AB246541

Human CHIT1 ELISA Kit

Recombinant
SimpleStep
What is this?

Be the first to review this product! Submit a review

(1 Publication)

Human CHIT1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CHIT1 with a sensitivity of 4.63 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Chitotriosidase-1, Chitinase-1, CHIT1

3 Images

Sandwich ELISA - Human CHIT1 ELISA Kit (AB246541)

sELISA

Lab

Sandwich ELISA - Human CHIT1 ELISA Kit (AB246541)

Example of human CHIT1 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human CHIT1 ELISA Kit (AB246541)

Interpolated concentrations of native CHIT1 in human serum and plasma samples.

The concentrations of CHIT1 were measured in duplicate, interpolated from the CHIT1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 2.5%, plasma (citrate) 2.5%, plasma (EDTA) 2.5%, and plasma (heparin) 2.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CHIT1 concentration was determined to be 23.14 ng/mL in serum, 13.39 ng/mL in plasma (citrate) and 20.15 ng/mL in plasma (EDTA), and 37.12 ng/mL in plasma (heparin).

sELISA

Supplier Data

Sandwich ELISA - Human CHIT1 ELISA Kit (AB246541)

Example of human CHIT1 standard curve in Sample Diluent NS.

The CHIT1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 4.63 pg/mL

Range

15.625 - 1000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

Product details

Human CHIT1 ELISA Kit (ab246541) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CHIT1 protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CHIT1 with 4.63 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

style

left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2" } ]

style

left-column

Recovery

[ { "sample": "Serum", "range": "95 - 101 %", "average": "= 98" }, { "sample": "EDTA Plasma", "range": "102 - 105 %", "average": "= 103" }, { "sample": "Heparin Plasma", "range": "98 - 104 %", "average": "= 101" }, { "sample": "Citrate plasma", "range": "92 - 97 %", "average": "= 95" } ]

style

left-column

What's included?

{ "values": { "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab246541-1x384Tests", "assetComponentDetails": [ { "size":"1 x 250 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB246541-CMP04" }, { "size":"2 x 1 Vial", "name":"Human CHIT1 Lyophilized Recombinant Protein", "number":"AB246541-CMP03" }, { "size":"1 x 600 µL", "name":"10X Human CHIT1 Capture Antibody", "number":"AB246541-CMP01" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB246541-CMP10" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB246541-CMP08" }, { "size":"1 x 600 µL", "name":"10X Human CHIT1 Detector Antibody", "number":"AB246541-CMP02" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB246541-CMP11" }, { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB246541-CMP06" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB246541-CMP07" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB246541-CMP09" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab246541-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB246541-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB246541-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CHIT1 Capture Antibody", "number":"AB246541-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human CHIT1 Lyophilized Recombinant Protein", "number":"AB246541-CMP03", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB246541-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB246541-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB246541-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB246541-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB246541-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CHIT1 Detector Antibody", "number":"AB246541-CMP02", "productcode":"" } ] } } }

style

left-column

Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CHIT1 or chitotriosidase is a glycosyl hydrolase enzyme with significant roles in chitin breakdown. It has an approximate mass of 50 kDa and recognizes chitin-containing substances. CHIT1 predominantly expresses in macrophages and acts as an important enzyme in innate immunity by degrading pathologically accumulated chitin. Alternative names for CHIT1 include chitinase-1 and it is distinguishable by its active role in neutralizing fungal pathogens utilizing this chitinase mechanism.

Biological function summary

CHIT1 participates in inflammatory processes and helps in tissue remodeling which is important in immune responses. CHIT1 does not typically form part of a complex but operates independently to mediate macrophage response through its chitinase activity. It handles polysaccharide elements in cellular debris impacting macrophage activation states. Macrophages utilize CHIT1 to counteract foreign bodies engulfed during phagocytosis playing essential roles in managing fungal infections.

Pathways

CHIT1 influences both the immune response and metabolic pathways through its enzyme function. One key pathway involving CHIT1 is the mannose receptor-mediated clearance pathway which clears glycoproteins from systemic circulation. CHIT1 activity mirrors that of other chitinases like AMCase although they derive from separate genetic backgrounds. CHIT1 contribution enhances macrophage functionality within phagolysosomal pathways important for broad-spectrum pathogen eradication.

CHIT1 correlates significantly with Gaucher's disease and chronic obstructive pulmonary disease (COPD). In Gaucher's disease elevated CHIT1 levels serve as a biomarker indicating macrophage activation and infiltration. In the case of COPD CHIT1 modulates inflammatory responses that contribute to disease progression and respiratory complications. CHIT1 interacts with proteins associated with immune regulation such as TNF-alpha in these diseases impacting inflammatory pathways and disease severities.

style

left-column

Product protocols

style

left-column

Target data

Degrades chitin, chitotriose and chitobiose. May participate in the defense against nematodes and other pathogens. Isoform 3 has no enzymatic activity.

See full target information Chitotriosidase-1

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 15:1444100 PubMed39381000

2024

Chitinase 1: a novel therapeutic target in metabolic dysfunction-associated steatohepatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Jung Hoon Cha,Na Ri Park,Sung Woo Cho,Heechul Nam,Hyun Yang,Eun Sun Jung,Jeong Won Jang,Jong Young Choi,Seung Kew Yoon,Pil Soo Sung,Si Hyun Bae

View all publications

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human CHIT1 ELISA Kit

Sandwich ELISA - Human CHIT1 ELISA Kit (AB246541)

Sandwich ELISA - Human CHIT1 ELISA Kit (AB246541)

Sandwich ELISA - Human CHIT1 ELISA Kit (AB246541)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Complementary Products

Assay Kits

Assay Kits

Assay Kits

Secondary Antibodies

Product promise