JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB177848

Human Anti-Chikungunya Virus IgM ELISA Kit

Be the first to review this product! Submit a review

|

(12 Publications)

Human Anti-Chikungunya Virus IgM ELISA Kit is an sandwich ELISA for the qualitative detection of IgM class antibodies against Chikungunya Virus in human plasma and serum samples.

- Colorimetric readout - 450 nm - Works on any standard plate reader
- Easy results interpretation
- Cut-off, positive and negative controls included

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Heparin Plasma, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Qualitative

Assay Platform

Microplate

style
left-column

Complementary Products

Assay Kits

AB177835

Human Anti-Chikungunya Virus IgG ELISA Kit

  • 0
  • 0
Assay Kits

AB195215

Human IgG ELISA Kit

SimpleStep

Sandwich ELISA - Human IgG ELISA Kit (AB195215)

  • 4
  • 6
Assay Kits

AB214568

Human IgM ELISA Kit

SimpleStep

ELISA - Human IgM ELISA Kit (AB214568)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Abcam's anti-Chikungunya Virus IgM Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgM class antibodies against Chikungunya Virus in Human serum and plasma.

A 96-well plate has been precoated with anti-Human antibodies to bind to corresponding antibodies of the sample. Controls or test samples are added to the wells and incubated. Following washing, the Chikungunya Virus antigen is added to the wells and incubated. The wells are then washed as before and the biotinylated Chikungunya Virus antibodies are added to each well followed by incubation. After a last wash step, the streptavidin peroxidase (SP) conjugate which binds to the biotinylated Chikungunya Virus-specific antibodies is added to the wells. TMB is then catalyzed by the SP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Chikungunya Virus IgM sample captured in plate.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "High + Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.9" }, { "reproducibilityType": "Inter", "sample": "Low + Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.3" }, { "reproducibilityType": "Intra", "sample": "Low + Serum", "replicates": 23, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.8" }, { "reproducibilityType": "Intra", "sample": "High + Serum", "replicates": 24, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.4" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab177848-1x96Tests", "assetComponentDetails": [ { "size":"1 x 6 mL", "name":"Streptavidin Conjugate", "number":"AB177848-CMP11", "productcode":"" }, { "size":"1 x 2 mL", "name":"Chikungunya Virus IgM Cut-off Control", "number":"AB177848-CMP06", "productcode":"" }, { "size":"1 x 1.5 mL", "name":"Chikungunya Virus IgM Positive Control", "number":"AB177848-CMP05", "productcode":"" }, { "size":"1 x 1.5 mL", "name":"Chikungunya IgM Negative Control", "number":"AB177848-CMP07", "productcode":"" }, { "size":"1 x 100 mL", "name":"Sample Diluent", "number":"AB177848-CMP04", "productcode":"" }, { "size":"1 x 6 mL", "name":"Chikungunya Virus biotinylated antibody Solution 2", "number":"AB177848-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"20X Washing Solution", "number":"AB177848-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"Stop Solution", "number":"AB177848-CMP12", "productcode":"" }, { "size":"1 x 15 mL", "name":"TMB Substrate Solution", "number":"AB177848-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Strip holder", "number":"AB177848-CMP13", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Cover Foil", "number":"AB177848-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human (IgM) Coated Microplate (12 x 8 wells)", "number":"AB177848-CMP08", "productcode":"" }, { "size":"6 x 1 Vial", "name":"Chikungunya Virus antigen Solution 1 (Lyophilized)", "number":"AB177848-CMP09", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column
style
left-column

Product protocols

style
left-column

Target data

style
left-column

Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Tropical medicine and infectious disease 8: PubMed37104340

2023

Clinical Diagnosis of Chikungunya Infection: An Essential Aid in a Primary Care Setting Where Serological Confirmation Is Not Available.

Applications

Unspecified application

Species

Unspecified reactive species

Juan C Rueda,Ingris Peláez-Ballestas,Jose-Ignacio Angarita,Ana M Santos,Carlos Pinzon,Eugenia-Lucia Saldarriaga,Jorge M Rueda,Elias Forero,Diego L Saaibi,Paula X Pavía,Marta Juliana Mantilla,Gustavo Rodríguez-Salas,Juan Camilo Santacruz,Igor Rueda,Mario H Cardiel,John Londono

PLoS neglected tropical diseases 15:e0009961 PubMed34851949

2021

Evidence of Chikungunya virus seroprevalence in Myanmar among dengue-suspected patients and healthy volunteers in 2013, 2015, and 2018.

