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AB314845

Human CFHR4 ELISA Kit

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Human CFHR4 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CFHR4 with a sensitivity of 7.209 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CFHL4, FHR4, CFHR4, Complement factor H-related protein 4, FHR-4

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Sandwich ELISA - Human CFHR4 ELISA Kit (AB314845)
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Supplier Data

Sandwich ELISA - Human CFHR4 ELISA Kit (AB314845)

Example of human CFHR4 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human CFHR4 ELISA Kit (AB314845)
  • sELISA

Supplier Data

Sandwich ELISA - Human CFHR4 ELISA Kit (AB314845)

Interpolated concentration of native CFHR4 was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 2,000 serum, plasma (citrate), plasma (EDTA), and plasma (heparin).  The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Key facts

Detection method

Colorimetric

Sample types

Serum, EDTA Plasma, Citrate plasma, Heparin Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 7.209 pg/mL

Range

21.875 - 1400 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human CFHR4 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CFHR4 protein in Serum, EDTA Plasma, Cit Plasma, Hep Plasma. Quantitate Human CFHR4 with 7.209 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits: Single-wash protocol reduces assay time to 90 minutes or less         -High sensitivity, specificity and reproducibility from superior antibodies         -Fully validated in biological samples         -96-wells plate breakable into 12 x 8 wells strips A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.7" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.82" } ]
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Recovery

[ { "sample": "Serum", "range": "84 - 107 %", "average": "= 94" }, { "sample": "EDTA Plasma", "range": "102 - 111 %", "average": "= 107" }, { "sample": "Citrate plasma", "range": "98 - 100 %", "average": "= 99" }, { "sample": "Heparin Plasma", "range": "100 - 105 %", "average": "= 103" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CFHR4 also known as Complement Factor H-Related 4 is a protein involved in the regulation of the complement system which plays an essential role in innate immunity. CFHR4 has a mass of approximately 86 kDa. The protein is part of the complement factor H protein family and expresses primarily in the liver and plasma. The protein features several short consensus repeat (SCR) domains that facilitate its interaction within the complement pathway therefore influencing immune system activity.
Biological function summary

CFHR4 modulates the activity of the complement system by binding to C3b a central component in complement activation. It is not a direct inhibitor but competes with Complement Factor H (CFH) for binding sites influencing factor H-mediated regulation. This positioning helps maintain a balance in complement activation and inhibition possibly preventing overactivation which could damage host tissues. CFHR4 does not form large complexes but operates closely with other complement regulators like CFH.

Pathways

CFHR4 participates in the alternative complement pathway. The alternative pathway acts as a continuous surveillance system that does not require antibody-antigen interactions for activation. CFHR4 interacts with complement components such as C3b and CFH within this pathway contributing to the control and fine-tuning of complement activity under normal physiological conditions. CFH and CFHR4 both play a role ensuring that the complement system responds accurately to threats without damaging host cells.

CFHR4 has implications in diseases like age-related macular degeneration (AMD) and atypical hemolytic uremic syndrome (aHUS). Variants or dysregulation of CFHR4 may disrupt complement control leading to inflammation and tissue damage associated with these conditions. The relationship between CFHR4 CFH and other complement components like C3b reveals the importance of balanced complement control in preventing these diseases. Understanding CFHR4's role assists researchers and clinicians in developing therapeutic strategies for conditions related to complement dysregulation.
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Product protocols

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Target data

Involved in complement regulation. Can associate with lipoproteins and may play a role in lipid metabolism.
See full target information CFHR4
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websiteProtocolBooklet
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Complementary Products

Primary Antibodies

AB314306

Anti-CFHR4 antibody [EPR25760-116] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CFHR4 antibody [EPR25760-116] - BSA and Azide free (AB314306)

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