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Human Anti-Chikungunya Virus IgG ELISA Kit is a Sandwich (qualitative) ELISA kit for the measurement of Human Anti-Chikungunya Virus IgG in Human in Citrate plasma, Heparin Plasma, Serum samples.

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Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Heparin Plasma, Serum

Assay type

Sandwich (qualitative)

Reactive species

Human

Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Associated Products

Select an associated product type

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What's included?

1 x 96 Tests
Components
20X Washing Solution
1 x 50 mL
Anti-Human (IgG) Coated Microplate (12 x 8 wells)
1 x 1 Unit
Chikungunya Virus IgG Cut-off Control
1 x 2 mL
Chikungunya Virus IgG Negative Control
1 x 1.5 mL
Chikungunya Virus IgG Positive Control
1 x 1.5 mL
Chikungunya Virus antigen Solution 1 (Lyophilized)
1 x 6 Vial
Chikungunya Virus biotinylated antibody Solution 2
1 x 6 mL
Cover Foil
1 x 1 Unit
IgG Sample Diluent
1 x 100 mL
Stop Solution
1 x 15 mL
Streptavidin Conjugate
1 x 6 mL
Strip holder
1 x 1 Unit
TMB Substrate Solution
1 x 15 mL

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Human Anti-Chikungunya Virus IgG ELISA Kit is a Sandwich (qualitative) ELISA kit for the measurement of Human Anti-Chikungunya Virus IgG in Human in Citrate plasma, Heparin Plasma, Serum samples.

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Heparin Plasma, Serum

Assay type

Sandwich (qualitative)

Reactive species

Human

Assay Platform

Microplate

Precision

Intra assay

Sample

Low + Serum

n

23

mean

-

SD

-

C.V.

9.4

Sample

High + Serum

n

24

mean

-

SD

-

C.V.

2.7

Inter assay

Sample

High + Serum

n

3

mean

-

SD

-

C.V.

3.7

Sample

Low + Serum

n

3

mean

-

SD

-

C.V.

10.9

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Abcam's anti-Chikungunya Virus IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Chikungunya Virus in Human serum and plasma.

A 96-well plate has been precoated with anti-Human antibodies to bind to corresponding antibodies of the sample. Controls or test samples are added to the wells and incubated. Following washing, the Chikungunya Virus antigen is added to the wells and incubated. The wells are then washed as before and the biotinylated Chikungunya Virus antibodies are added to each well followed by incubation. After a last wash step, the streptavidin peroxidase (SP) conjugate which binds to the biotinylated Chikungunya Virus-specific antibodies is added to the wells. TMB is then catalyzed by the SP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Chikungunya Virus IgG sample captured in plate.

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