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AB264617

Human CKM ELISA Kit

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(1 Publication)

Human CKM ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CKM with a sensitivity of 18.9 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CKMM, CKM, Creatine kinase M-type, Creatine kinase M chain, Creatine phosphokinase M-type, M-CK, CPK-M

7 Images
ELISA - Human CKM ELISA Kit (AB264617)
  • ELISA

Supplier Data

ELISA - Human CKM ELISA Kit (AB264617)
Sandwich ELISA - Human CKM ELISA Kit (AB264617)
  • sELISA

Supplier Data

Sandwich ELISA - Human CKM ELISA Kit (AB264617)

Tissue specificity of CKM in human tissue extracts.

Human heart (HHH), human skeletal muscle (HSKM), and human brain (HBH) homogenates were extracted following the procedure as described in Section 11. Levels of CKM in 100 μg/mL extract were interpolated from a standard curve in 1X Cell Extraction Buffer.

Sandwich ELISA - Human CKM ELISA Kit (AB264617)
  • sELISA

Supplier Data

Sandwich ELISA - Human CKM ELISA Kit (AB264617)

Example of human CKM standard curve in Sample Diluent NS.

The CKM standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human CKM ELISA Kit (AB264617)
  • sELISA

Supplier Data

Sandwich ELISA - Human CKM ELISA Kit (AB264617)

Standard Curve comparison between the original human CKM SimpleStep ELISA kit and current human CKM SimpleStep ELISA kit. Human CKM standard curve comparison.

Sandwich ELISA - Human CKM ELISA Kit (AB264617)
  • sELISA

Supplier Data

Sandwich ELISA - Human CKM ELISA Kit (AB264617)

Interpolated concentrations of native CKM in human serum and plasma samples.

The concentrations of CKM were measured in duplicates, interpolated from the CKM standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 6.25%, plasma (citrate) 12.5%, plasma (EDTA), and plasma (heparin) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CKM concentration was determined to be 35,863 pg/mL in serum, 14,078 pg/mL in plasma (citrate), 7,412 pg/mL in plasma (EDTA) and 23.485 pg/mL in plasma (heparin).

Sandwich ELISA - Human CKM ELISA Kit (AB264617)
  • sELISA

Supplier Data

Sandwich ELISA - Human CKM ELISA Kit (AB264617)

Serum from ten individual healthy human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CKM concentration was determined to be 41,464 pg/mL with a range of 11,349 – 88,217 pg/mL.

Sandwich ELISA - Human CKM ELISA Kit (AB264617)
  • sELISA

Supplier Data

Sandwich ELISA - Human CKM ELISA Kit (AB264617)

Interpolated concentrations of native CKM in human skeletal muscle tissue sample based on a 500 ng/mL extract load.

The concentrations of CKM were measured in duplicate and interpolated from the CKM standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CKM concentration was determined to be 2,157 pg/mL in skeletal muscle tissue sample.

Key facts

Detection method

Colorimetric

Sample types

Urine, Tissue Extracts, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 18.9 pg/mL

Range

62.5 - 4000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human CKM ELISA Kit ab264617 is a rapid single-wash 90-min Sandwich ELISA to measure Human CKM in citrate plasma, EDTA plasma, heparin plasma, serum, tissue extracts, urine. This SimpleStep sensitivity is 18.9 pg/mL.

How the assay works

Human CKM SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human CKM ELISA Kit ab264617 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Creatine kinase (CK), also known as creatine phosphokinase (CPK) or phospho-creatine kinase, is an enzyme that catalyzes the reversible transfer of a high-energy phosphate group between phosphocreatine and ATP. There are five known CK isozymes, three located in the cytosol (CKMM, CKMB, CKBB) and two in the mitochondria (MiSCK and MiuCK). CK isoenzymes allow for the generation of a large pool of phosphocreatine, considered to be a temporal and spatial ATP buffering system in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. High levels of serum CKM can be found in inherited myophathies such as Duchenne and Becker muscular dystrophy and other acquired pathologies such as rhabdomyolysis, malignant hyperthermia, inflammatory myophaties, severe trauma and acute renal failure. This kit is developed to specifically recognize only CKM.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.3" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.6" } ]
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Recovery

[ { "sample": "Serum", "range": "100 - 120 %", "average": "= 112" }, { "sample": "EDTA Plasma", "range": "102 - 114 %", "average": "= 108" }, { "sample": "Tissue Extracts", "range": "103 - 106 %", "average": "= 105" }, { "sample": "Heparin Plasma", "range": "103 - 106 %", "average": "= 104" }, { "sample": "Citrate plasma", "range": "82 - 86 %", "average": "= 84" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab264617-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB264617-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB264617-CMP11", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB264617-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB264617-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB264617-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CKM Capture Antibody", "number":"AB264617-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human CKM Lyophilized Recombinant Protein", "number":"AB264617-CMP04", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB264617-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CKM Detector Antibody", "number":"AB264617-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB264617-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB264617-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
See full target information CKM
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 22:5454-5462 PubMed33174008

2020

Silencing of long non‑coding RNA HRIM protects against myocardial ischemia/reperfusion injury via inhibition of NF‑κB signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Li Niu,Yunquan Zhao,Shumei Liu,Weiwei Pan
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websiteProtocolBooklet
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