Human CKM ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human CKM in Urine, Tissue Extracts, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma samples.
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- Molecular medicine reports 22:5454-54622020Silencing of long non‑coding RNA HRIM protects against myocardial ischemia/reperfusion injury via inhibition of NF‑κB signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi Niu et. al.PubMed 33174008
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Tissue Extracts, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 62.5 - 4000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 18.9 pg/mL
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Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
Alternative names
CKMM, CKM, Creatine kinase M-type, Creatine kinase M chain, Creatine phosphokinase M-type, M-CK, CPK-M
What's included?
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Human CKM ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human CKM in Urine, Tissue Extracts, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Tissue Extracts, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 62.5 - 4000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 18.9 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 3.6 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 2.3 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 112 | Range 100 - 120 % |
Sample type EDTA Plasma | Average % = 108 | Range 102 - 114 % |
Sample type Tissue Extracts | Average % = 105 | Range 103 - 106 % |
Sample type Heparin Plasma | Average % = 104 | Range 103 - 106 % |
Sample type Citrate plasma | Average % = 84 | Range 82 - 86 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human CKM ELISA Kit (ab264617) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CKM protein in cit plasma, edta plasma, hep plasma, serum, tissue extracts, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CKM with 18.9 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Creatine kinase (CK), also known as creatine phosphokinase (CPK) or phospho-creatine kinase, is an enzyme that catalyzes the reversible transfer of a high-energy phosphate group between phosphocreatine and ATP. There are five known CK isozymes, three located in the cytosol (CKMM, CKMB, CKBB) and two in the mitochondria (MiSCK and MiuCK). CK isoenzymes allow for the generation of a large pool of phosphocreatine, considered to be a temporal and spatial ATP buffering system in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. High levels of serum CKM can be found in inherited myophathies such as Duchenne and Becker muscular dystrophy and other acquired pathologies such as rhabdomyolysis, malignant hyperthermia, inflammatory myophaties, severe trauma and acute renal failure. This kit is developed to specifically recognize only CKM.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
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Full details and terms and conditions can be found here:
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6 product images
Sandwich ELISA - Human CKM ELISA Kit (ab264617)
Standard Curve comparison between the original human CKM SimpleStep ELISA kit and current human CKM SimpleStep ELISA kit. Human CKM standard curve comparison.
Sandwich ELISA - Human CKM ELISA Kit (ab264617)
Example of human CKM standard curve in Sample Diluent NS.
The CKM standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human CKM ELISA Kit (ab264617)
Interpolated concentrations of native CKM in human serum and plasma samples.
The concentrations of CKM were measured in duplicates, interpolated from the CKM standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 6.25%, plasma (citrate) 12.5%, plasma (EDTA), and plasma (heparin) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CKM concentration was determined to be 35,863 pg/mL in serum, 14,078 pg/mL in plasma (citrate), 7,412 pg/mL in plasma (EDTA) and 23.485 pg/mL in plasma (heparin).
Sandwich ELISA - Human CKM ELISA Kit (ab264617)
Interpolated concentrations of native CKM in human skeletal muscle tissue sample based on a 500 ng/mL extract load.
The concentrations of CKM were measured in duplicate and interpolated from the CKM standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CKM concentration was determined to be 2,157 pg/mL in skeletal muscle tissue sample.
Sandwich ELISA - Human CKM ELISA Kit (ab264617)
Tissue specificity of CKM in human tissue extracts.
Human heart (HHH), human skeletal muscle (HSKM), and human brain (HBH) homogenates were extracted following the procedure as described in Section 11. Levels of CKM in 100 μg/mL extract were interpolated from a standard curve in 1X Cell Extraction Buffer.
Sandwich ELISA - Human CKM ELISA Kit (ab264617)
Serum from ten individual healthy human male donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CKM concentration was determined to be 41,464 pg/mL with a range of 11,349 – 88,217 pg/mL.
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