AB272194

Human CG beta ELISA Kit (CGB3)

Recombinant
SimpleStep
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Human CG beta ELISA Kit (CGB3) is a single-wash 90-min Simplestep used to quantify Human CG beta (CGB3) with a sensitivity of 2.62 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CGB, CGB5, CGB8, CGB3, Choriogonadotropin subunit beta 3, Choriogonadotropin subunit beta, Chorionic gonadotropin chain beta, CG-beta

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Sandwich ELISA - Human CG beta ELISA Kit (CGB3) (AB272194)

sELISA

Supplier Data

Sandwich ELISA - Human CG beta ELISA Kit (CGB3) (AB272194)

Interpolated concentrations of native CG beta in human serum and plasma samples.

The concentrations of CG beta were measured in duplicates, interpolated from the CG beta standard curves and corrected for sample dilution. Undiluted samples are as follows : pregnant serum 1 : 1000 and pregnant plasma (EDTA) 1 : 2000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CG beta concentration was determined to be 666.7 ng/mL in pregnant serum and 1,589 ng/mL in pregnant plasma (EDTA).

sELISA

Supplier Data

Sandwich ELISA - Human CG beta ELISA Kit (CGB3) (AB272194)

Example of human CG beta standard curve in Sample Diluent NS.

The CG beta standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Urine, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 2.62 pg/mL

Range

23.44 - 1500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human CG beta ELISA kit (ab272194) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CG beta protein in serum, plasma and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CG beta with 2.62 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less

-High sensitivity, specificity and reproducibility from superior antibodies

-Fully validated in biological samples

-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Human Chorionic Gonadotropin (hCG) is a complex glycoprotein composed of two glycosylated subunits alpha and beta which are non-covalently associated and normally produced by placenta during pregnancy. Measurement of intact hCG and of the alpha subunit of hCG appears to give similar results in blood and urine but not the levels of beta subunit. The beta subunits are distinct in each of the hormones and confer receptor and biological specificity. CG beta (CGB3) has an essential role in maternal adaption and stimulates the ovaries to synthesize the steroids that are essential for the maintenance of pregnancy. hCG and the free subunits appear not to be useful as serological markers for nontrophoblastic tumors; however, the absolute increase of β-hCG level in choriocarcinoma patients clearly differentiates it from normal pregnancy.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.2" } ]

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Recovery

[ { "sample": "Serum", "range": "85 - 96 %", "average": "= 90" }, { "sample": "EDTA Plasma", "range": "96 - 117 %", "average": "= 105" }, { "sample": "Urine", "range": "85 - 93 %", "average": "= 90" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Human chorionic gonadotropin (hCG) is a glycoprotein hormone composed of two subunits alpha and beta. The beta subunit confers its specific biological activity. Its molecular weight ranges around 36.7 kDa. hCG is produced in the placenta during pregnancy and is also expressed in pituitary cells. Known alternatively as the pregnancy hormone hCG plays a critical role in maintaining the luteal function during the early stages of pregnancy.

Biological function summary

HCG regulates several functions related to reproduction. It supports the corpus luteum in ovaries to secrete progesterone during early pregnancy contributing to the uterine lining's maintenance. Moreover hCG can interact with the luteinizing hormone/chorionic gonadotropin receptor (LHCGR) indicating its involvement in broader reproductive functions. Not as well known hCG may exist in various forms such as hCG intact influencing certain biological pathways differently.

Pathways

Scientists study hCG's role in reproductive signaling and gonadotropin pathways. It interacts closely with luteinizing hormone (LH) with substantial overlap in their pathway functions impacting reproductive health and fertility cycles. hCG's activation of specific receptors like LHCGR plays a role in these pathways connecting it to various fertility-related responses in the human body.

HCG levels can be an indicative marker for certain conditions. Elevated hCG levels may signal trophoblastic diseases such as molar pregnancy or gestational trophoblastic neoplasia. Its abnormal expression might also indicate testicular cancer where it acts as a tumor marker. Both conditions relate to hCG through its shared glycoprotein structure with other hormones like thyroid-stimulating hormone (TSH) which links the hormone to broader endocrine disorder research.

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Product protocols

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Target data

Beta subunit of the human chorionic gonadotropin (hCG). hCG is a complex glycoprotein composed of two glycosylated subunits alpha and beta which are non-covalently associated. The alpha subunit is identical to those in the pituitary gonadotropin hormones (LH, FSH and TSH). The beta subunits are distinct in each of the hormones and confer receptor and biological specificity. Has an essential role in pregnancy and maternal adaptation. Stimulates the ovaries to synthesize the steroids that are essential for the maintenance of pregnancy.

See full target information CGB3

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human CG beta ELISA Kit (CGB3)

Sandwich ELISA - Human CG beta ELISA Kit (CGB3) (AB272194)

Sandwich ELISA - Human CG beta ELISA Kit (CGB3) (AB272194)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Product promise