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AB314849

Human CFHR1 ELISA Kit

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Human CFHR1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CFHR1 with a sensitivity of 5.211 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CFHL, CFHL1, CFHL1P, CFHR1P, FHR1, HFL1, HFL2, CFHR1, Complement factor H-related protein 1, FHR-1, H factor-like protein 1, H36, FHL-1, H-factor-like 1

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Sandwich ELISA - Human CFHR1 ELISA Kit (AB314849)
  • sELISA

Supplier Data

Sandwich ELISA - Human CFHR1 ELISA Kit (AB314849)

Interpolated concentrations of human CFHR1 in serum, plasma (EDTA), plasma (Citrate), and plasma (Heparin)

Interpolated concentration of native CFHR1 was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 20,000 serum, 1 : 20,000 plasma (EDTA), 1 : 40,000 plasma (Citrate), and 1 : 20,000 plasma (Heparin). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human CFHR1 ELISA Kit (AB314849)
  • sELISA

Supplier Data

Sandwich ELISA - Human CFHR1 ELISA Kit (AB314849)

Example of human CFHR1 standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Serum, EDTA Plasma, Citrate plasma, Heparin Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 5.211 pg/mL

Range

18.75 - 1200 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB305137

Anti-CFHR1 antibody [EPR25757-16] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-CFHR1 antibody [EPR25757-16] - BSA and Azide free (Detector) (AB305137)

  • 0
  • 0
Proteins & Peptides

AB152006

Recombinant Human CFHR1 protein

  • 0
  • 0
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Reactivity data

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Product details

Human CFHR1 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CFHR1 protein in Serum, EDTA Plasma, Cit Plasma, Hep Plasma. Quantitate Human CFHR1 with 5.211 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.7" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.36" } ]
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Recovery

[ { "sample": "Serum", "range": "109 - 112 %", "average": "= 111" }, { "sample": "EDTA Plasma", "range": "106 - 108 %", "average": "= 107" }, { "sample": "Citrate plasma", "range": "109 - 114 %", "average": "= 111" }, { "sample": "Heparin Plasma", "range": "92 - 109 %", "average": "= 103" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CFHR1 also known as Complement Factor H-Related protein 1 plays a role in the regulation of the complement system. This protein has a molecular mass of approximately 37 kDa. CFHR1 is mainly expressed in the liver and is present in plasma. The protein has structural similarities to Factor H with homologous domains known as short consensus repeats which contribute to its function in modulating complement activity.
Biological function summary

CFHR1 regulates the alternative pathway of the complement system. It can bind to complement component C3b inhibiting its activity and preventing over-activation of the complement cascade. CFHR1 does not function alone; it interacts with other proteins in the complement system although it forms no stable complex. Through these interactions CFHR1 maintains homeostasis in immune responses.

Pathways

CFHR1 participates significantly in the alternative complement pathway. It works alongside other complement factors such as Factor H to ensure balanced complement activation and regulation. CFHR1 indirectly influences the inflammatory response by regulating this pathway linking innate immunity processes with complement-mediated functions.

CFHR1 is linked to atypical hemolytic uremic syndrome (aHUS) and age-related macular degeneration (AMD). In aHUS CFHR1 gene deletions can lead to unrestrained complement activation due to lack of regulation which involves Factor H. Similarly in AMD altered CFHR1 expression can affect complement regulation in the retinal tissue involving interactions with C3b and Factor H.
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Product protocols

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Target data

Involved in complement regulation. The dimerized forms have avidity for tissue-bound complement fragments and efficiently compete with the physiological complement inhibitor CFH. Can associate with lipoproteins and may play a role in lipid metabolism.
See full target information CFHR1
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websiteProtocolBooklet
en
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Product promise

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For full details, please see our Terms & Conditions

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