JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB314850

Human CFHR2 ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Human CFHR2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CFHR2 with a sensitivity of 5.217 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CFHL2, FHR2, HFL3, CFHR2, Complement factor H-related protein 2, FHR-2, DDESK59, H factor-like 3, H factor-like protein 2

2 Images
Sandwich ELISA - Human CFHR2 ELISA Kit (AB314850)
  • sELISA

Supplier Data

Sandwich ELISA - Human CFHR2 ELISA Kit (AB314850)

Example of human CFHR2 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human CFHR2 ELISA Kit (AB314850)
  • sELISA

Supplier Data

Sandwich ELISA - Human CFHR2 ELISA Kit (AB314850)

Interpolated concentration of native CFHR2 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 1 : 8,000, plasma (EDTA) 1 : 4,000, plasma (Citrate) 1 : 4,000, and plasma (Heparin) 1 : 4,000. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Key facts

Detection method

Colorimetric

Sample types

Serum, EDTA Plasma, Citrate plasma, Heparin Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 5.217 pg/mL

Range

18.75 - 1200 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human CFHR2 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CFHR2 protein in Serum, EDTA Plasma, Cit Plasma, Hep Plasma. Quantitate Human CFHR2 with 5.217 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

style
left-column

Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.1" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.45" } ]
style
left-column

Recovery

[ { "sample": "Serum", "range": "95 - 107 %", "average": "= 102" }, { "sample": "EDTA Plasma", "range": "99 - 108 %", "average": "= 102" }, { "sample": "Citrate plasma", "range": "94 - 109 %", "average": "= 104" }, { "sample": "Heparin Plasma", "range": "96 - 104 %", "average": "= 100" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab314850-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB314850-CMP08", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB314850-CMP05", "productcode":"" }, { "size":"2 x 50 mL", "name":"Sample Diluent NS", "number":"AB314850-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate", "number":"AB314850-CMP07", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB314850-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CFHR2 Capture Antibody", "number":"AB314850-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human CFHR2 Lyophilized Recombinant Protein", "number":"AB314850-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB314850-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CFHR2 Detector Antibody", "number":"AB314850-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pre-Coated 96-Well Microplate", "number":"AB314850-CMP09", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CFHL2 also known as Complement Factor H-Related 2 is a small protein with a mass of approximately 29 kDa. It belongs to the family of complement factor H-related proteins. This protein is expressed predominantly in the liver and also in several other tissues including the blood. Its mechanical function involves binding to certain molecules in the extracellular matrix and surfaces which affects the regulation of the complement system.
Biological function summary

CFHL2 plays a role in the immune system by participating in the regulation of the complement activation. It does not operate alone but is part of a group of proteins that modulate the complement system which helps clear pathogens from an organism. CFHL2 can act by competing with complement factor H potentially altering the activity of the complement system and influencing immune responses.

Pathways

CFHL2 interacts with the alternative pathway of the complement system. This pathway is one of three that facilitate the complement system an important part of innate immunity. Its interaction with complement factor H influences the level of activity within this pathway impacting how the body responds to pathogens. CFHL2 in this way indirectly connects to other proteins regulating the immune response.

CFHL2 has been associated with age-related macular degeneration and atypical hemolytic uremic syndrome. In these conditions an imbalance in the complement system is observed where CFHL2's role in modulating complement factor H comes into play. Such disruption in balance contributes to the pathology of these diseases and implicates related proteins like complement factor H in disease progression.
style
left-column

Product protocols

style
left-column

Target data

Involved in complement regulation. The dimerized forms have avidity for tissue-bound complement fragments and efficiently compete with the physiological complement inhibitor CFH. Can associate with lipoproteins and may play a role in lipid metabolism.
See full target information CFHR2
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Primary Antibodies

AB303639

Anti-CFHR2 antibody [EPR25758-10] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-CFHR2 antibody [EPR25758-10] - BSA and Azide free (Capture) (AB303639)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com