Human Chorionic Gonadotropin beta ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human Chorionic Gonadotropin beta in Human in Citrate plasma, Heparin Plasma, Serum samples.
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Beta subunit of the human chorionic gonadotropin (hCG). hCG is a complex glycoprotein composed of two glycosylated subunits alpha and beta which are non-covalently associated. The alpha subunit is identical to those in the pituitary gonadotropin hormones (LH, FSH and TSH). The beta subunits are distinct in each of the hormones and confer receptor and biological specificity. Has an essential role for pregnancy and maternal adaptation. Stimulates the ovaries to synthesize the steroids that are essential for the maintenance of pregnancy.
Choriogonadotropin subunit beta 7
Choriogonadotropin subunit beta 7, CGB7
Human Chorionic Gonadotropin beta ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human Chorionic Gonadotropin beta in Human in Citrate plasma, Heparin Plasma, Serum samples.
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 48 | mean - | SD - | C.V. < 7.6 |
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 3 | mean - | SD - | C.V. < 8.8 |
Abcam's Chorionic Gonadotropin beta in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Chorionic Gonadotropin beta in Human serum and plasma.
A 96-well plate has been precoated with anti-Chorionic Gonadotropin beta IgG antibodies. Samples, standards and the Chorionic Gonadotropin beta-HRP conjugate are added to the wells, where Chorionic Gonadotropin beta in the sample and standards binds to the precoated antibody and added Chorionic Gonadotropin beta-HRP conjugate binds to this antibody-Chorionic Gonadotropin beta complex. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is directly proportional to the amount of Chorionic Gonadotropin beta in the sample and the intensity is measured at 450 nm.
Chorionic Gonadotropin beta also known as β-hCG is a major subunit of the human chorionic gonadotropin hormone. It is a glycoprotein with a molecular mass of approximately 22 kD. β-hCG predominantly expresses in placental syncytiotrophoblast cells and appears in the blood and urine. This hormone plays an essential role in reproductive processes and researchers often measure it using β-hCG ELISA kits.
The beta subunit promotes pregnancy maintenance by supporting corpus luteum function and embryo development. β-hCG exists as part of the larger hCG hormone complex which includes an alpha subunit that it shares with other glycoproteins like luteinizing hormone (LH) and follicle-stimulating hormone (FSH). Its binding to LH/CG receptors in the ovary makes it pivotal for progesterone secretion in early pregnancy.
Chorionic Gonadotropin beta engages in reproductive hormone pathways significantly impacting the gonadotropin-releasing hormone (GnRH) signaling pathway. It relates to LH directly and its actions are indispensable in stimulating ovarian androgen secretion. These pathways further link it to progesterone production emphasizing its importance in fertility and pregnancy.
Chorionic Gonadotropin beta has notable connections with gestational trophoblastic diseases including molar pregnancy and choriocarcinoma. Elevated levels of β-hCG also indicate potential testicular cancers in males. Researchers recognize its association with abnormal cellular growth and its measurements are useful in monitoring these malignancies. Furthermore its interaction with LH and FSH places it within hormonal imbalances that can result in fertility issues.
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