of approximately 60 kDa. CES2 hydrolyzes esters and amides affecting metabolism of drugs and xenobiotics. It is found mainly in the liver and small intestine with lesser expression in the colon kidney and brain. This distribution enables CES2 to play a role in both intestinal and systemic drug metabolism.
Biological function summary

CES2 acts in the hydrolysis process converting prodrugs into active compounds. It functions independently and is not typically part of a larger complex. This enzyme demonstrates broad substrate specificity impacting the metabolic activation of several therapeutic agents. Its activity affects the bioavailability and activation of medicines such as irinotecan and capecitabine which are used in cancer treatments.

Pathways

CES2 takes part in the drug metabolism pathway involving cytochrome P450 enzymes. It interacts with CYP3A4 one of the key enzymes in this system and facilitates the conversion of chemical compounds into pharmacologically active or inactive metabolites. CES2 also participates in lipid metabolism affecting the ester and triglyceride hydrolysis alongside lipases and other related enzymes.

CES2 has implications in colorectal cancer and liver disease conditions where altered drug metabolism influences disease progression and treatment efficacy. It shows associations with irinotecan activation a drug used for treating colorectal cancer and links with UDP-glucuronosyltransferases (UGTs) that also engage in drug metabolism. Understanding CES2's function aids in predicting patient responses to drug therapies and refining treatment strategies for these conditions.
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Product protocols

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Target data

Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs (PubMed : 9169443). Shows high catalytic efficiency for hydrolysis of cocaine, 4-methylumbelliferyl acetate, heroin and 6-monoacetylmorphine (PubMed : 9169443). Hydrolyzes aspirin, substrates with large alcohol group and small acyl group and endogenous lipids such as triacylglycerol (PubMed : 28677105). Converts monoacylglycerides to free fatty acids and glycerol. Hydrolyzes of 2-arachidonoylglycerol and prostaglandins (PubMed : 21049984).
See full target information CES2
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websiteProtocolBooklet
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AB250793

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