Human CHIT1 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human CHIT1 in Heparin Plasma, Citrate plasma, Serum, EDTA Plasma samples.
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Key facts
- Detection method
Colorimetric
- Sample types
Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
15.625 - 1000 pg/mL
- Assay time
1h 30m
- Sensitivity
= 4.63 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Degrades chitin, chitotriose and chitobiose. May participate in the defense against nematodes and other pathogens. Isoform 3 has no enzymatic activity.
Alternative names
Chitotriosidase-1, Chitinase-1, CHIT1
What's included?
Recommended products
Human CHIT1 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human CHIT1 in Heparin Plasma, Citrate plasma, Serum, EDTA Plasma samples.
Key facts
- Detection method
Colorimetric
- Sample types
Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
15.625 - 1000 pg/mL
- Assay time
1h 30m
- Assay Platform
Pre-coated microplate (12 x 8 well strips)
- Sensitivity
= 4.63 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 2 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 1 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 98 | Range 95 - 101 % |
Sample type EDTA Plasma | Average % = 103 | Range 102 - 105 % |
Sample type Heparin Plasma | Average % = 101 | Range 98 - 104 % |
Sample type Citrate plasma | Average % = 95 | Range 92 - 97 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Human CHIT1 ELISA Kit (ab246541) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CHIT1 protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CHIT1 with 4.63 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CHIT1 or chitotriosidase is a glycosyl hydrolase enzyme with significant roles in chitin breakdown. It has an approximate mass of 50 kDa and recognizes chitin-containing substances. CHIT1 predominantly expresses in macrophages and acts as an important enzyme in innate immunity by degrading pathologically accumulated chitin. Alternative names for CHIT1 include chitinase-1 and it is distinguishable by its active role in neutralizing fungal pathogens utilizing this chitinase mechanism.
- Biological function summary
CHIT1 participates in inflammatory processes and helps in tissue remodeling which is important in immune responses. CHIT1 does not typically form part of a complex but operates independently to mediate macrophage response through its chitinase activity. It handles polysaccharide elements in cellular debris impacting macrophage activation states. Macrophages utilize CHIT1 to counteract foreign bodies engulfed during phagocytosis playing essential roles in managing fungal infections.
- Pathways
CHIT1 influences both the immune response and metabolic pathways through its enzyme function. One key pathway involving CHIT1 is the mannose receptor-mediated clearance pathway which clears glycoproteins from systemic circulation. CHIT1 activity mirrors that of other chitinases like AMCase although they derive from separate genetic backgrounds. CHIT1 contribution enhances macrophage functionality within phagolysosomal pathways important for broad-spectrum pathogen eradication.
- Associated diseases and disorders
CHIT1 correlates significantly with Gaucher's disease and chronic obstructive pulmonary disease (COPD). In Gaucher's disease elevated CHIT1 levels serve as a biomarker indicating macrophage activation and infiltration. In the case of COPD CHIT1 modulates inflammatory responses that contribute to disease progression and respiratory complications. CHIT1 interacts with proteins associated with immune regulation such as TNF-alpha in these diseases impacting inflammatory pathways and disease severities.
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2 product images
Sandwich ELISA - Human CHIT1 ELISA Kit (ab246541)
Example of human CHIT1 standard curve in Sample Diluent NS.
The CHIT1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human CHIT1 ELISA Kit (ab246541)
Interpolated concentrations of native CHIT1 in human serum and plasma samples.
The concentrations of CHIT1 were measured in duplicate, interpolated from the CHIT1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 2.5%, plasma (citrate) 2.5%, plasma (EDTA) 2.5%, and plasma (heparin) 2.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CHIT1 concentration was determined to be 23.14 ng/mL in serum, 13.39 ng/mL in plasma (citrate) and 20.15 ng/mL in plasma (EDTA), and 37.12 ng/mL in plasma (heparin).
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