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AB314835

Human CFHR5 ELISA Kit

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Human CFHR5 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human CFHR5 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

CFHL5, FHR5, CFHR5, Complement factor H-related protein 5, FHR-5

1 Images
Sandwich ELISA - Human CFHR5 ELISA Kit (AB314835)
  • sELISA

Supplier Data

Sandwich ELISA - Human CFHR5 ELISA Kit (AB314835)

Example of standard curve using ab314835. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.41 ng/mL

Range

0.41 - 100 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human CFHR5 ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CFHR5 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human CFHR5 coated on a 96-well plate.
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Recovery

[ { "sample": "Serum", "range": "80 - 118 %", "average": "= 98" }, { "sample": "Plasma", "range": "74 - 114 %", "average": "= 93.69" }, { "sample": "Cell culture media", "range": "75 - 120 %", "average": "= 98.64" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab314835-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB314835-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human CFHR5 Protein Standard", "number":"AB314835-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human CFHR5 Antibody-coated ELISA Plate", "number":"AB314835-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB314835-CMP07", "productcode":"" }, { "size":"1 x 200 µL", "name":"500X HRP-Streptavidin", "number":"AB314835-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human CFHR5 Detection Antibody", "number":"AB314835-CMP09", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB314835-CMP06", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB314835-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CFHR5 also known as complement factor H-related protein 5 belongs to the complement system and affects immune response. CFHR5 has an approximate mass of 65 kDa. You can find it in plasma and tissues such as the liver and kidney. It plays a role in regulating the alternative complement pathway by interacting with various complement factors inhibiting excessive immune reactions.
Biological function summary

CFHR5 regulates the complement system to prevent damage to host tissues. It does not commonly form complexes but interacts directly with other proteins in the complement system such as complement factor H. The balance maintained by CFHR5 is necessary to control inflammation and assist in removing pathogens without harming body's own cells.

Pathways

The alternative complement pathway involves CFHR5 as a regulatory component. It ensures proper complement activation and prevents unregulated immune responses. CFHR5 engages with proteins such as complement factor H and C3b during pathway modulation. Changes in CFHR5 can impact the balance of immune activation and clearance of cellular debris within this pathway.

Mutations or malfunction of CFHR5 associate with certain kidney disorders such as CFHR5 nephropathy and atypical hemolytic uremic syndrome (aHUS). These conditions often link with dysregulation of the complement system. The relationship between CFHR5 and complement factor H is important in maintaining kidney function and avoiding disease development.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in complement regulation. The dimerized forms have avidity for tissue-bound complement fragments and efficiently compete with the physiological complement inhibitor CFH.
See full target information CFHR5
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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