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AB304940

Human Chromogranin C/SGII ELISA Kit

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Human Chromogranin C/SGII ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Chromogranin C/SGII with a sensitivity of 13.15 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CHGC, SCG2, Secretogranin-2, Chromogranin-C, Secretogranin II, SgII

2 Images
Sandwich ELISA - Human Chromogranin C/SGII ELISA Kit (AB304940)
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Supplier Data

Sandwich ELISA - Human Chromogranin C/SGII ELISA Kit (AB304940)

Interpolated concentration of native Chromogranin C/SGII was measured in duplicate at different sample concentrations. Undiluted samples are as follows : SH-SY5Y cell extract 5 µg/mL, Human cortex tissue extract 20 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Human Chromogranin C/SGII ELISA Kit (AB304940)
  • sELISA

Supplier Data

Sandwich ELISA - Human Chromogranin C/SGII ELISA Kit (AB304940)

Example of human Chromogranin C/SGII standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Tissue Extracts

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 13.15 pg/mL

Range

85.938 - 5500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Chromogranin C/SGII SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Chromogranin C/SGII protein in cell extracts and tissue lysates. Quantitate Human Chromogranin C/SGII with 13.15 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.6" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.1" } ]
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Recovery

[ { "sample": "Cell Lysate", "range": "92 - 105 %", "average": "= 97" }, { "sample": "Tissue Extracts", "range": "89 - 97 %", "average": "= 93" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Chromogranin C also known as secretogranin II (SGII) is a member of the granin family and weighs approximately 68 kDa. This protein is mainly located within the dense core secretory granules of endocrine and neuronal cells. It plays a mechanical role in the storage and release of peptide hormones and neurotransmitters. Chromogranin C is found in various regions of the brain adrenal medulla and in the pituitary gland reflecting its involvement in the neuroendocrine system.
Biological function summary

This protein functions in the regulation of peptide hormone secretion and is part of a complex with other granin proteins such as Chromogranin A. It modulates the granule's environment by affecting the pH and ionic composition which influences the storage and release of hormones and neurotransmitters. Chromogranin C participates in the production of biologically active peptides after proteolytic processing playing roles in the modulation of endocrine and neuronal activities.

Pathways

Chromogranin C connects to the neuroendocrine signaling pathways and regulated secretory pathway. Within these pathways it interacts with other proteins such as the prohormone convertases and furin which handle peptide precursors' cleavage into active hormones and neurotransmitters. This procession is important for maintaining cellular communication and response mechanisms linking Chromogranin C to cellular signaling and biochemical processes that control physiological homeostasis and stress responses.

Chromogranin C correlates with neurodegenerative diseases like Alzheimer's disease and psychiatric disorders such as depression. These connections arise through its relationship with amyloid precursor protein in Alzheimer's and alterations in serotonergic systems in depression. The expression levels and processing of Chromogranin C may serve as a biomarker for these diseases highlighting its potential role in the pathogenesis and progression of neuro-psychiatric conditions.
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Product protocols

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Target data

Neuroendocrine protein of the granin family that regulates the biogenesis of secretory granules.
See full target information SCG2
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websiteProtocolBooklet
en
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Complementary Products

Primary Antibodies

AB275019

Anti-Chromogranin C/SGII antibody [EPR23876-52]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|Trial Size

Flow Cytometry (Intracellular) - Anti-Chromogranin C/SGII antibody [EPR23876-52] (AB275019)

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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