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AB313530

Human CHI3L2 ELISA Kit

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Human CHI3L2 ELISA Kit is a Sandwich ELISA for the measurement of Human CHI3L2 in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

Chitinase-3-like protein 2, Chondrocyte protein 39, YKL-39, CHI3L2

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Sandwich ELISA - Human CHI3L2 ELISA Kit (AB313530)
  • sELISA

Supplier Data

Sandwich ELISA - Human CHI3L2 ELISA Kit (AB313530)

Example of standard curve using ab313530. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 14.5 pg/mL

Range

14.5 - 4000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Proteins & Peptides

AB152034

Recombinant Human YKL-39 protein

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human CHI3L2 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CHI3L2 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human CHI3L2 coated on a 96-well plate.
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Recovery

[ { "sample": "Serum", "range": "112 - 135 %", "average": "= 122.9" }, { "sample": "Plasma", "range": "110 - 138 %", "average": "= 125.7" }, { "sample": "Cell culture media", "range": "129 - 140 %", "average": "= 133.7" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab313530-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB313530-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB313530-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human CHI3L2 Detection Antibody", "number":"AB313530-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human CHI3L2 Protein Standard", "number":"AB313530-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human CHI3L2 Antibody-coated ELISA Plate", "number":"AB313530-CMP08", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB313530-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"100X HRP-Streptavidin", "number":"AB313530-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB313530-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

YKL-39 also known as Chitinase-3-like protein 2 is a member of the chitinase-like family of proteins with no enzymatic activity. This protein has a molecular weight of approximately 40 kDa. YKL-39 is mostly expressed in cartilage and actively secreted by chondrocytes the cells responsible for cartilage formation and maintenance. One can also detect lower levels of expression in other tissues including the synovium and skin indicating a potential role in joint health and skin biology.
Biological function summary

YKL-39 plays an important role in tissue remodeling and repair especially within the cartilage matrix. It does not form part of a large protein complex but interacts with other matrix proteins to help maintain structural integrity and support cellular responses to tissue damage. YKL-39 contributes to cell proliferation and has been found to affect inflammation pathways making it an important molecule in both normal physiological processes and in response to stress or injury.

Pathways

YKL-39 functions engage in critical roles within the extracellular matrix and inflammatory response pathways. Specifically it associates with the ECM as it relates to cartilage maintenance where it potentially interacts with other proteins such as YKL-40 another chitinase-like protein. YKL-39's involvement is significant in signaling cascades that mediate extracellular matrix homeostasis and modulate inflammation important for maintaining tissue structure and function.

YKL-39 relates closely to osteoarthritis and rheumatoid arthritis. The elevated expression of YKL-39 in cartilage from osteoarthritic patients suggests its involvement in cartilage degeneration and joint inflammation. During these disease processes YKL-39 may exhibit an association with matrix metalloproteinases proteins involved in the degradation of extracellular matrix components. Understanding YKL-39's function enables researchers to explore potential therapeutic targets for cartilage-related conditions.
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Product protocols

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Target data

Lectin that binds chitooligosaccharides and other glycans with high affinity, but not heparin. Has no chitinase activity.
See full target information CHI3L2
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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