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AB314833

Human CFHR1 ELISA Kit

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Human CFHR1 ELISA Kit is a Sandwich ELISA for the measurement of Human CFHR1 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

CFHL, CFHL1, CFHL1P, CFHR1P, FHR1, HFL1, HFL2, CFHR1, Complement factor H-related protein 1, FHR-1, H factor-like protein 1, H36, FHL-1, H-factor-like 1

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Sandwich ELISA - Human CFHR1 ELISA Kit (AB314833)
  • sELISA

Supplier Data

Sandwich ELISA - Human CFHR1 ELISA Kit (AB314833)

Example of standard curve using ab314833. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.14 ng/mL

Range

0.14 - 100 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB305137

Anti-CFHR1 antibody [EPR25757-16] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-CFHR1 antibody [EPR25757-16] - BSA and Azide free (Detector) (AB305137)

  • 0
  • 0
Proteins & Peptides

AB152006

Recombinant Human CFHR1 protein

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human CFHR1 ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CFHR1 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human CFHR1 coated on a 96well plate.
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Recovery

[ { "sample": "Serum", "range": "96 - 125 %", "average": "= 109.4" }, { "sample": "Plasma", "range": "98 - 118 %", "average": "= 109.5" }, { "sample": "Cell culture media", "range": "91 - 102 %", "average": "= 97.66" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab314833-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Human CFHR1 Antibody-coated ELISA Plate", "number":"AB314833-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB314833-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB314833-CMP07", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB314833-CMP04", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB314833-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"400X HRP-Streptavidin", "number":"AB314833-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human CFHR1 Protein Standard", "number":"AB314833-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human CFHR1 Detection Antibody", "number":"AB314833-CMP09", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CFHR1 also known as Complement Factor H-Related protein 1 plays a role in the regulation of the complement system. This protein has a molecular mass of approximately 37 kDa. CFHR1 is mainly expressed in the liver and is present in plasma. The protein has structural similarities to Factor H with homologous domains known as short consensus repeats which contribute to its function in modulating complement activity.
Biological function summary

CFHR1 regulates the alternative pathway of the complement system. It can bind to complement component C3b inhibiting its activity and preventing over-activation of the complement cascade. CFHR1 does not function alone; it interacts with other proteins in the complement system although it forms no stable complex. Through these interactions CFHR1 maintains homeostasis in immune responses.

Pathways

CFHR1 participates significantly in the alternative complement pathway. It works alongside other complement factors such as Factor H to ensure balanced complement activation and regulation. CFHR1 indirectly influences the inflammatory response by regulating this pathway linking innate immunity processes with complement-mediated functions.

CFHR1 is linked to atypical hemolytic uremic syndrome (aHUS) and age-related macular degeneration (AMD). In aHUS CFHR1 gene deletions can lead to unrestrained complement activation due to lack of regulation which involves Factor H. Similarly in AMD altered CFHR1 expression can affect complement regulation in the retinal tissue involving interactions with C3b and Factor H.
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Product protocols

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Target data

Involved in complement regulation. The dimerized forms have avidity for tissue-bound complement fragments and efficiently compete with the physiological complement inhibitor CFH. Can associate with lipoproteins and may play a role in lipid metabolism.
See full target information CFHR1
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websiteProtocolBooklet
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Product promise

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