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AB326894

Human CKM ELISA Kit, Chemiluminescent

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Human CKM ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human CKM with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 14.63 - 32000 pg/ml with a sensitivity of 2.67 pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending

View Alternative Names

CKMM, CKM, Creatine kinase M-type, Creatine kinase M chain, Creatine phosphokinase M-type, M-CK, CPK-M

6 Images
Sandwich ELISA - Human CKM ELISA Kit, Chemiluminescent (AB326894)
  • sELISA

Supplier Data

Sandwich ELISA - Human CKM ELISA Kit, Chemiluminescent (AB326894)

Serum from ten individual healthy human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CKM concentration was determined to be 41,464 pg/mL with a range of 11,349 – 88,217 pg/mL.

Sandwich ELISA - Human CKM ELISA Kit, Chemiluminescent (AB326894)
  • sELISA

Supplier Data

Sandwich ELISA - Human CKM ELISA Kit, Chemiluminescent (AB326894)

Tissue specificity of CKM in human tissue extracts.

Human heart (HHH), human skeletal muscle (HSKM), and human brain (HBH) homogenates were extracted following the procedure as described in Section 11. Levels of CKM in 100 μg/mL extract were interpolated from a standard curve in 1X Cell Extraction Buffer.

Sandwich ELISA - Human CKM ELISA Kit, Chemiluminescent (AB326894)
  • sELISA

Supplier Data

Sandwich ELISA - Human CKM ELISA Kit, Chemiluminescent (AB326894)

Interpolated concentrations of native CKM in human skeletal muscle tissue sample based on a 500 ng/mL extract load.

The concentrations of CKM were measured in duplicate and interpolated from the CKM standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CKM concentration was determined to be 2,157 pg/mL in skeletal muscle tissue sample.

Sandwich ELISA - Human CKM ELISA Kit, Chemiluminescent (AB326894)
  • sELISA

Supplier Data

Sandwich ELISA - Human CKM ELISA Kit, Chemiluminescent (AB326894)

Interpolated concentrations of native CKM in human serum and plasma samples.

The concentrations of CKM were measured in duplicates, interpolated from the CKM standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 6.25%, plasma (citrate) 12.5%, plasma (EDTA), and plasma (heparin) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CKM concentration was determined to be 35,863 pg/mL in serum, 14,078 pg/mL in plasma (citrate), 7,412 pg/mL in plasma (EDTA) and 23.485 pg/mL in plasma (heparin).

Sandwich ELISA - Human CKM ELISA Kit, Chemiluminescent (AB326894)
  • sELISA

Lab

Sandwich ELISA - Human CKM ELISA Kit, Chemiluminescent (AB326894)

Standard curve comparison between the Chemiluminescent Human CKM SimpleStep ELISA and colorimetric Human CKM SimpleStep ELISA (ab264617) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human CKM ELISA Kit, Chemiluminescent (AB326894)
  • sELISA

Lab

Sandwich ELISA - Human CKM ELISA Kit, Chemiluminescent (AB326894)

Dynamic range comparison between the Chemiluminescent Human CKM SimpleStep ELISA, colorimetric Human CKM SimpleStep ELISA (ab264617), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity.

Key facts

Detection method

Luminescent

Sample types

Serum, EDTA Plasma, Tissue Extracts, Heparin Plasma, Citrate plasma

Reacts with

Human, Monkey

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 2.67 pg/mL

Range

14.63 - 32000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

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Product details

Human CKM ELISA Kit, Chemiluminescent (ab326894) is a rapid single-wash sandwich 55-min ELISA to measure Human CKM in Serum, EDTA Plasma, Tissue Extracts, Heparin Plasma, Citrate plasma with a sensitivity of 2.67 pg/mL.

How the assay works

Human CKM ELISA Kit, Chemiluminescent (ab326894) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human CKM (ab326894) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Human CKMB (creatine kinase MB [dimer]) antibody [EPR22048-58] - BSA and Aside free (Capture) ab270317
- Anti-Human CKMB (creatine kinase MB [dimer]) antibody [EPR22048-58] - BSA and Aside free (Detector) ab270317
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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.6" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2.3" } ]
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Recovery

[ { "sample": "Serum", "range": "100 - 120 %", "average": "= 120" }, { "sample": "EDTA Plasma", "range": "102 - 114 %", "average": "= 114" }, { "sample": "Tissue Extracts", "range": "103 - 106 %", "average": "= 106" }, { "sample": "Heparin Plasma", "range": "103 - 106 %", "average": "= 106" }, { "sample": "Citrate plasma", "range": "82 - 86 %", "average": "= 86" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
See full target information CKM
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com