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AB326385

Human Chromogranin A ELISA Kit, Chemiluminescent

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Human Chromogranin A ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human Chromogranin A with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 4.94 - 10800 pg/ml with a sensitivity of 1.62pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
-Patent pending

View Alternative Names

Chromogranin-A, CgA, Pituitary secretory protein I, SP-I, CHGA

6 Images
Sandwich ELISA - Human Chromogranin A ELISA Kit, Chemiluminescent (AB326385)
  • sELISA

Lab

Sandwich ELISA - Human Chromogranin A ELISA Kit, Chemiluminescent (AB326385)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human Chromogranin A ELISA Kit, Chemiluminescent (AB326385)
  • sELISA

Lab

Sandwich ELISA - Human Chromogranin A ELISA Kit, Chemiluminescent (AB326385)

Standard curve comparison between the Chemiluminescent Human Chromagranin A SimpleStep ELISA and colorimetric Human Chromagranin A SimpleStep ELISA (ab270885) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Chromogranin A ELISA Kit, Chemiluminescent (AB326385)
  • sELISA

Lab

Sandwich ELISA - Human Chromogranin A ELISA Kit, Chemiluminescent (AB326385)

Dynamic range comparison between the Chemiluminescent Human Chromagranin A SimpleStep ELISA, colorimetric Human Chromagranin A SimpleStep ELISA (ab270885), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Sandwich ELISA - Human Chromogranin A ELISA Kit, Chemiluminescent (AB326385)
  • sELISA

Supplier Data

Sandwich ELISA - Human Chromogranin A ELISA Kit, Chemiluminescent (AB326385)

Serum from eight individual healthy Chromogranin A male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean Chromogranin A (CHGA) concentration was determined to be 58.8 ng/mL with a range of 10.4 – 171.9 ng/mL.

Sandwich ELISA - Human Chromogranin A ELISA Kit, Chemiluminescent (AB326385)
  • sELISA

Supplier Data

Sandwich ELISA - Human Chromogranin A ELISA Kit, Chemiluminescent (AB326385)

Interpolated concentrations of native Chromogranin A in human serum plasma (EDTA) plasma (citrate) and plasma (heparin).

The concentrations of Chromogranin A (CHGA) were measured in duplicates interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 5% plasma (EDTA) 5% plasma(citrate) 5% plasma (heparin) 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean target concentration was determined to be 56.7 ng/mL in serum 48.5 ng/mL in plasma (EDTA) 53.0 ng/mL in and plasma (citrate) and 40.1 ng/mL in plasma (heparin).

Sandwich ELISA - Human Chromogranin A ELISA Kit, Chemiluminescent (AB326385)
  • sELISA

Supplier Data

Sandwich ELISA - Human Chromogranin A ELISA Kit, Chemiluminescent (AB326385)

Interpolated concentrations of human Chromogranin A in cerebrospinal fluid.

Interpolated concentration of native Chromogranin A was measured in duplicate at different sample concentrations. Undiluted samples are 5% cerebrospinal fluid. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). Sample dilutions are made in Sample Diluent NS.

Key facts

Detection method

Luminescent

Sample types

Cerebral Spinal Fluid, Serum, EDTA Plasma, Cell culture media, Heparin Plasma, Citrate plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 1.62 pg/mL

Range

4.94 - 10800 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

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Product details

Human Chromogranin A ELISA Kit, Chemiluminescent (ab326385) is a rapid single-wash sandwich 55-min ELISA to measure Human Chromogranin A in Cerebral Spinal Fluid, Serum, EDTA Plasma, Cell culture media, Heparin Plasma, Citrate plasma with a sensitivity of 1.62 pg/mL.

How the assay works

Human Chromogranin A ELISA Kit, Chemiluminescent (ab326385) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Chromogranin A (ab326385) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-CHGA antibody [EPR21058-86] - BSA and Aside free (Capture) ab272311
- Anti-CHGA antibody [EPR21058-225] - BSA and Aside free (Detector) ab272312
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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2.9" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 4, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 9.9" } ]
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Recovery

[ { "sample": "Cerebral Spinal Fluid", "range": "99 - 113 %", "average": "= 104" }, { "sample": "Serum", "range": "101 - 106 %", "average": "= 103" }, { "sample": "EDTA Plasma", "range": "98 - 105 %", "average": "= 100" }, { "sample": "Cell culture media", "range": "91 - 94 %", "average": "= 93" }, { "sample": "Heparin Plasma", "range": "88 - 114 %", "average": "= 98" }, { "sample": "Citrate plasma", "range": "97 - 101 %", "average": "= 99" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Pancreastatin. Strongly inhibits glucose induced insulin release from the pancreas.. Catestatin. Inhibits catecholamine release from chromaffin cells and noradrenergic neurons by acting as a non-competitive nicotinic cholinergic antagonist (PubMed : 15326220). Displays antibacterial activity against Gram-positive bacteria S.aureus and M.luteus, and Gram-negative bacteria E.coli and P.aeruginosa (PubMed : 15723172, PubMed : 24723458). Can induce mast cell migration, degranulation and production of cytokines and chemokines (PubMed : 21214543). Acts as a potent scavenger of free radicals in vitro (PubMed : 24723458). May play a role in the regulation of cardiac function and blood pressure (PubMed : 18541522).. Serpinin. Regulates granule biogenesis in endocrine cells by up-regulating the transcription of protease nexin 1 (SERPINE2) via a cAMP-PKA-SP1 pathway. This leads to inhibition of granule protein degradation in the Golgi complex which in turn promotes granule formation.
See full target information CHGA
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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