Human Chromogranin A ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Chromogranin A with a sensitivity of 20 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
View Alternative Names
Chromogranin-A, CgA, Pituitary secretory protein I, SP-I, CHGA
- ELISA
Supplier Data
ELISA - Human Chromogranin A ELISA Kit (AB270885)
- sELISA
Supplier Data
Sandwich ELISA - Human Chromogranin A ELISA Kit (AB270885)
Interpolated concentrations of native Chromogranin A in human serum, plasma (EDTA), plasma (citrate), and plasma (heparin).
The concentrations of Chromogranin A (CHGA) were measured in duplicates, interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 5%, plasma (EDTA) 5%, plasma(citrate) 5%, plasma (heparin) 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean target concentration was determined to be 56.7 ng/mL in serum, 48.5 ng/mL in plasma (EDTA), 53.0 ng/mL in and plasma (citrate), and 40.1 ng/mL in plasma (heparin).
- sELISA
Supplier Data
Sandwich ELISA - Human Chromogranin A ELISA Kit (AB270885)
Serum from eight individual healthy Chromogranin A male donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Chromogranin A (CHGA) concentration was determined to be 58.8 ng/mL with a range of 10.4 – 171.9 ng/mL.
- sELISA
Supplier Data
Sandwich ELISA - Human Chromogranin A ELISA Kit (AB270885)
Interpolated concentrations of human Chromogranin A in cerebrospinal fluid.
Interpolated concentration of native Chromogranin A was measured in duplicate at different sample concentrations. Undiluted samples are 5% cerebrospinal fluid. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
- sELISA
Supplier Data
Sandwich ELISA - Human Chromogranin A ELISA Kit (AB270885)
Example of human Chromogranin A standard curve in Sample Diluent NS.
The Chromogranin A standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Human Chromogranin A ELISA Kit (AB270885)
Human Chromogranin A standard curve comparison.
Standard Curve comparison between the original Human MMP3 SimpleStep ELISA kit (ab196271) and current Human MMP3 SimpleStep ELISA kit (ab270885).
Complementary Products
Reactivity data
Product details
Human Chromogranin A ELISA Kit ab270885 is a rapid single-wash 90-min Sandwich ELISA to measure Human Chromogranin A in cell culture media, CSF, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 20 pg/mL.
How the assay works
Human Chromogranin A SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human Chromogranin A ELISA Kit ab270885 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-Chromogranin A antibody [EPR21058-86] - BSA and Azide free (Capture) ab272312
- Anti-Chromogranin A antibody [EPR21058-225] - BSA and Azide free (Detector) ab272312
Chromogranin A (CHGA, CgA, also known as Parathyroid secretory protein 1) is a member of the granin family, which are acidic proteins found in the secretory granules of endocrine cells. CHGA consists of ~460 amino acids and is processed into numerous peptides that have diverse activities. Some of the peptides affect the secretion of other hormones, regulate metabolism, and regulate neural pathways that control behavior. CHGA has been shown to be elevated in the blood of cancer patients, to inhibit angiogenesis, and affect tumor response to chemotherapy. It is thought to be a biomarker for neuroendocrine tumors, and increased levels have been detected in patients with lung cancer, prostate cancer, and breast cancer. Elevated CHGA serum levels have also been detected in non-cancer disease state patients with heart and renal failure, hypertension, rheumatoid arthritis, and various other inflammatory diseases.
Precision
Recovery
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Chromogranin A assists in the regulation of secretory pathways across multiple neuroendocrine systems. It plays a role in hormone storage conversion to active peptides and regulates the exocytosis of hormones. CgA acts within a complex contributing to secretory granule biogenesis and influencing intravesicular acidity. As a regulatory component the exact physiological roles are diverse impacting cardiovascular metabolic and immunological processes.
Pathways
Chromogranin A is involved in pathways such as the catecholamine synthesis and release pathway and the serotonergic pathway. Within these pathways it interacts with proteins like secretogranin II and parathyroid hormone (PTH). These interactions modulate neurotransmitter storage and secretion across various systems. CgA's bioactive peptides also contribute to modulating physiological processes like vasoconstriction and insulin regulation linking it closely with these signaling cascades.
Product protocols
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Target data
Product promise
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