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AB315074

Human CHOP ELISA Kit

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Human CHOP ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CHOP / DDIT3 with a sensitivity of 9.88 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CHOP, CHOP10, GADD153, DDIT3, DNA damage-inducible transcript 3 protein, DDIT-3, C/EBP zeta, C/EBP-homologous protein, C/EBP-homologous protein 10, CCAAT/enhancer-binding protein homologous protein, Growth arrest and DNA damage-inducible protein GADD153, CHOP-10

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Sandwich ELISA - Human CHOP ELISA Kit (AB315074)
  • sELISA

Supplier Data

Sandwich ELISA - Human CHOP ELISA Kit (AB315074)

Interpolated concentration of native DDIT3 was measured in duplicate at different sample concentrations. Undiluted samples are 1,000 µg/mL A549 cell extract, 1,000 µg/mL PC-3 cell extract, 1,000 µg/mL treated HeLa cell extract (2 µg/mL tunicamycin for 8 hrs), and 1,000 µg/mL untreated HeLa cell extract. The interpolated dilution factor corrected values are scaled by extract load and plotted (mean +/- SD, n =2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Human CHOP ELISA Kit (AB315074)
  • sELISA

Supplier Data

Sandwich ELISA - Human CHOP ELISA Kit (AB315074)

Example of human DDIT3 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human CHOP ELISA Kit (AB315074)
  • sELISA

Supplier Data

Sandwich ELISA - Human CHOP ELISA Kit (AB315074)

Interpolated concentration of native mouse DDIT3 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : 1,000 µg/mL NIH/3T3 cell extract, 125 µg/mL treated C2C12 (2 µg/mL tunicamycin for 8 hrs) cell extract, and 1,000 µg/mL untreated C2C12 cell extract. The interpolated dilution factor corrected values off the human DDIT3 curve are scaled by extract load and plotted (mean +/- SD, n =2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 9.88 pg/mL

Range

39.063 - 2500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

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Proteins & Peptides

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Primary Antibodies

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Primary Antibodies

AB183546

Anti-p53 (phospho T55) antibody [EPR17729]

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Western blot - Anti-p53 (phospho T55) antibody [EPR17729] (AB183546)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human CHOP SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of DDIT3 protein in Cell Lysate. Quantitate Human CHOP / DDIT3 with 9.88 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Previously called Human DDIT3 ELISA Kit. The name of this product has been changed, there have been no other changes to the product

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.1" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.35" } ]
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Recovery

[ { "sample": "Cell Lysate", "range": "92 - 96 %", "average": "= 94" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab315074-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB315074-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB315074-CMP10", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB315074-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB315074-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB315074-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB315074-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB315074-CMP12", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB315074-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human DDIT3 Capture Antibody", "number":"AB315074-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human DDIT3 Lyophilized Recombinant Protein", "number":"AB315074-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human DDIT3 Detector Antibody", "number":"AB315074-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB315074-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DDIT3 also known as CHOP (C/EBP homologous protein) is a transcription factor commonly expressed in cells under stress conditions such as endoplasmic reticulum stress. It has a molecular weight of approximately 29 kDa. As part of the system that manages cell stress responses DDIT3 expression can occur in various tissues but it is particularly notable in the context of cellular stress regulation. Researchers often use DDIT3 immunohistochemistry to study its presence and expression pattern in different tissues.
Biological function summary

DDIT3 plays a significant role in mediating cellular stress responses and promoting apoptosis when adaptive pathways fail. DDIT3 is not typically part of a multi-subunit complex but it acts in concert with other stress-related proteins to modulate gene expression. By inducing apoptosis DDIT3 helps remove severely damaged cells maintaining overall tissue health. However excessive DDIT3 activity under prolonged stress can lead to cell loss and tissue damage.

Pathways

DDIT3 is involved in the unfolded protein response (UPR) and endoplasmic reticulum stress pathways. It interacts with proteins such as ATF4 and PERK which are part of the mechanism that governs these pathways. DDIT3 induction contributes to the UPR pathway balancing the cell's fate between repair and apoptosis. This balance is important for cell survival under stress and avoids detrimental cellular damage.

DDIT3 has a strong connection with diabetes and neurodegenerative diseases. In diabetes DDIT3 overactivation can result in pancreatic beta-cell apoptosis worsening the disease's progression. Furthermore its involvement in neurodegenerative diseases includes its association with the accumulation of misfolded proteins exacerbating cellular stress conditions. DDIT3's interaction with proteins such as BCL2 further influences disease pathways by enhancing apoptotic signals.
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Product protocols

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Target data

Multifunctional transcription factor in endoplasmic reticulum (ER) stress response (PubMed : 15322075, PubMed : 15775988, PubMed : 19672300). Plays an essential role in the response to a wide variety of cell stresses and induces cell cycle arrest and apoptosis in response to ER stress (PubMed : 15322075, PubMed : 15775988). Plays a dual role both as an inhibitor of CCAAT/enhancer-binding protein (C/EBP) function and as an activator of other genes (By similarity). Acts as a dominant-negative regulator of C/EBP-induced transcription : dimerizes with members of the C/EBP family, impairs their association with C/EBP binding sites in the promoter regions, and inhibits the expression of C/EBP regulated genes (By similarity). Positively regulates the transcription of TRIB3, IL6, IL8, IL23, TNFRSF10B/DR5, PPP1R15A/GADD34, BBC3/PUMA, BCL2L11/BIM and ERO1L (PubMed : 15775988, PubMed : 17709599, PubMed : 20876114, PubMed : 22761832). Negatively regulates; expression of BCL2 and MYOD1, ATF4-dependent transcriptional activation of asparagine synthetase (ASNS), CEBPA-dependent transcriptional activation of hepcidin (HAMP) and CEBPB-mediated expression of peroxisome proliferator-activated receptor gamma (PPARG) (PubMed : 18940792, PubMed : 19672300, PubMed : 20829347). Together with ATF4, mediates ER-mediated cell death by promoting expression of genes involved in cellular amino acid metabolic processes, mRNA translation and the unfolded protein response (UPR) in response to ER stress (By similarity). Inhibits the canonical Wnt signaling pathway by binding to TCF7L2/TCF4, impairing its DNA-binding properties and repressing its transcriptional activity (PubMed : 16434966). Plays a regulatory role in the inflammatory response through the induction of caspase-11 (CASP4/CASP11) which induces the activation of caspase-1 (CASP1) and both these caspases increase the activation of pro-IL1B to mature IL1B which is involved in the inflammatory response (By similarity). Acts as a major regulator of postnatal neovascularization through regulation of endothelial nitric oxide synthase (NOS3)-related signaling (By similarity).
See full target information DDIT3
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websiteProtocolBooklet
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Product promise

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