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AB314836

Human cIAP2 ELISA Kit

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Human cIAP2 ELISA Kit is a Sandwich ELISA for the measurement of Human cIAP2 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

API2, MIHC, RNF49, BIRC3, Baculoviral IAP repeat-containing protein 3, Apoptosis inhibitor 2, Cellular inhibitor of apoptosis 2, IAP homolog C, Inhibitor of apoptosis protein 1, RING finger protein 49, RING-type E3 ubiquitin transferase BIRC3, TNFR2-TRAF-signaling complex protein 1, C-IAP2, hIAP-1, hIAP1

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Sandwich ELISA - Human cIAP2 ELISA Kit (AB314836)
  • sELISA

Supplier Data

Sandwich ELISA - Human cIAP2 ELISA Kit (AB314836)

Example of standard curve using ab314836. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 1 ng/mL

Range

1 - 250 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB32059

Anti-cIAP2 antibody [E40]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-cIAP2 antibody [E40] (AB32059)

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  • 2
Proteins & Peptides

AB268366

Recombinant human cIAP2 protein (Active)

Functional Studies - Recombinant human cIAP2 protein (Active) (AB268366)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human cIAP2 ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human cIAP2 in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human cIAP2 coated on a 96-well plate.
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Recovery

[ { "sample": "Serum", "range": "81 - 98 %", "average": "= 89.48" }, { "sample": "Plasma", "range": "76 - 89 %", "average": "= 82.86" }, { "sample": "Cell culture media", "range": "78 - 103 %", "average": "= 91.7" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab314836-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB314836-CMP04", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB314836-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human cIAP2 Antibody-coated ELISA Plate", "number":"AB314836-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B (Item E)", "number":"AB314836-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human cIAP2 Detection Antibody", "number":"AB314836-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human cIAP2 Protein Standard", "number":"AB314836-CMP11", "productcode":"" }, { "size":"1 x 200 µL", "name":"600X HRP-Streptavidin", "number":"AB314836-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB314836-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D (Item K)", "number":"AB314836-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CIAP2 also called E3 ubiquitin-protein ligase and encoded by the BIRC3 gene functions to inhibit apoptosis. This protein belongs to the inhibitors of apoptosis (IAP) family and is around 69 kDa in mass. cIAP2 is widely expressed in various tissues including the epithelial and lymphoid cells. It operates mechanically by ubiquitinating substrates marking them for proteasomal degradation which is key in preventing cell death and maintaining cellular homeostasis.
Biological function summary

CIAP2 serves as a regulator in cell survival by functioning within signaling cascades. It participates in the formation of the TNF receptor complex which influences the NF-κB signaling pathway and inflammation response. By interacting with several proteins cIAP2 modulates downstream signaling effects that can influence immune responses and cellular proliferation helping cells adapt and survive stressful conditions.

Pathways

CIAP2 plays roles in both the NF-κB and the MAPK signaling pathways. Within these pathways cIAP2 interacts closely with proteins such as TRAF2 and RIP1. In the NF-κB pathway it helps in the regulation of inflammatory responses while in the MAPK pathway it contributes to cell growth and differentiation processes. These pathways highlight its function in regulating immune system responses and cell adaptability.

CIAP2 maintains connections with cancer and chronic inflammation. Abnormal expression or mutations of cIAP2 can lead to tumorigenesis due to disrupted apoptotic control linking it closely to cancers like lymphomas and leukemias. Additionally cIAP2 influences inflammatory diseases like Crohn's disease through its interactions with proteins such as NOD2 which further impacts the disease pathology. Proper regulation of cIAP2 is essential in avoiding unwanted cell survival and excessive inflammation highlighting its significance in various pathological conditions.
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Product protocols

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Target data

Multi-functional protein which regulates not only caspases and apoptosis, but also modulates inflammatory signaling and immunity, mitogenic kinase signaling and cell proliferation, as well as cell invasion and metastasis. Acts as an E3 ubiquitin-protein ligase regulating NF-kappa-B signaling and regulates both canonical and non-canonical NF-kappa-B signaling by acting in opposite directions : acts as a positive regulator of the canonical pathway and suppresses constitutive activation of non-canonical NF-kappa-B signaling. The target proteins for its E3 ubiquitin-protein ligase activity include : RIPK1, RIPK2, RIPK3, RIPK4, CASP3, CASP7, CASP8, IKBKE, TRAF1, and BCL10. Acts as an important regulator of innate immune signaling via regulation of Toll-like receptors (TLRs), Nodlike receptors (NLRs) and RIG-I like receptors (RLRs), collectively referred to as pattern recognition receptors (PRRs). Protects cells from spontaneous formation of the ripoptosome, a large multi-protein complex that has the capability to kill cancer cells in a caspase-dependent and caspase-independent manner. Suppresses ripoptosome formation by ubiquitinating RIPK1 and CASP8.
See full target information BIRC3
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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