Human CFHR4 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CFHR4 with a sensitivity of 7.209 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, EDTA Plasma, Citrate plasma, Heparin Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 21.875 - 1400 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 7.209 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Involved in complement regulation. Can associate with lipoproteins and may play a role in lipid metabolism.
Alternative names
CFHL4, FHR4, CFHR4, Complement factor H-related protein 4, FHR-4
What's included?
Recommended products
Human CFHR4 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CFHR4 with a sensitivity of 7.209 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, EDTA Plasma, Citrate plasma, Heparin Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 21.875 - 1400 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 7.209 pg/mL
- General recovery
- 94%
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 8 | C.V. 7.7 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 3 | C.V. 6.82 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 94 | Range 84 - 107 % |
Sample type EDTA Plasma | Average % = 107 | Range 102 - 111 % |
Sample type Citrate plasma | Average % = 99 | Range 98 - 100 % |
Sample type Heparin Plasma | Average % = 103 | Range 100 - 105 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human CFHR4 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CFHR4 protein in Serum, EDTA Plasma, Cit Plasma, Hep Plasma. Quantitate Human CFHR4 with 7.209 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits: Single-wash protocol reduces assay time to 90 minutes or less -High sensitivity, specificity and reproducibility from superior antibodies -Fully validated in biological samples -96-wells plate breakable into 12 x 8 wells strips A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CFHR4 also known as Complement Factor H-Related 4 is a protein involved in the regulation of the complement system which plays an essential role in innate immunity. CFHR4 has a mass of approximately 86 kDa. The protein is part of the complement factor H protein family and expresses primarily in the liver and plasma. The protein features several short consensus repeat (SCR) domains that facilitate its interaction within the complement pathway therefore influencing immune system activity.
- Biological function summary
CFHR4 modulates the activity of the complement system by binding to C3b a central component in complement activation. It is not a direct inhibitor but competes with Complement Factor H (CFH) for binding sites influencing factor H-mediated regulation. This positioning helps maintain a balance in complement activation and inhibition possibly preventing overactivation which could damage host tissues. CFHR4 does not form large complexes but operates closely with other complement regulators like CFH.
- Pathways
CFHR4 participates in the alternative complement pathway. The alternative pathway acts as a continuous surveillance system that does not require antibody-antigen interactions for activation. CFHR4 interacts with complement components such as C3b and CFH within this pathway contributing to the control and fine-tuning of complement activity under normal physiological conditions. CFH and CFHR4 both play a role ensuring that the complement system responds accurately to threats without damaging host cells.
- Associated diseases and disorders
CFHR4 has implications in diseases like age-related macular degeneration (AMD) and atypical hemolytic uremic syndrome (aHUS). Variants or dysregulation of CFHR4 may disrupt complement control leading to inflammation and tissue damage associated with these conditions. The relationship between CFHR4 CFH and other complement components like C3b reveals the importance of balanced complement control in preventing these diseases. Understanding CFHR4's role assists researchers and clinicians in developing therapeutic strategies for conditions related to complement dysregulation.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Sandwich ELISA - Human CFHR4 ELISA Kit (ab314845)
Interpolated concentration of native CFHR4 was measured in duplicate at different sample concentrations. Undiluted samples are 1:2,000 serum, plasma (citrate), plasma (EDTA), and plasma (heparin). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human CFHR4 ELISA Kit (ab314845)
Example of human CFHR4 standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com