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AB313533

Human CILP ELISA Kit

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Human CILP ELISA Kit is a Sandwich ELISA for the measurement of Human CILP in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

UNQ602/PRO1188, CILP, Cartilage intermediate layer protein 1, CILP-1, Cartilage intermediate-layer protein

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Sandwich ELISA - Human CILP ELISA Kit (AB313533)
  • sELISA

Supplier Data

Sandwich ELISA - Human CILP ELISA Kit (AB313533)

Example of standard curve using ab313533. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.05 ng/mL

Range

0.053 - 13 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB251162

Anti-CILP antibody [EPR16303] - BSA and Azide free

RabMAb|Recombinant

Western blot - Anti-CILP antibody [EPR16303] - BSA and Azide free (AB251162)

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  • 0
Proteins & Peptides

AB182802

Recombinant Human CILP protein

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human CILP ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CILP in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human CILP coated on a 96-well plate.

Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human 4-1BB Ligand, Activin RIIB, Aminopeptidase P2, BAMBI, BOC, Brevican, Carbonic Anhydrase XII, Carboxypeptidase A2, CD300c (LMIR2), CD320, CDNF, CDO, CHST1, CHST4, CNTF R alpha, CRIM1, CRTAC1, CXADR, Dopa Decarboxylase (DDC), DPPII (QPP, DPP7), DSPG3, EMR2, FCAR (CD89), FCRL1 (FcRH1), FCRL2 (FcRH2), Gas6, GPR56, GPVI, Hepsin, ILT2 (CD85j), Jagged 2, Kirrel3 (NEPH2), KLF4, LAIR1, LAMP, LAMP1 (CD107a), MDGA1, MIS RII, Neurexin 3.

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Recovery

[ { "sample": "Serum", "range": "93 - 145 %", "average": "= 113" }, { "sample": "Plasma", "range": "70 - 134 %", "average": "= 94.64" }, { "sample": "Cell culture supernatant", "range": "69 - 109 %", "average": "= 87.72" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab313533-1x96Tests", "assetComponentDetails": [ { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin", "number":"AB313533-CMP03", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB313533-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B (Item E)", "number":"AB313533-CMP05", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C (Item L)", "number":"AB313533-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB313533-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human CILP Detection Antibody", "number":"AB313533-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human CILP Protein Standard", "number":"AB313533-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human CILP Antibody-coated ELISA Plate", "number":"AB313533-CMP09", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB313533-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Probably plays a role in cartilage scaffolding. May act by antagonizing TGF-beta1 (TGFB1) and IGF1 functions. Has the ability to suppress IGF1-induced proliferation and sulfated proteoglycan synthesis, and inhibits ligand-induced IGF1R autophosphorylation. May inhibit TGFB1-mediated induction of cartilage matrix genes via its interaction with TGFB1. Overexpression may lead to impair chondrocyte growth and matrix repair and indirectly promote inorganic pyrophosphate (PPi) supersaturation in aging and osteoarthritis cartilage.
See full target information CILP
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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