Applications

Unspecified application

Species

Unspecified reactive species

Elizabeth Ajema Chebichi Luvai,Aung Kyaw Kyaw,Nundu Sabiti Sabin,Fuxun Yu,Saw Wut Hmone,Kyaw Zin Thant,Shingo Inoue,Kouichi Morita,Mya Myat Ngwe Tun

Transboundary and emerging diseases 69:e895-e905 PubMed34752688

2021

Can presence of HLA type I and II alleles be associated with clinical spectrum of CHIKV infection?

Applications

Unspecified application

Species

Unspecified reactive species

Juan C Rueda,Ana M Santos,Jose-Ignacio Angarita,Eugenia-Lucia Saldarriaga,Ingris Peláez-Ballestas,Alejandro Silva Espinosa,Ignacio Briceño-Balcázar,Sofia Arias-Correal,Jose Arias-Correal,Catalina Villota-Erazo,Viviana Reyes,Santiago Bernal-Macías,Mario H Cardiel,John Londono

International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases 102:97-102 PubMed33075526

2020

Dengue-chikungunya coinfection outbreak in children from Cali, Colombia in 2018-2019.

Applications

Unspecified application

Species

Unspecified reactive species

Jaime E Castellanos,Nayeli Jaimes,Carolina Coronel-Ruiza,Juan Pablo Rojas,Luis F Mejía,Víctor H Villarreal,Luz E Maya,Luz M Claros,Cielo Orjuela,Eliana Calvo,María Victoria Muñoz,Myriam L Velandia-Romero

JCI insight 5: PubMed32155134

2020

Antibody response patterns in chikungunya febrile phase predict protection versus progression to chronic arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Kaustuv Nayak,Vineet Jain,Manpreet Kaur,Naushad Khan,Kamalvishnu Gottimukkala,Charu Aggarwal,Rohit Sagar,Shipra Gupta,Ramesh Chandra Rai,Kritika Dixit,Mohammad Islamuddin,Wajihul Hasan Khan,Anil Verma,Deepti Maheshwari,Yadya M Chawla,Elluri Seetharami Reddy,Harekrushna Panda,Pragati Sharma,Priya Bhatnagar,Prabhat Singh,Siva Raghavendhar B,Ashok Kumar Patel,Vinod H Ratageri,Anmol Chandele,Pratima Ray,Kaja Murali-Krishna

Emerging microbes & infections 8:1490-1500 PubMed31631794

2019

Demographic and clinical characteristics of chikungunya patients from six Colombian cities, 2014-2015.

Applications

Unspecified application

Species

Unspecified reactive species

Juan C Rueda,Ana M Santos,Jose-Ignacio Angarita,Rodrigo B Giraldo,Eugenia-Lucia Saldarriaga,Jesús Giovanny Ballesteros Muñoz,Elías Forero,Hugo Valencia,Francisco Somoza,Daniel Martin-Arsanios,Elias-Josué Quintero,Viviana Reyes-Martinez,Diana Padilla,Francy M Cuervo,Ingris Peláez-Ballestas,Mario H Cardiel,Paula X Pavía,John Londono

Zoonoses and public health 66:909-917 PubMed31449360

2019

Combining anti-IgM and IgG immunoassays for comprehensive chikungunya virus diagnostic testing.

Applications

Unspecified application

Species

Unspecified reactive species

Emelissa J Mendoza,Alyssia Robinson,Kristina Dimitrova,Nicole Mueller,Kimberly Holloway,Kai Makowski,Heidi Wood

Viruses 11: PubMed31052472

2019

Development of an E2 ELISA Methodology to Assess Chikungunya Seroprevalence in Patients from an Endemic Region of Mexico.

Applications

Unspecified application

Species

Unspecified reactive species

Young Chan Kim,César López-Camacho,Nallely Garcia-Larragoiti,Alan Cano-Mendez,Karina Guadalupe Hernandez-Flores,Carlos Alonso Domínguez-Alemán,Maria Antonieta Mar,Héctor Vivanco-Cid,Martha Eva Viveros-Sandoval,Arturo Reyes-Sandoval

The Journal of infectious diseases 214:S471-S474 PubMed27920176

2016

Laboratory Diagnosis of Chikungunya Virus Infections and Commercial Sources for Diagnostic Assays.

Applications

Unspecified application

Species

Unspecified reactive species

Barbara W Johnson,Brandy J Russell,Christin H Goodman

PLoS pathogens 12:e1005891 PubMed27736984

2016

Dysregulated TGF-β Production Underlies the Age-Related Vulnerability to Chikungunya Virus.

Applications

Unspecified application

Species

Unspecified reactive species

Jennifer L Uhrlaub,Vesna Pulko,Victor R DeFilippis,Rebecca Broeckel,Daniel N Streblow,Gary D Coleman,Byung S Park,John F Lindo,Ivan Vickers,Joshua J Anzinger,Janko Nikolich-Žugich
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com