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AB309450

Human cIAP1 ELISA Kit

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Human cIAP1 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human cIAP1 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

API1, MIHB, RNF48, BIRC2, Baculoviral IAP repeat-containing protein 2, Cellular inhibitor of apoptosis 1, IAP homolog B, Inhibitor of apoptosis protein 2, RING finger protein 48, RING-type E3 ubiquitin transferase BIRC2, TNFR2-TRAF-signaling complex protein 2, C-IAP1, hIAP-2, hIAP2

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Sandwich ELISA - Human cIAP1 ELISA Kit (AB309450)
  • sELISA

Supplier Data

Sandwich ELISA - Human cIAP1 ELISA Kit (AB309450)

Example of standard curve using ab309450. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 1.65 ng/mL

Range

1.65 - 400 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human cIAP1 ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement ofHuman cIAP1 in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human cIAP1 coated on a 96-well plate.
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Recovery

[ { "sample": "Serum", "range": "74 - 147 %", "average": "= 100.4" }, { "sample": "Plasma", "range": "67 - 91 %", "average": "= 77.55" }, { "sample": "Cell culture supernatant", "range": "68 - 80 %", "average": "= 72.59" }, { "sample": "Cell culture media", "range": "68 - 80 %", "average": "= 72.59" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab309450-1x96Tests", "assetComponentDetails": [ { "size":"1 x 30 mL", "name":"Assay Diluent C (Item L)", "number":"AB309450-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human cIAP-1/BIRC2/hIAP-2 Protein Standard", "number":"AB309450-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB309450-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB309450-CMP11", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B (Item E)", "number":"AB309450-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human cIAP-1/BIRC2/hIAP-2 Antibody-coated ELISA Plate", "number":"AB309450-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human cIAP-1/BIRC2/hIAP-2 Detection Antibody", "number":"AB309450-CMP08", "productcode":"" }, { "size":"1 x 200 µL", "name":"500X HRP-Streptavidin", "number":"AB309450-CMP13", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB309450-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The cellular inhibitor of apoptosis protein 1 (cIAP1) also referred to as BIRC2 is a protein with a molecular mass of approximately 70 kDa. cIAP1 belongs to the inhibitor of apoptosis (IAP) family and contains several distinct domains important for its function including three Baculoviral IAP Repeat (BIR) domains a ubiquitin-associated (UBA) domain and a really interesting new gene (RING) domain. This protein is expressed in various tissues but predominantly in cells engaged in apoptosis regulation such as immune cells.
Biological function summary

CIAP1 plays an important role in regulating apoptosis by inhibiting the activity of certain caspases including caspase-3 and caspase-7. It is often part of larger protein complexes that include other members of the IAP family such as cIAP2. By binding directly to tumor necrosis factor receptor-associated factors (TRAFs) cIAP1 helps modulate signaling pathways that determine cell survival or death contributing to cellular homeostasis.

Pathways

CIAP1 is intricately involved in the NF-κB signaling pathway and the TNF receptor signaling pathway. Its interaction with TRAFs links it to these critical pathways helping to control inflammatory and immune responses. Other proteins related to cIAP1 through these pathways include RIP1 and TRAF2 both important for transmitting signals that affect cell fate decisions.

CIAP1 has significant implications in cancer and autoimmune disorders. In many cancers overexpression of cIAP1 contributes to uncontrolled cell proliferation by preventing apoptosis therefore aiding tumor survival and progression. Moreover its dysregulation is linked to disorders such as rheumatoid arthritis where improper cell death regulation leads to tissue damage. cIAP1 interacts with and regulates the activity of the protein XIAP in contributing to these diseases making it a potential target for therapeutic interventions.
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Product protocols

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Target data

Multi-functional protein which regulates not only caspases and apoptosis, but also modulates inflammatory signaling and immunity, mitogenic kinase signaling, and cell proliferation, as well as cell invasion and metastasis. Acts as an E3 ubiquitin-protein ligase regulating NF-kappa-B signaling and regulates both canonical and non-canonical NF-kappa-B signaling by acting in opposite directions : acts as a positive regulator of the canonical pathway and suppresses constitutive activation of non-canonical NF-kappa-B signaling. The target proteins for its E3 ubiquitin-protein ligase activity include : RIPK1, RIPK2, RIPK3, RIPK4, CASP3, CASP7, CASP8, TRAF2, DIABLO/SMAC, MAP3K14/NIK, MAP3K5/ASK1, IKBKG/NEMO, IKBKE and MXD1/MAD1. Can also function as an E3 ubiquitin-protein ligase of the NEDD8 conjugation pathway, targeting effector caspases for neddylation and inactivation. Acts as an important regulator of innate immune signaling via regulation of Toll-like receptors (TLRs), Nodlike receptors (NLRs) and RIG-I like receptors (RLRs), collectively referred to as pattern recognition receptors (PRRs). Protects cells from spontaneous formation of the ripoptosome, a large multi-protein complex that has the capability to kill cancer cells in a caspase-dependent and caspase-independent manner. Suppresses ripoptosome formation by ubiquitinating RIPK1 and CASP8. Can stimulate the transcriptional activity of E2F1. Plays a role in the modulation of the cell cycle.
See full target information BIRC2
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websiteProtocolBooklet
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Cell Lines & Lysates

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