Human Chromogranin A ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Chromogranin A with a sensitivity of 20 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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Key facts
- Detection method
- Colorimetric
- Sample types
- Cerebral Spinal Fluid, Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 56 - 3600 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 20 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Pancreastatin. Strongly inhibits glucose induced insulin release from the pancreas. Catestatin. Inhibits catecholamine release from chromaffin cells and noradrenergic neurons by acting as a non-competitive nicotinic cholinergic antagonist (PubMed:15326220). Displays antibacterial activity against Gram-positive bacteria S.aureus and M.luteus, and Gram-negative bacteria E.coli and P.aeruginosa (PubMed:15723172, PubMed:24723458). Can induce mast cell migration, degranulation and production of cytokines and chemokines (PubMed:21214543). Acts as a potent scavenger of free radicals in vitro (PubMed:24723458). May play a role in the regulation of cardiac function and blood pressure (PubMed:18541522). Serpinin. Regulates granule biogenesis in endocrine cells by up-regulating the transcription of protease nexin 1 (SERPINE2) via a cAMP-PKA-SP1 pathway. This leads to inhibition of granule protein degradation in the Golgi complex which in turn promotes granule formation.
Alternative names
Chromogranin-A, CgA, Pituitary secretory protein I, SP-I, CHGA
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Human Chromogranin A ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Chromogranin A with a sensitivity of 20 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Cerebral Spinal Fluid, Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 56 - 3600 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 20 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 2.9 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 4 | mean - | SD - | C.V. 9.9 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cerebral Spinal Fluid | Average % = 104 | Range 99 - 113 % |
Sample type Serum | Average % = 103 | Range 101 - 106 % |
Sample type EDTA Plasma | Average % = 100 | Range 98 - 105 % |
Sample type Cell culture media | Average % = 93 | Range 91 - 94 % |
Sample type Heparin Plasma | Average % = 98 | Range 88 - 114 % |
Sample type Citrate plasma | Average % = 99 | Range 97 - 101 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Chromogranin A ELISA kit (ab270885) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Chromogranin A protein in human serum, plasmas and cell culture supernatants. It uses our proprietary SimpleStep ELISA® technology. Quantitate human Chromogranin A with 20 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Chromogranin A (CHGA, CgA, also known as Parathyroid secretory protein 1) is a member of the granin family, which are acidic proteins found in the secretory granules of endocrine cells. CHGA consists of ~460 amino acids and is processed into numerous peptides that have diverse activities. Some of the peptides affect the secretion of other hormones, regulate metabolism, and regulate neural pathways that control behavior. CHGA has been shown to be elevated in the blood of cancer patients, to inhibit angiogenesis, and affect tumor response to chemotherapy. It is thought to be a biomarker for neuroendocrine tumors, and increased levels have been detected in patients with lung cancer, prostate cancer, and breast cancer. Elevated CHGA serum levels have also been detected in non-cancer disease state patients with heart and renal failure, hypertension, rheumatoid arthritis, and various other inflammatory diseases.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Chromogranin A (CgA) sometimes called chromagranin A is a glycoprotein with a molecular weight of approximately 49–52 kDa. It is highly expressed in the secretory granules of neuroendocrine cells present in tissues such as the adrenal medulla pancreas and endocrine cells of the gastrointestinal tract. CgA identified as part of a family including chromogranin B and chromogranin C functions mechanically as a precursor to various bioactive peptides. This translates into its involvement in the storage and release of hormones and peptides within vesicles.
- Biological function summary
Chromogranin A assists in the regulation of secretory pathways across multiple neuroendocrine systems. It plays a role in hormone storage conversion to active peptides and regulates the exocytosis of hormones. CgA acts within a complex contributing to secretory granule biogenesis and influencing intravesicular acidity. As a regulatory component the exact physiological roles are diverse impacting cardiovascular metabolic and immunological processes.
- Pathways
Chromogranin A is involved in pathways such as the catecholamine synthesis and release pathway and the serotonergic pathway. Within these pathways it interacts with proteins like secretogranin II and parathyroid hormone (PTH). These interactions modulate neurotransmitter storage and secretion across various systems. CgA's bioactive peptides also contribute to modulating physiological processes like vasoconstriction and insulin regulation linking it closely with these signaling cascades.
- Associated diseases and disorders
Chromogranin A is a known marker for neuroendocrine tumors where its levels in blood may increase due to tumor activity. It links to diseases like pheochromocytoma and carcinoid tumors where improper regulation of CgA-associated pathways occurs. Additionally its connection to parathyroid disorders through the PTH pathway illustrates its broader implication in endocrine pathophysiology. CgA serves as an invaluable diagnostic and prognostic tool in these disease states and supports the development of targeted therapies.
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5 product images
Sandwich ELISA - Human Chromogranin A ELISA Kit (ab270885)
Human Chromogranin A standard curve comparison.
Standard Curve comparison between the original Human MMP3 SimpleStep ELISA kit (ab196271) and current Human MMP3 SimpleStep ELISA kit (ab270885).
Sandwich ELISA - Human Chromogranin A ELISA Kit (ab270885)
Example of human Chromogranin A standard curve in Sample Diluent NS.
The Chromogranin A standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Chromogranin A ELISA Kit (ab270885)
Interpolated concentrations of native Chromogranin A in human serum, plasma (EDTA), plasma (citrate), and plasma (heparin).
The concentrations of Chromogranin A (CHGA) were measured in duplicates, interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 5%, plasma (EDTA) 5%, plasma(citrate) 5%, plasma (heparin) 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean target concentration was determined to be 56.7 ng/mL in serum, 48.5 ng/mL in plasma (EDTA), 53.0 ng/mL in and plasma (citrate), and 40.1 ng/mL in plasma (heparin).
Sandwich ELISA - Human Chromogranin A ELISA Kit (ab270885)
Serum from eight individual healthy Chromogranin A male donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Chromogranin A (CHGA) concentration was determined to be 58.8 ng/mL with a range of 10.4 – 171.9 ng/mL.
Sandwich ELISA - Human Chromogranin A ELISA Kit (ab270885)
Interpolated concentrations of human Chromogranin A in cerebrospinal fluid.
Interpolated concentration of native Chromogranin A was measured in duplicate at different sample concentrations. Undiluted samples are 5% cerebrospinal fluid. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
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