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AB315313

Human CFHR3 ELISA Kit

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Human CFHR3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CFHR3 with a sensitivity of 8.523 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CFHL3, FHR3, CFHR3, Complement factor H-related protein 3, FHR-3, DOWN16, H factor-like protein 3

2 Images
Sandwich ELISA - Human CFHR3 ELISA Kit (AB315313)
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Supplier Data

Sandwich ELISA - Human CFHR3 ELISA Kit (AB315313)

Example of human CFHR3 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human CFHR3 ELISA Kit (AB315313)
  • sELISA

Supplier Data

Sandwich ELISA - Human CFHR3 ELISA Kit (AB315313)

Interpolated concentration of native CFHR3 was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 1,000 serum, plasma (EDTA), plasma (citrate), and plasma (heparin). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Key facts

Detection method

Colorimetric

Sample types

Serum, EDTA Plasma, Citrate plasma, Heparin Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 8.523 pg/mL

Range

31.25 - 2000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

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Cell Lines & Lysates

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Cell Lines & Lysates

AB263149

Human CFHR3 (Complement factor H-related protein 3) knockout A549 cell lysate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human CFHR3 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CFHR3 protein in Serum, EDTA Plasma, Cit Plasma, and Hep Plasma. Quantitate Human CFHR3 with 8.523 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab315065) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2.4" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 7.3" } ]
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Recovery

[ { "sample": "Serum", "range": "100 - 103 %", "average": "= 101" }, { "sample": "EDTA Plasma", "range": "91 - 96 %", "average": "= 94" }, { "sample": "Citrate plasma", "range": "95 - 101 %", "average": "= 99" }, { "sample": "Heparin Plasma", "range": "90 - 106 %", "average": "= 96" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Complement factor H-related protein 3 (CFHR3) also known as FHR-3 plays a role in modulating the complement system. This protein has a mass of approximately 42 kDa. CFHR3 is expressed in tissues like the liver and can be found circulating in the plasma. It belongs to the factor H protein family which participates in maintaining immune homeostasis by regulating complement activation.
Biological function summary

CFHR3 acts as an antagonist to complement factor H by competing for binding sites on target surfaces and complement components. It does not function as a standalone protein but interacts closely within a family that includes CFH and other complement factor H-related proteins. CFHR3 can potentially form complexes with similar proteins to help finely tune the activity of the complement system to prevent excessive immune response.

Pathways

CFHR3 is a part of the complement cascade system specifically the alternative pathway. It contributes to the negative regulation of this pathway which is important for preventing overactivation that could harm host tissues. The protein influences the binding of complement proteins like C3b an essential component in the complement cascade and interacts with other factor H-related proteins such as CFHR1 to modulate pathway activities.

CFHR3's regulatory role connects it to conditions like atypical hemolytic uremic syndrome (aHUS) and age-related macular degeneration (AMD). In aHUS mutations or deletions in CFHR3 sometimes in conjunction with CFHR1 can lead to uncontrolled complement activation contributing to disease progression. Similarly variations in CFHR3's sequence can affect AMD risk often in association with other complement regulatory proteins including CFH indicating its significant position in disease susceptibility through its regulatory function within the immune system.
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Product protocols

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Target data

Might be involved in complement regulation.
See full target information CFHR3
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websiteProtocolBooklet
en
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Product promise

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