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AB178633

Human Chorionic Gonadotropin beta ELISA Kit

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(4 Publications)

Human Chorionic Gonadotropin beta ELISA Kit is an indirect ELISA for the qualitative detection of class antibodies against Chorionic Gonadotropin beta ELISA Kit in human plasma and serum samples.

- Colorimetric readout - 450 nm - Works on any standard plate reader
- Easy results interpretation

View Alternative Names

Choriogonadotropin subunit beta 7, CGB7

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Heparin Plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

> 0.09 mU/mL

Range

1 - 400 mU/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Chorionic Gonadotropin beta in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Chorionic Gonadotropin beta in Human serum and plasma.

A 96-well plate has been precoated with anti-Chorionic Gonadotropin beta IgG antibodies. Samples, standards and the Chorionic Gonadotropin beta-HRP conjugate are added to the wells, where Chorionic Gonadotropin beta in the sample and standards binds to the precoated antibody and added Chorionic Gonadotropin beta-HRP conjugate binds to this antibody-Chorionic Gonadotropin beta complex. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is directly proportional to the amount of Chorionic Gonadotropin beta in the sample and the intensity is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 48, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 7.6" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 8.8" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab178633-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 mL", "name":"Chorionic Gonadotropin beta Standard 0 - 0 mU/mL", "number":"AB178633-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Cover Foil", "number":"AB178633-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Strip holder", "number":"AB178633-CMP15", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Chorionic Gonadotropin beta Coated Microplate (12 x 8 wells)", "number":"AB178633-CMP11", "productcode":"" }, { "size":"1 x 1 mL", "name":"Chorionic Gonadotropin beta Standard 4 - 100 mU/mL", "number":"AB178633-CMP07", "productcode":"" }, { "size":"1 x 15 mL", "name":"TMB Substrate Solution", "number":"AB178633-CMP13", "productcode":"" }, { "size":"1 x 1 mL", "name":"Chorionic Gonadotropin beta Standard 5 - 400 mU/mL", "number":"AB178633-CMP08", "productcode":"" }, { "size":"1 x 50 mL", "name":"Incubation Buffer", "number":"AB178633-CMP12", "productcode":"" }, { "size":"1 x 20 mL", "name":"50X Washing Solution", "number":"AB178633-CMP01", "productcode":"" }, { "size":"1 x 1 mL", "name":"10X Anti-Chorionic Gonadotropin beta HRP Conjugate", "number":"AB178633-CMP10", "productcode":"" }, { "size":"1 x 1 mL", "name":"Chorionic Gonadotropin beta Standard 1 - 1 mU/mL", "number":"AB178633-CMP04", "productcode":"" }, { "size":"1 x 1 mL", "name":"Chorionic Gonadotropin beta Standard 3 - 20 mU/mL", "number":"AB178633-CMP06", "productcode":"" }, { "size":"1 x 1 mL", "name":"Chorionic Gonadotrophin beta Control", "number":"AB178633-CMP09", "productcode":"" }, { "size":"1 x 1 mL", "name":"Chorionic Gonadotropin beta Standard 2 - 5 mU/mL", "number":"AB178633-CMP05", "productcode":"" }, { "size":"1 x 15 mL", "name":"Stop Solution", "number":"AB178633-CMP14", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Chorionic Gonadotropin beta also known as β-hCG is a major subunit of the human chorionic gonadotropin hormone. It is a glycoprotein with a molecular mass of approximately 22 kD. β-hCG predominantly expresses in placental syncytiotrophoblast cells and appears in the blood and urine. This hormone plays an essential role in reproductive processes and researchers often measure it using β-hCG ELISA kits.
Biological function summary

The beta subunit promotes pregnancy maintenance by supporting corpus luteum function and embryo development. β-hCG exists as part of the larger hCG hormone complex which includes an alpha subunit that it shares with other glycoproteins like luteinizing hormone (LH) and follicle-stimulating hormone (FSH). Its binding to LH/CG receptors in the ovary makes it pivotal for progesterone secretion in early pregnancy.

Pathways

Chorionic Gonadotropin beta engages in reproductive hormone pathways significantly impacting the gonadotropin-releasing hormone (GnRH) signaling pathway. It relates to LH directly and its actions are indispensable in stimulating ovarian androgen secretion. These pathways further link it to progesterone production emphasizing its importance in fertility and pregnancy.

Chorionic Gonadotropin beta has notable connections with gestational trophoblastic diseases including molar pregnancy and choriocarcinoma. Elevated levels of β-hCG also indicate potential testicular cancers in males. Researchers recognize its association with abnormal cellular growth and its measurements are useful in monitoring these malignancies. Furthermore its interaction with LH and FSH places it within hormonal imbalances that can result in fertility issues.
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Product protocols

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Target data

Beta subunit of the human chorionic gonadotropin (hCG). hCG is a complex glycoprotein composed of two glycosylated subunits alpha and beta which are non-covalently associated. The alpha subunit is identical to those in the pituitary gonadotropin hormones (LH, FSH and TSH). The beta subunits are distinct in each of the hormones and confer receptor and biological specificity. Has an essential role for pregnancy and maternal adaptation. Stimulates the ovaries to synthesize the steroids that are essential for the maintenance of pregnancy.
See full target information Choriogonadotropin subunit beta 7

Additional targets

Choriogonadotropin subunit beta 7
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

RSC advances 15:19561-19580 PubMed40503310

2025

Discovery of potential small molecule inhibitors against β-hCG: an study with validation.

Applications

Unspecified application

Species

Unspecified reactive species

Shreya Sara Ittycheria,Krishnankutty Chandrika Sivakumar,Dipyaman Patra,Bhavana Ramachandran,R L Neetha,Arathy V Warrier,M A Aiswariya,S Kaviya,Pankaj Suman,Nagarjun Narayanaswamy,Priya Srinivas

Heliyon 10:e37064 PubMed39286065

2024

Direct DNA binding by BRCA1 on β-hCG promoter and its clinical implications.

Applications

Unspecified application

Species

Unspecified reactive species

Neethu Krishnan,Neetha R L,Arathy V Warrier,Induprabha Yadev,Jaimie Anandan,Sankar Sundaram,Arathi Rajan,Prianka Kumari,Shreya Sara Ittycheria,Manasa V G,Serbin Mohammed,Preethamol S,Rakesh Sathish Nair,Priya Srinivas

Bioengineered 13:2777-2790 PubMed35038951

2022

ZBTB24 (Zinc Finger and BTB Domain Containing 24) prevents recurrent spontaneous abortion by promoting trophoblast proliferation, differentiation and migration.

Applications

Unspecified application

Species

Unspecified reactive species

Haibo Ruan,Zhenzhen Dai,Jinyu Yan,Xiaoxi Long,Yi Chen,Youlin Yang,Qian Yang,Jun Zhu,Meiyun Zheng,Xiahui Zhang

Nature 601:600-605 PubMed34856602

2021

Human blastoids model blastocyst development and implantation.

Applications

Unspecified application

Species

Unspecified reactive species

Harunobu Kagawa,Alok Javali,Heidar Heidari Khoei,Theresa Maria Sommer,Giovanni Sestini,Maria Novatchkova,Yvonne Scholte Op Reimer,Gaël Castel,Alexandre Bruneau,Nina Maenhoudt,Jenna Lammers,Sophie Loubersac,Thomas Freour,Hugo Vankelecom,Laurent David,Nicolas Rivron
View all publications
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